Traf3ip2 (NM_134000) Mouse Tagged ORF Clone
CAT#: MG208800
- TrueORF®
Traf3ip2 (tGFP-tagged) - Mouse Traf3 interacting protein 2 (Traf3ip2)
ORF Plasmid: DDK
"NM_134000" in other vectors (4)
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CNY 6,080.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Act1; AI429613; CIKS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208800 representing NM_134000
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGAAGCATTCCCGTGGAGGTTGATGAATCAGAACCATTCCCGAGTCAATTGCTGAAACCGATCC CAGAATACTCTCCAGAAGAGGAATTGGAACCACCTGCCCCCAATACAAGGAACATGGCACCCAGCAGCTT GTCTGTGCTCCAGTGCCCTCCCCTGAAACTGGCAAACCACCAGCCTGTGTCACAACAGGTCACCTGCCTG CGAGCTAAAGTCCTGGAGGAAGGTGAAGCCAGTTTCTTTAGAAGGCACCCTGAACTTGGCAAAGATATTT CCTCATGTTCCTCTGGAGCCAGCGAGCCTGAGTCTGAGCTCGGAGCCCTCCCTCCAGAGCATCGGTTCAC ACTTACGGAAAAACGCAATAGATGGCTGGGATCTCAGCTTTCAGCAGCTTCTCCTGACACTGGCCATGAA TCAGACAAATCAGACCCAAGTTTACCTAATGCTTTGGCAGACTCCTTCAGTGGTGGCCAAGAGATGATGC CCCGGCCCCGGCCCCGGCCTGGGCCCCACAGGCACAGGGCAGCCCCAGATGTGCCCACGATAGACACTGG CTATGATTCCCAGCCCCAAGATGTCCTGGGCATCAGGCAGTTGGAGAGGCCATTGCCCCTCACCTCCTCG TGTTACCTGCAGGACCTCCCTGGGCCTCTGAGGTCCAGGGAATTGCCACCTCAGTTTGAACTTGAGAGGT ATCCAATGAACGCCCAGCTGCTGCCGCCCCATCCTTCCCCACAGGCCCCATGGAACTGTCAGTACTACTG CCCCGGAGGGCCCTACCACCACCAGGTGCCACACGGCCATGGCTACCCTCCAGCAGCAGCCTACCAGCAA GTACTCCAGCCTGCTCTGCCTGGGCAGGTCCTTCCTGGGGCAAGGGCAAGAGGCCCACGCCCTGTGCAGA AGGTCATCCTGAATGACTCCAGCCCCCAAGACCAAGAAGAGAGACCTGCACAGAGAGACTTCTCTTTCCC GAGGCTCCCGAGGGACCAGCTCTACCGCCCACCATCTAATGGAGTGGAAGCCCCTGAGGAGTCCTTGGAC CTTCCTGCAGAGCTGAGACCACATGGTCCCCAGGCTCCATCCCTAGCTGCCGTGCCTAGACCCCCTAGCA ACCCCTTAGCCCGAGGAACTCTAAGAACCAGCAATTTGCCAGAAGAATTACGGAAAGTCTTTATCACTTA TTCTATGGACACAGCCATGGAGGTGGTGAAATTTGTGAACTTTCTGTTGGTGAACGGCTTCCAAACTGCG ATTGACATATTTGAGGATAGAATCCGGGGTATTGATATCATTAAATGGATGGAGCGCTATCTTCGAGATA AGACAGTGATGATAATCGTAGCAATCAGCCCCAAATACAAACAGGATGTGGAAGGCGCTGAGTCGCAGCT GGACGAGGACGAGCATGGCTTACATACTAAGTACATTCATCGGATGATGCAGATTGAGTTCATAAGTCAG GGAAGCATGAACTTCAGATTCATCCCTGTGCTCTTCCCAAATGCCAAGAAGGAGCATGTGCCGACCTGGC TTCAGAACACTCATGTTTACAGCTGGCCCAAGAATAAGAAAAACATCCTGCTGCGGCTGCTCAGGGAGGA AGAGTATGTGGCTCCTCCCCGAGGCCCTCTGCCCACCCTTCAGGTGGTACCCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208800 representing NM_134000
Red=Cloning site Green=Tags(s) MNRSIPVEVDESEPFPSQLLKPIPEYSPEEELEPPAPNTRNMAPSSLSVLQCPPLKLANHQPVSQQVTCL RAKVLEEGEASFFRRHPELGKDISSCSSGASEPESELGALPPEHRFTLTEKRNRWLGSQLSAASPDTGHE SDKSDPSLPNALADSFSGGQEMMPRPRPRPGPHRHRAAPDVPTIDTGYDSQPQDVLGIRQLERPLPLTSS CYLQDLPGPLRSRELPPQFELERYPMNAQLLPPHPSPQAPWNCQYYCPGGPYHHQVPHGHGYPPAAAYQQ VLQPALPGQVLPGARARGPRPVQKVILNDSSPQDQEERPAQRDFSFPRLPRDQLYRPPSNGVEAPEESLD LPAELRPHGPQAPSLAAVPRPPSNPLARGTLRTSNLPEELRKVFITYSMDTAMEVVKFVNFLLVNGFQTA IDIFEDRIRGIDIIKWMERYLRDKTVMIIVAISPKYKQDVEGAESQLDEDEHGLHTKYIHRMMQIEFISQ GSMNFRFIPVLFPNAKKEHVPTWLQNTHVYSWPKNKKNILLRLLREEEYVAPPRGPLPTLQVVPL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134000 |
ORF Size | 1665 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134000.3, NP_598761.2 |
RefSeq Size | 2867 bp |
RefSeq ORF | 1668 bp |
Locus ID | 103213 |
UniProt ID | Q8N7N6 |
Gene Summary | E3 ubiquitin ligase that catalyzes 'Lys63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (By similarity). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (By similarity). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
TRAF3IP2 mediates interleukin-18-induced cardiac fibroblast migration and differentiation
,null,
Cellular signalling
,PubMed ID 23872479
[Traf3ip2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218993 | Traf3ip2 (untagged) - Mouse TRAF3 interacting protein 2 (Traf3ip2), (10ug) |
CNY 6,208.00 |
|
MR208800 | Traf3ip2 (Myc-DDK-tagged) - Mouse TRAF3 interacting protein 2 (Traf3ip2) |
CNY 6,208.00 |
|
MR208800L3 | Lenti ORF clone of Traf3ip2 (Myc-DDK-tagged) - Mouse TRAF3 interacting protein 2 (Traf3ip2) |
CNY 7,410.00 |
|
MR208800L4 | Lenti ORF clone of Traf3ip2 (mGFP-tagged) - Mouse TRAF3 interacting protein 2 (Traf3ip2) |
CNY 7,410.00 |
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