Ccnk (BC027297) Mouse Tagged ORF Clone

CAT#: MG208787

  • TrueORF®

Ccnk (tGFP-tagged) - Mouse cyclin K (cDNA clone MGC:28173 IMAGE:3986609)

ORF Plasmid: DDK tGFP



  "BC027297" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AW123198; AW413594; CPR4; CycK
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208787 representing BC027297
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGAACAAAGAAAACTCAAGCCCTTCAGTCACGTCAGCAAACCTGGACCACACAAAACCATGTT
GGTACTGGGATAAGAAGGACCTAGCGCACACACCCTCACAACTTGAAGGACTCGATCCAGCCACTGAGGC
CCGGTACCGCCGAGAGGGGGCTCGCTTCATCTTTGATGTGGGCACACGTTTGGGGTTACACTATGACACC
CTGGCAAATGGAATCATTTATTTTCACCGATTTTATATGTTCCATTCCTTCAAACAATTCCCAAGATACG
TGACAGGAGCTTGTTGTCTTTTTCTGGCTGGGAAAGTAGAGGAAACGCCAAAAAAGTGTAAAGATATCAT
CAAAACGGCTCGTAGTTTATTAAATGATGTCCAGTTCGGCCAGTTTGGAGATGACCCAAAGGAAGAAGTC
ATGGTGTTGGAGAGGATCTTACTGCAGACCATAAAGTTTGACTTACAAGTAGAGCATCCGTACCAGTTCC
TGCTCAAATATGCAAAGCAGCTCAAAGGTGATAAAAACAAAATTCAGAAGTTGGTTCAAATGGCATGGAC
ATTTGTAAATGACAGTCTCTGCACCACCCTGTCACTGCAGTGGGAACCAGAGATCATAGCTGTCGCAGTA
ATGTATCTTGCGGGACGTTTGTGCAAATTTGAAATACAAGAGTGGACCTCCAAACCCATGTATAGGAGAT
GGTGGGAGCAGTTCGTTCAAGATGTCCCTGTTGACGTTCTGGAAGACATCTGCCACCAAATCCTGGACCT
TTACTCACAAGGGAAACAACAGATGCCTCATCACACCCCTCATCAGCTGCAGCAGCCCCCATCTCTTCAG
CCTACACCACAAGTGCCCCAGGGGCCACAGTCCCAACCATCTCAAGGCTCAGAAGCAGCCCAGCCTCCAC
AGAAGGACTCGCAGCAGTCAGCCCAGCAGCAACAGCAGCAAGCACAACAGCCCAAGAAACCATCCCCTCA
GCCCAGTCCTCCCCGACAGGCCAAGCGCGCCGTGGTGGTTTCTCCCAAGGAAGAGAACAAAGCTACAGAA
CCACCACCACCACCTAAAATTCCCAAACTTGAGGCTACTCACCCACCATTGCCTCCAGCACACCCACCTC
CAGACCGGAAGCCTCCCCTCGCCCCTGCCTTAGGTGAGGCTGAAGCAACTGGTCCTGTGGAAACAAGTGA
TCTCCCCAAAGTCCAGATTCCCCCTCCAGCCCACCCGGCCCCTGTGCACCAGCCCCCACCATTGCCACAT
CGGCCACCACCCCCGCCACCCTCCAGCTACATGACGGGGATGTCCACCACCAGCTCCTACATGTCAGGAG
AGGGCTATCAGAGCCTGCAGTCCATGATGAAGACGGAGGGCCCCTCCTATGGTGCCCTGCCCCCAGCCTC
CTTTCCCCCACCCACCATTCCTCCCCCTACCCCAGGCTACCCTCCCCCTCCCCCTACCTACAACCCCAAT
TTCCCACCCCCACCCCCACGCCTGCCCCCAACTCATGCAGTCCCTCCTCACCCTCCTCCAGGCTTGGGTT
TGCCACCAGCCAGCTACCCACCTCCAGCTGTCCCCCCTGGGGGACAGCCCCCTGTTCCCCCACCCATCCC
CCCACCTGGTATGCCACCTGTTGGGGGGCTAGGGCGGGCGGCCTGGATGAGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208787 representing BC027297
Red=Cloning site Green=Tags(s)

MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDT
LANGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEV
MVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAV
MYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQ
PTPQVPQGPQSQPSQGSEAAQPPQKDSQQSAQQQQQQAQQPKKPSPQPSPPRQAKRAVVVSPKEENKATE
PPPPPKIPKLEATHPPLPPAHPPPDRKPPLAPALGEAEATGPVETSDLPKVQIPPPAHPAPVHQPPPLPH
RPPPPPPSSYMTGMSTTSSYMSGEGYQSLQSMMKTEGPSYGALPPASFPPPTIPPPTPGYPPPPPTYNPN
FPPPPPRLPPTHAVPPHPPPGLGLPPASYPPPAVPPGGQPPVPPPIPPPGMPPVGGLGRAAWMR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC027297
ORF Size 1662 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC027297.1
RefSeq Size 2479 bp
RefSeq ORF 1664 bp
Locus ID 12454
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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