Cnot6 (BC062950) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CCR4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208760 representing BC062950 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTGAGGAAGCAG CAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCTCATCTTT GTGGTCACTCACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCTTCAGACATT GCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGGCAGAACTCG GAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTTTGAGCTGGG AAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTGAACCTCTGT CTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGTTTCAACAGAACAAC CACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGCCTTGTTTTCTGTCAT GTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGTCCATCATGGGCACTT AATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTGATATTATAAGTCTTC AGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACGTGGCTATAATGGATT CTTTAGTCCAAAGTCTCGAGCTCGGACTATGTCTGAACAAGAACGAAAGCATGTTGATGGCTGTGCAATA TTCTTCAAGACGGAAAAATTCACTTTGGTTCAGAAACACACAGTTGAATTTAATCAGCTAGCAATGGCGA ATTCAGAAGGGTCTGAAGCCATGTTGAACAGAGTCATGACAAAGGATAACATTGGAGTTGCAGTACTGCT AGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACAGAAAAACAACTTATT CTCGTGGCTAATGCTCATATGCATTGGGACCCTGAGTACTCAGACGTGAAGTTGGTACAAACTATGATGT TCCTCTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAGTGTATTGGGAGAATG TGGAACTATTCCTCTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGTGTTGTAGAGTATTTG AGCACTGGTGGGGTAGAAACAAATCATAAAGACTTCAAGGAACTGAGGTACAATGAAAGTCTCACAAACT TCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGGATCACTCATGGTTTCAAGTTAAAGAGTGCCTA TGAGAATGGCCTGATGCCTTACACCAATTATACATTTGATTTCAAGGGTATAATTGACTACATCTTCTAT TCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGATCACCATTGGCTAGTCGAGAATAACA TCAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCACTTTTTGCACAATTGGAGCTCTTACT GCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208760 representing BC062950 Red=Cloning site Green=Tags(s) MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSSSLWSLTHLTALHLSDNSLSCIPSDI AKLHNLVYLDLSHNQIQSLPAELGNMVSLRELHLNYNQLRVLPFELGKLFQLQTLSLKGNPLTQDILNLC LEPDGTRRLLNYLLDNLSVSTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYCPSWAL NWDYRKKAIIQEILSCNADIISLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHVDGCAI FFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKELIEMSSGKPHLGTEKQLI LVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRSLKSSVLGECGTIPLVLCADLNSLPDSGVVEYL STGGVETNHKDFKELRYNESLTNFSCNGKNGMTNGRITHGFKLKSAYENGLMPYTNYTFDFKGIIDYIFY SKPQLNTLAILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC062950
ORF Size	1656 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062950, AAH62950
RefSeq Size	2516 bp
RefSeq ORF	1658 bp
Locus ID	104625
Gene Summary	Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function]

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