Cnot6 (BC062950) Mouse Tagged ORF Clone

CAT#: MG208760

  • TrueORF®

Cnot6 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 6 (cDNA clone MGC:77956 IMAGE:6840162)



  "BC062950" in other vectors (1)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,776.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms CCR4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208760 representing BC062950
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTGAGGAAGCAG
CAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCTCATCTTT
GTGGTCACTCACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCTTCAGACATT
GCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGGCAGAACTCG
GAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTTTGAGCTGGG
AAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTGAACCTCTGT
CTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGTTTCAACAGAACAAC
CACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGCCTTGTTTTCTGTCAT
GTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGTCCATCATGGGCACTT
AATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTGATATTATAAGTCTTC
AGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACGTGGCTATAATGGATT
CTTTAGTCCAAAGTCTCGAGCTCGGACTATGTCTGAACAAGAACGAAAGCATGTTGATGGCTGTGCAATA
TTCTTCAAGACGGAAAAATTCACTTTGGTTCAGAAACACACAGTTGAATTTAATCAGCTAGCAATGGCGA
ATTCAGAAGGGTCTGAAGCCATGTTGAACAGAGTCATGACAAAGGATAACATTGGAGTTGCAGTACTGCT
AGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACAGAAAAACAACTTATT
CTCGTGGCTAATGCTCATATGCATTGGGACCCTGAGTACTCAGACGTGAAGTTGGTACAAACTATGATGT
TCCTCTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAGTGTATTGGGAGAATG
TGGAACTATTCCTCTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGTGTTGTAGAGTATTTG
AGCACTGGTGGGGTAGAAACAAATCATAAAGACTTCAAGGAACTGAGGTACAATGAAAGTCTCACAAACT
TCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGGATCACTCATGGTTTCAAGTTAAAGAGTGCCTA
TGAGAATGGCCTGATGCCTTACACCAATTATACATTTGATTTCAAGGGTATAATTGACTACATCTTCTAT
TCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGATCACCATTGGCTAGTCGAGAATAACA
TCAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCACTTTTTGCACAATTGGAGCTCTTACT
GCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208760 representing BC062950
Red=Cloning site Green=Tags(s)

MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSSSLWSLTHLTALHLSDNSLSCIPSDI
AKLHNLVYLDLSHNQIQSLPAELGNMVSLRELHLNYNQLRVLPFELGKLFQLQTLSLKGNPLTQDILNLC
LEPDGTRRLLNYLLDNLSVSTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYCPSWAL
NWDYRKKAIIQEILSCNADIISLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHVDGCAI
FFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKELIEMSSGKPHLGTEKQLI
LVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRSLKSSVLGECGTIPLVLCADLNSLPDSGVVEYL
STGGVETNHKDFKELRYNESLTNFSCNGKNGMTNGRITHGFKLKSAYENGLMPYTNYTFDFKGIIDYIFY
SKPQLNTLAILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062950
ORF Size 1656 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC062950, AAH62950
RefSeq Size 2516 bp
RefSeq ORF 1658 bp
Locus ID 104625
Gene Summary Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...