Cnot6 (BC062950) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CCR4
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC062950 CGGCCGCCGCCATGATGCTTGTTTGCTTTCAATGAAAACGAGGGGGGCGCGGAAGAGGAGGCGTCGGCGT CGGTGGCGGCGGCGTCGACGGCGCGGAGGCGAAGGCGGCGGCCGGCGCGGCGAGGAGGGCGAGGCCGGGG TCCGAGAGGGCGGCAGAGCGTAGCGGCGGCTCGTGGGGTCCGGGGCGGCCGAGGCGGGCGGCTGAGGAGC GGCGGCCGAGGAGGGGGAACAAAGAGCGGCGGCGGAGGCGCTGCAGCAGCGGGCGGGACTGGTATGGTGG TTCCACAGGGCAGACCCCGCTGCACTCATCGGGAGGAGGCGGCGGCAGCGGCGGAGGAAGGCGGCGCAGC TCGAGAGGCATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTG AGGAAGCAGCAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAG CTCATCTTTGTGGTCACTCACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCT TCAGACATTGCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGG CAGAACTCGGAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTT TGAGCTGGGAAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTG AACCTCTGTCTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGTTTCAA CAGAACAACCACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGCCTTGTT TTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGTCCATCA TGGGCACTTAATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTGATATTA TAAGTCTTCAGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACGTGGCTA TAATGGATTCTTTAGTCCAAAGTCTCGAGCTCGGACTATGTCTGAACAAGAACGAAAGCATGTTGATGGC TGTGCAATATTCTTCAAGACGGAAAAATTCACTTTGGTTCAGAAACACACAGTTGAATTTAATCAGCTAG CAATGGCGAATTCAGAAGGGTCTGAAGCCATGTTGAACAGAGTCATGACAAAGGATAACATTGGAGTTGC AGTACTGCTAGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACAGAAAAA CAACTTATTCTCGTGGCTAATGCTCATATGCATTGGGACCCTGAGTACTCAGACGTGAAGTTGGTACAAA CTATGATGTTCCTCTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAGTGTATT GGGAGAATGTGGAACTATTCCTCTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGTGTTGTA GAGTATTTGAGCACTGGTGGGGTAGAAACAAATCATAAAGACTTCAAGGAACTGAGGTACAATGAAAGTC TCACAAACTTCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGGATCACTCATGGTTTCAAGTTAAA GAGTGCCTATGAGAATGGCCTGATGCCTTACACCAATTATACATTTGATTTCAAGGGTATAATTGACTAC ATCTTCTATTCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGATCACCATTGGCTAGTCG AGAATAACATCAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCACTTTTTGCACAATTGGA GCTCTTACTGCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGGTAGTCACTTTCAGAG GACCTCAGATTTACTTGTAAACTCGTGAAAATCTGAATATAGGAGAGTAAGGTATGGCCACTAGGGATTT TTCTCAATGATCATTTGAATTTTCAGTCTGGTTATTTGATAAAGGGTATAGTATGAAAGCCAGGTGCTAG CAACAGACAAATTCTGAGCCCAGTGTACTTTATATACTGCTAGACAGGGACTGGTGTGTTTACACCTTTT AATTTGTTACAAGTAATTTTCCTGTTTCTTCTCTCCAATGTGTTTTGTCATGCCTTGAAATAGGAAGATG TGTGAGCAGTGTGCTGTTTGCACAGAAATCCACAGCACTGTTTTCTGAGGCCAGTAGTAACATCAGAGAC CGTTCTTCCAGACTTTACTCCCTCTTTTTTCAGACTTGTTTGTAAAATGTAGAATTTGAATTTAGCCTTT ATGATTGTATATGATCCACAGAAGACCTGATTTATGAAGTTTTGTACTAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC062950
Insert Size	1659 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062950, AAH62950
RefSeq Size	2516 bp
RefSeq ORF	1659 bp
Locus ID	104625
Gene Summary	Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function]

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