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Rgs14 (NM_016758) Mouse Tagged ORF Clone
CAT＃: MG208709
- TrueORF®
Rgs14 (tGFP-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14)
ORF Plasmid: DDK
"NM_016758" in other vectors (4)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RPIP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208709 representing NM_016758
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGGAAGCCCAAGCACTTGGGTGTCCCCAACGGGCGCATGGTTCTGGCTGTCTCAGATGGAGAAC TGACCAGCACTGCAGGTTCCCAGGCCCAGGGTGAGGGCAGAGGCAGCTCTCTCAGCATCCACAGCCTCCC TAGTGGCCCCAGCAGCCCCTTCTCCACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCAGTCCTTTGAGCGG CTGCTGCAAGACCCACGGGGTCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAATTCAGCGCAGAGAACG TAACTTTCTGGAAAGCCTGCGAACGTTTCCAGCAGATCCCAGCCAGCGACACCAAGCAGCTAGCTCAGGA GGCCCACAACATCTACCACGAGTTCCTATCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGACAGGCC TGGCTTAGTGAGGAGGTGCTGGCCCAGCCCCGGCCAGATATGTTCCGAGCACAGCAGCTTCAGATCTTCA ATTTGATGAAGTTCGACAGCTATGCGCGCTTCGTCAAATCCCCGCTGTACCAAGAGTGCCTGCTGGCGGA GGCCGAGGGACGCCCCCTGCGGGAACCTGGCTCCTCACACCTCGGGAGCCCGGACACAGCGAGGAAGAAG CCAAAGCTGAAGCCTGGGAAGTCACTGCCGCTGGGCGTGGAAGAGTTGGGGCAGCTGCCACTCGCTGAGG GCCCTTGTGGCCGCCCTCTCCGCAAGTCCTTTCGTAGAGAGATGACAGGTGGAGCCATGAATTCGGCCCT GCGACGAGAGTCTCAAGGGTCCCTGAATTCTTCTGCCAGTCTGGACCTGGGTTTCCTTGCCTTTGTGAGC AGCAAATCTGAGAGTCACCGGAAGAGCCTTGGAAGTGGAGAGAGTGAGAGCGAGAGTCGGCCGGGGAAGT ATTGCTGCGTGTATCTACCTGACGGCACGGCTTCCTTGGCCCTGGCTCGACCTGGCCTCACCATCCGAGA CATGCTGGCAGGCATCTGTGAGAAGAGAGGCCTCTCTCTGCCTGACATTAAGGTCTACCTGGTGGGCAAT GAACAGAAGGCCCTGGTCCTGGATCAGGATTGCACCGTGCTGGCAGACCAGGAAGTGCGACTGGAAAACA GGATCACCTTCCAGCTGGAGTTGGTCGGCCTGGAGCGAGTGGTCCGGATCTCAGCTAAGCCCACCAAGCG TCTGCAAGAGGCGCTGCAGCCCATCCTGGCTAAGCATGGCCTGAGCCTGGACCAGGTGGTACTGCACAGG CCAGGAGAGAAGCAGCCCATGGATTTGGAGAATCCAGTGAGCTCAGTGGCCTCACAGACACTGGTTTTGG ACACTCCTCCGGATGCAAAGATGAGTGAAGCCAGAAGCATATCCCCCTGCCGCAGTCAGGGATGCCTCCC AAGAACCCAGACCAAGGACAGTCACCTTCCCCCATCGTCCTCCAGTTTGCTGGTAGAAGATGCCAGTAGT TCTACTGGGAACCGGCAGACCTGTGACATTGAAGGCCTGGTGGAGCTGCTGAATCGGGTGCAGAGCAGCG GGGCCCACGATCAGAGAGGACTTCTTCGCAAAGAGGACCTGGTCCTTCCAGAATTTCTGCAGCTTCCTTC CCAAAGACCAGGCTCTCGGGAGGCTCCACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208709 representing NM_016758
Red=Cloning site Green=Tags(s) MPGKPKHLGVPNGRMVLAVSDGELTSTAGSQAQGEGRGSSLSIHSLPSGPSSPFSTEEQPVASWAQSFER LLQDPRGLAYFTEFLKKEFSAENVTFWKACERFQQIPASDTKQLAQEAHNIYHEFLSSQALSPVNIDRQA WLSEEVLAQPRPDMFRAQQLQIFNLMKFDSYARFVKSPLYQECLLAEAEGRPLREPGSSHLGSPDTARKK PKLKPGKSLPLGVEELGQLPLAEGPCGRPLRKSFRREMTGGAMNSALRRESQGSLNSSASLDLGFLAFVS SKSESHRKSLGSGESESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGN EQKALVLDQDCTVLADQEVRLENRITFQLELVGLERVVRISAKPTKRLQEALQPILAKHGLSLDQVVLHR PGEKQPMDLENPVSSVASQTLVLDTPPDAKMSEARSISPCRSQGCLPRTQTKDSHLPPSSSSLLVEDASS STGNRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPSQRPGSREAPP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016758 |
| ORF Size | 1641 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016758.3 |
| RefSeq Size | 2437 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 51791 |
| UniProt ID | P97492 |
| Gene Summary | Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o) alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204443 | Rgs14 (untagged) - Mouse regulator of G-protein signaling 14 (Rgs14), (10ug) |
CNY 4,080.00 |
|
| MR208709 | Rgs14 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14) |
CNY 4,080.00 |
|
| MR208709L3 | Lenti ORF clone of Rgs14 (Myc-DDK-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14) |
CNY 6,650.00 |
|
| MR208709L4 | Lenti ORF clone of Rgs14 (mGFP-tagged) - Mouse regulator of G-protein signaling 14 (Rgs14) |
CNY 6,650.00 |
