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Ajuba (NM_010590) Mouse Tagged ORF Clone
CAT＃: MG208706
- TrueORF®
Ajuba (tGFP-tagged) - Mouse ajuba (Jub)
ORF Plasmid: DDK
"NM_010590" in other vectors (3)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Jub |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208706 representing NM_010590
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACGGTTAGGGGAGAAAGCCAGTCGTCTGCTAGAAAAGTTGAGACTCTCGGACTCCGGCAGCGCCA AGTTCGGCCGCAGAAAGGGCGAAGCGAGCCGATCTGGGTCTGATGGGACCCCCGGAGCAGGCAAGGGACG CTTAAGCGGGTTGGGGGGACCTAGGAAGTCAGGACACCGTGGAGCGAATGGTGGGCCTGGAGATGAACCT TTGGAACCGGCCAGGGAGCAAGGGCCCCTGGACGCCGAGCGGAACGCACGCGGCTCCTTTGAAGCGCAGC GCTTCGAAGGGTCCTTTCCCGGGGGGCCGCCGCCCACTCGAGCCCTGCCTCTGCCTCTGTCGTCGCCTCC TGATTTTCGGCTGGAGACCACGGCTCCAGCCCTTAGCCCTCGCTCCAGCTTCGCCAGTAGCTCGGCCAGC GATGCGAGCAAGCCATCTAGCCCCCGGGGCAGCCTGCTGCTGGACGGAGCGGGGGCCAGCGGAGCCGGAG GTAGCCGGCCGTGCAGCAATCGGACCAGCGGCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCGCT GCCCGCGGGGCCGTGCCTATTCGGCCTCCCGCTGACCACTGCTCCTGCGGGCTACCCAGGAGGGGCTCCG TCCGCCTACCCGGAGCTCCACGCTGCCCTGGACCGACTATGTGCTCATCGGTCCGTGGGATTCGGCTGCC AGGAGAGCCGTCACTCGTACCCCCCGGCCCTGGGCAGCCCCGGAGCTCTAACCGGAGCCGTGGTGGGAAC AGCGGGTCCTTTGGAGAGACGTGGGGCGCAACCCGGACGACACTCGGTTACAGGCTACGGGGACTGCGCC GCGGGGGCCCGTTACCAGGACGAGCTAACAGCATTGCTGCGTTTGACCGTGGCTACCGGTGGGCGAGAAG CCGGTGCTCGCGGGGAACCCTCGGGGATTGAGCCGTCGGGTCTGGAGGAGTCTCCTGGTCCCTTCGTTCC AGAGGCCTCCCGATCACGGATACGGGAGCCAGAGGCCAGAGAAGATTACTTTGGCACCTGTATCAAGTGC AACAAAGGTATCTATGGGCAGAGCAATGCCTGCCAGGCCCTGGACAGCCTCTACCACACCCAGTGCTTTG TCTGCTGCTCCTGTGGACGAACTTTGCGGTGCAAGGCTTTCTACAGCGTCAATGGCTCTGTCTACTGTGA GGAAGACTATCTGTTTTCAGGGTTTCAGGAGGCAGCTGAGAAGTGCTGTGTCTGTGGCCACTTGATTCTA GAGAAGATCCTCCAGGCCATGGGGAAGTCCTATCACCCGGGCTGCTTCCGATGCATCGTATGTAACAAGT GCCTGGACGGCGTCCCCTTCACTGTGGACTTCTCCAACCAGGTGTACTGTGTTACCGACTACCACAAAAA TTACGCCCCGAAATGTGCAGCCTGCGGACAACCCATCCTCCCCTCAGAGGGCTGTGAGGACATTGTGAGG GTGATATCCATGGACCGTGATTATCACTTTGAGTGCTACCACTGTGAGGACTGCCGCATGCAGCTGAGTG ACGAGGAAGGTTGCTGCTGTTTCCCTCTTGATGGACATTTGCTCTGCCACGGCTGTCACATGCAGCGCCT CAGTGCCCGGCAGCCCTCTACCAACTATATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208706 representing NM_010590
Red=Cloning site Green=Tags(s) MERLGEKASRLLEKLRLSDSGSAKFGRRKGEASRSGSDGTPGAGKGRLSGLGGPRKSGHRGANGGPGDEP LEPAREQGPLDAERNARGSFEAQRFEGSFPGGPPPTRALPLPLSSPPDFRLETTAPALSPRSSFASSSAS DASKPSSPRGSLLLDGAGASGAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGLPLTTAPAGYPGGAP SAYPELHAALDRLCAHRSVGFGCQESRHSYPPALGSPGALTGAVVGTAGPLERRGAQPGRHSVTGYGDCA AGARYQDELTALLRLTVATGGREAGARGEPSGIEPSGLEESPGPFVPEASRSRIREPEAREDYFGTCIKC NKGIYGQSNACQALDSLYHTQCFVCCSCGRTLRCKAFYSVNGSVYCEEDYLFSGFQEAAEKCCVCGHLIL EKILQAMGKSYHPGCFRCIVCNKCLDGVPFTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVR VISMDRDYHFECYHCEDCRMQLSDEEGCCCFPLDGHLLCHGCHMQRLSARQPSTNYI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010590 |
| ORF Size | 1641 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010590.3 |
| RefSeq Size | 3280 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 16475 |
| UniProt ID | Q91XC0 |
| Gene Summary | Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208706 | Ajuba (Myc-DDK-tagged) - Mouse ajuba (Jub) |
CNY 4,080.00 |
|
| MR208706L3 | Lenti ORF clone of Ajuba (Myc-DDK-tagged) - Mouse ajuba (Jub) |
CNY 6,650.00 |
|
| MR208706L4 | Lenti ORF clone of Ajuba (mGFP-tagged) - Mouse ajuba (Jub) |
CNY 6,650.00 |
