Pde1a (BC090628) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI987702; AW125737
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208682 representing BC090628 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCTACTGATACGGACATCGAAGAATTGGAAAACGCCACTTACAAGTATCTCATTGGAGAACAGA CCGAGAAGATGTGGCAGCGCCTGAAGGGAATACTAAGATGCTTAGTGAAGCAGCTGGAGAAAGGTGATGT CAACGTTGTTGACCTAAAGAAGAATATCGAATATGCAGCATCAGTACTGGAAGCTGTTTACATCGATGAG ACAAGAAGACTGCTGGACACAGAGGATGAACTCAGCGACATCCAGACTGACTCCGTCCCATCGGAAGTCC GGGATTGGTTGGCTTCAACTTTCACACGCAAAATGGGGATGATGAAAAAGAAGCCTGAAGAAAAACCAAA GTTTCGCAGCATTGTCCATGCTGTCCAGGCTGGGATTTTTGTGGAAAGGATGTACAGGAAGAACTATCAC ATGGTTGGTTTGACATATCCTGCAGCTGTCATAGTGACACTCAAGGAAGTTGATAAATGGTCTTTTGATG TATTTGCCTTAAATGAAGCAAGCGGGGAGCATAGCCTGAAGTTTATGATTTATGAACTCTTTACCAGATA TGATCTTATCAACCGCTTCAAGATTCCTGTTTCTTGCCTAATTGCCTTTGCAGAAGCCTTAGAAGTTGGT TACAGCAAGCACAAAAATCCATATCATAATTTGGTCCATGCAGCTGACGTCACTCAAACTGTGCATTATA TAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTTTAGCCATGGTCTTTGCAGCTGC CATTCATGATTATGAGCACACAGGAACAACAAACAACTTCCATATTCAGACGAGGTCGGACGTTGCTATT CTGTACAACGACCGCTCAGTGCTTGAGAATCACCACGTCAGCGCAGCCTACAGACTTATGCAAGAGGAGG AAATGAATATTTTGGTAAATTTGTCTAAAGATGACTGGAGGGATCTTCGGAACTTAGTGATTGAAATGGT CTTAGCGACAGACATGTCAGGGCATTTTCAGCAAATTAAAAACATAAGAAACAGCTTGCAGCAGCCGGAA GGGATTGACAGAGCCAAAACTATGTCCCTGATACTCCATGCAGCTGACATCAGCCACCCAGCCAAAACTT GGAAGTTGCACTACAGATGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAGGGAGACAAAGAAGCTGA ATTAGGGCTTCCATTTTCTCCACTTTGTGATCGGAAGTCAACAATGGTTGCCCAGTCGCAAATAGGTTTC ATTGATTTCATAGTGGAGCCAACGTTTTCTCTCCTGACGGACTCAACAGAGAAAATTGTCATTCCTCTTA TAGAGGAAGCTTCAAAGTCACAGTCTTCAAACTACGGGGCAAGCAGCTCGTCCACCATGATTGGGTTCCA TGTTGCTGACTCTCTGAGGCGGTCAAATACAAAAGGCTCCGTGTGTGATGGGAGCTATGCCCCAGACTAC TCGCTCTCAGCTGTGGATCTGAAGAGTTTCAAAAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGA GGTGGAAAGAGTTGGCTGCCCAAGGTGAACTTGATCTTCATAAGAACTCAGAAGAGTTAGGAAATACTGA AGAAAAGCATGCTGACACACGTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208682 representing BC090628 Red=Cloning site Green=Tags(s) MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVVDLKKNIEYAASVLEAVYIDE TRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMMKKKPEEKPKFRSIVHAVQAGIFVERMYRKNYH MVGLTYPAAVIVTLKEVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLIAFAEALEVG YSKHKNPYHNLVHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAI LYNDRSVLENHHVSAAYRLMQEEEMNILVNLSKDDWRDLRNLVIEMVLATDMSGHFQQIKNIRNSLQQPE GIDRAKTMSLILHAADISHPAKTWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGF IDFIVEPTFSLLTDSTEKIVIPLIEEASKSQSSNYGASSSSTMIGFHVADSLRRSNTKGSVCDGSYAPDY SLSAVDLKSFKNNLVDIIQQNKERWKELAAQGELDLHKNSEELGNTEEKHADTRP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC090628
ORF Size	1635 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC090628, AAH90628
RefSeq Size	4255 bp
RefSeq ORF	1637 bp
Locus ID	18573
Gene Summary	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).[UniProtKB/Swiss-Prot Function]

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