Slc29a4 (BC025599) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ENT4, MGC38048
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208471 representing BC025599 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCTATCGGAAGCCAGCGCCTCAAGGAGCCCTGCGTGGCAGCCACATCTGACCAGAGTGTGGTGA CAAGCTTTAGCTTTGACAACTTCCAGCTGGAGACGACAGCAGAGGGGGCTCAGGATCCAGGCATCAGAGT CAGGGGTGTCCCCACTTTCACAGACTCTGCGGTAGAGGAGCCTGTGCCAGATGACCGCTACCATGCCATC TACTTTGCCATGCTGCTGGCTGGCGTGGGCTTCCTACTGCCATACAACAGCTTTATCACCGATGTCGACT ATCTTCACCACAAGTACCCAGGGACCTCCATCGTATTTGACATGAGCCTCACCTATATCCTGGTGGCATT GGCGGCTGTGCTCCTAAACAACGTTGTGGTGGAGAGGTTGAACCTGCACACCAGGATCACCACAGGCTAC CTCCTCGCCTTGGGTCCCTTGCTCTTTATCAGCATATGCGATGTGTGGCTGCAGCTCTTCTCTCACGACC AGGCTTACGCCATCAACCTGGCCGCCGTGGGCACTGTGGCCTTTGGTTGCACAGTGCAGCAATCTAGCTT CTATGGCTACACGGGATTACTGCCCAAGAGGTACACGCAGGGTGTGATGACTGGAGAGAGCACGGCAGGG GTGATGATCTCTCTGAGTCGAATCCTCACTAAGCTGCTGCTCCCGGACGAGCGGGCCAGCACCATCATCT TCTTCCTGGTGTCTGCCGGCTTGGAGCTCCTGTGCTTCCTGCTGCATCTGCTGGTGCGGCGCAGCCGTTT TGTGCTCTACTATACCACTCGGCCCCGTGACAGCCGCCCCGTCCAGGCAGGCTACCGAGTGCACCACGAT GTCGCATCCGGAGACATCCACTTTGAGCACCAAACTCCAGCCCTATCCAGCAGCAGGTCCCCGAAGGAGA GCCCGGCCCATGAGGTGACGCACAGCAACAGTGGGGTCTACATGCGTTTTGATGTGCCCCGGCCACGAGT CAAACGAAGCTGGCCCACCTTCAGAGCCCTGCTGTTGCACCGATACGTCGTGGCGCGGGTCATCTGGGCC GACATGCTGTCCATTGCGGTAACCTACTTCATTACACTGTGCCTGTTTCCGGGCCTGGAGTCCGAGATCC GCCACTGCGTGCTGGGGGAGTGGCTGCCCATCCTGGTGATGGCCGTGTTCAACCTGTCAGACTTTGTGGG CAAGATCCTGGCAGCCCTGCCCGTGGAGTGGCGGGGCACTCACCTGCTCGCCTGCTCTTGCCTGCGTGTG GTCTTCATCCCCCTCTTCATCCTGTGTGTGTACCCCAGTGGCATGCCTGCCCTCCGCCACCCTGCCTGGC CCTGTGTCTTCTCGCTGCTAATGGGCATCAGCAATGGCTACTTTGGCAGTGTGCCCATGATCCTGGCAGC TGGAAAAGTGAGCCCCAAGCAGCGGGAACTGGCAGGAAACACCATGACTGTGTCCTACATGTCGGGGCTG ACCCTGGGCTCTGCTGTGGCCTACTGCACCTACAGCCTCACCCGAGATGCCCACGGCAGCTGCTTTCAAA CAGCCACCGCCGCCGCCGCCAACGACTCCATCCCTGTCGGTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208471 representing BC025599 Red=Cloning site Green=Tags(s) MGSIGSQRLKEPCVAATSDQSVVTSFSFDNFQLETTAEGAQDPGIRVRGVPTFTDSAVEEPVPDDRYHAI YFAMLLAGVGFLLPYNSFITDVDYLHHKYPGTSIVFDMSLTYILVALAAVLLNNVVVERLNLHTRITTGY LLALGPLLFISICDVWLQLFSHDQAYAINLAAVGTVAFGCTVQQSSFYGYTGLLPKRYTQGVMTGESTAG VMISLSRILTKLLLPDERASTIIFFLVSAGLELLCFLLHLLVRRSRFVLYYTTRPRDSRPVQAGYRVHHD VASGDIHFEHQTPALSSSRSPKESPAHEVTHSNSGVYMRFDVPRPRVKRSWPTFRALLLHRYVVARVIWA DMLSIAVTYFITLCLFPGLESEIRHCVLGEWLPILVMAVFNLSDFVGKILAALPVEWRGTHLLACSCLRV VFIPLFILCVYPSGMPALRHPAWPCVFSLLMGISNGYFGSVPMILAAGKVSPKQRELAGNTMTVSYMSGL TLGSAVAYCTYSLTRDAHGSCFQTATAAAANDSIPVGP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025599
ORF Size	1584 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025599, AAH25599
RefSeq Size	2886 bp
RefSeq ORF	1586 bp
Locus ID	243328
Gene Summary	Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia (By similarity).[UniProtKB/Swiss-Prot Function]

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