Ugt2a1 (BC048926) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208466 representing BC048926 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAGAACATTTTGCTCTGTTCTCTTCAGATAAGCCTTCTAGGAATGAGTCTTGGTGGGAACGTTC TCATCTGGCCCATGGAAGGCAGTCACTGGCTAAATGTTAAGATCATAATAGATGAACTCCTAAGAAAAGA GCATAATGTGACTGTCCTTGTGGCCTCCGGGGCCCTTTTCATCACACCATCATCCATCAGCCCATCTCTG ACATTTGAAATATATCCAGTGCCCTTTGGCAAGGAAAAAATTGAAAGTGTGATCAAAGACTTTGTTTTGA CATGGCTGGAAAACAGGCCATCTCCTTCAACTATCTGGACTTTCTATAAGGAAATGGCCAAAGTCATTGA GGAGTTTCATTTGGTGTCCAGAGGTATCTGTGATGGCGTTCTTAAGAATGAAAAGCTGATGTCCAAGCTT CAGAAAGAAAAGTTCGAAGTACTGTTATCAGACCCTGTATTTCCTTGTGGTGACATCGTGGCTTTAAAAC TGGGAATTCCATTTATCTATTCCTTGCGGTTTTCCCCTGCCTCCACAGTGGAAAAGCATTGTGGAAAGGT CCCATTCCCTCCTTCCTATGTACCTGCAATTCTGTCAGAACTCACAGACCAGATGTCCTTCACTGACCGA GTAAGAAACTTCATTTCTTACCGAATGCAGGACTACATGTTTGAAACCCTTTGGAAGCAGTGGGATTCCT ATTATACTAAAGCTTTAGGAAGACCCACCACACTGTGTGAGACTATGGGGAAAGCTGAGATTTGGCTAAT GCGAACCTATTGGGATTTTGAATTTCCTCGTCCATATTTACCGAATTTTGAGTTTGTGGGAGGACTGCAC TGCAAACCTGCCAAGCCTTTACCTAAGGAAATGGAAGAGTTTGTCCAGACCTCAGGGGAACATGGTATTG TGGTGTTTTCTCTGGGGTCAATGGTCAAAAACCTGACAGATGAAAAGGCCAATCTCATTGCCTCAGCCCT TGCCCAGATTCCTCAGAAGGTTTTGTGGCGATACAAAGGAAAGATACCAGACACATTAGGATCCAATACA AGACTGTTTGATTGGATTCCTCAGAATGATCTTCTTGGACATCCCAAAACCAGAGCTTTTATCACCCATG GTGGAACAAATGGAATCTATGAGGCTATTTACCATGGGATCCCTATGGTGGGAGTGCCCATGTTCGCTGA CCAGCCTGACAACATTGCTCACATGAAGGCCAAAGGAGCAGCAGTGGAGGTGAACATGAACACGATGACA AGCTCAGATCTGCTCAATGCTCTGAGAACAGTCATCAATGAGCCATCTTATAAAGAGAATGCCATGAGAC TATCAAGAATCCACCATGACCAGCCAGTGAAGCCCCTGGACCGAGCAGTCTTCTGGATTGAGTTTGTCAT GCGTCACAAAGGAGCCAAGCACCTTCGTGTGGCAGCCCATGACCTCAGCTGGTTTCAGTACCACTCTCTG GATGTAATTGGGTTTCTGCTGGCCTGTGTGGCATCTGCTATATTATTGGTTGCAAAATGTTGTTTGTTTA TATTTCAAAAAGTTGGTAAGACAGGAAAGAAGAAAAAACGTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208466 representing BC048926 Red=Cloning site Green=Tags(s) MLKNILLCSLQISLLGMSLGGNVLIWPMEGSHWLNVKIIIDELLRKEHNVTVLVASGALFITPSSISPSL TFEIYPVPFGKEKIESVIKDFVLTWLENRPSPSTIWTFYKEMAKVIEEFHLVSRGICDGVLKNEKLMSKL QKEKFEVLLSDPVFPCGDIVALKLGIPFIYSLRFSPASTVEKHCGKVPFPPSYVPAILSELTDQMSFTDR VRNFISYRMQDYMFETLWKQWDSYYTKALGRPTTLCETMGKAEIWLMRTYWDFEFPRPYLPNFEFVGGLH CKPAKPLPKEMEEFVQTSGEHGIVVFSLGSMVKNLTDEKANLIASALAQIPQKVLWRYKGKIPDTLGSNT RLFDWIPQNDLLGHPKTRAFITHGGTNGIYEAIYHGIPMVGVPMFADQPDNIAHMKAKGAAVEVNMNTMT SSDLLNALRTVINEPSYKENAMRLSRIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHDLSWFQYHSL DVIGFLLACVASAILLVAKCCLFIFQKVGKTGKKKKRD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC048926
ORF Size	1586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC048926, AAH48926
RefSeq Size	2585 bp
RefSeq ORF	1586 bp
Locus ID	94215
Gene Summary	UDP-glucuronosyltransferases catalyze phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase water solubility and enhance excretion. They are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Active on odorants and seems to be involved in olfaction; it could help clear lipophilic odorant molecules from the sensory epithelium.[UniProtKB/Swiss-Prot Function]

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