Arhgef3 (BC005517) Mouse Tagged ORF Clone

CAT#: MG208408

  • TrueORF®

Arhgef3 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (cDNA clone MGC:7605 IMAGE:3494309)

ORF Plasmid: DDK tGFP



  "BC005517" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1200004I24Rik; 9830169H03Rik; C76747; Xpln
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208408 representing BC005517
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCCAAGGATTACCCCTTCTACCTCACTGTCAAGAGGGCAAACTGCAGCTTGGAGGCGCCCCTGG
GCAGCGGGGTGGCCAAGGACGAGGAACCTAGTAACAAAAGGGTCAAACCCCTTTCCAGAGTCACATCGCT
AGCAAACCTCATTCCACCTGTGAAGACCACACCATTAAAGCGCTTCAGCCAGACCTTGCAGCGGTCCATC
AGCTTCCGCAGCGAGAGCCGCCCCGACATCCTTGCCCCCCGAGCTTGGTCCAGAAATGCCACCTCCTCAA
GCACAAAGCGGAGAGACAGCAAACTGTGGAGTGAGACCTTCGATGTGTGTGTCAATCAGGTGCTCACGGC
CAAGGAGATCAAGAGACAGGAGGCGATCTTTGAACTTTCTCAAGGTGAAGAAGACTTGATAGAGGACTTG
AAATTAGCAAAAAAGGCCTACCACGACCCCATGCTGAAACTCTCCATAATGACGGAACAGGAACTGAATC
AGATTTTTGGGACCCTGGACTCTCTAATTCCTCTCCATGAAGAGCTCCTCAGTCAGCTTCGAGATGTTCG
GAAACCCGATGGCTCGACTGAGCACGTTGGTCCCATCCTCGTGGGCTGGCTGCCCTGTCTCAGCTCCTAC
GACAGCTACTGCAGCAATCAAGTCGCCGCCAAAGCCCTGCTGGACCACAAAAAGCAGGACCACAGAGTGC
AGGACTTCCTCCAGCGCTGTTTAGAATCACCCTTCAGTCGCAAACTCGATCTCTGGAATTTCCTTGATAT
TCCCCGGAGCCGGCTAGTGAAGTATCCTCTGCTCCTTCGAGAAATCCTGAGGCATACACCAAATGATAAC
CCAGACCAGCAGCACCTGGAAGAAGCTATAAACATCATCCAGGGAATTGTGGCAGAAATCAACACCAAGA
CTGGGGAATCGGAATGCCGCTATTACAAAGAGCGGCTCCTTTACCTGGAAGAAGGCCAGAAAGATTCTCT
GATTGACAGCTCAAGGGTCCTGTGTTGTCATGGAGAGCTGAAAAATAACCGGGGTGTGAAACTCCATGTT
TTCCTGTTCCAAGAAGTGCTGGTGATCACCCGAGCTATCACTCACAACGAGCAGCTCTGCTATCAGTTGT
ACCGGCAGCCCATCCCTGTGAAGGACCTCACGCTGGAAGACCTGCAGGATGGAGAAGTGCGGCTGGGTGG
CTCCCTGCGCGGGGCCTTCAGCAACAACGAGAGAGTTAAAAACTTCTTCAGAGTCAGTTTCAAAAATGGA
TCCCAAAGTCAGACCCACTCACTACAAGCCAACGACACCTTCAACAAACAGCAGTGGCTCAACTGTATCC
GTCAGGCAAAAGAAACTGTTCTGTCTGCTGCCGGCCAGGCTGGCTTGCTTGACTCTGAGGGACTGGTCCA
GGGTCCCGGCACTGAGAACAGGGAGCCACAGGGAGAAACAAAACTTGAGCAGATGGACCAATCGGACAGC
GAGTCGGACTGCAGTATGGACACCAGTGAGGTCAGCCTTGAGTGTGAGCGGATGGAACAGACAGACGCTT
CCTGTGCAAACAGCAGGCCCGAGGAGAGTGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208408 representing BC005517
Red=Cloning site Green=Tags(s)

MVAKDYPFYLTVKRANCSLEAPLGSGVAKDEEPSNKRVKPLSRVTSLANLIPPVKTTPLKRFSQTLQRSI
SFRSESRPDILAPRAWSRNATSSSTKRRDSKLWSETFDVCVNQVLTAKEIKRQEAIFELSQGEEDLIEDL
KLAKKAYHDPMLKLSIMTEQELNQIFGTLDSLIPLHEELLSQLRDVRKPDGSTEHVGPILVGWLPCLSSY
DSYCSNQVAAKALLDHKKQDHRVQDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLLREILRHTPNDN
PDQQHLEEAINIIQGIVAEINTKTGESECRYYKERLLYLEEGQKDSLIDSSRVLCCHGELKNNRGVKLHV
FLFQEVLVITRAITHNEQLCYQLYRQPIPVKDLTLEDLQDGEVRLGGSLRGAFSNNERVKNFFRVSFKNG
SQSQTHSLQANDTFNKQQWLNCIRQAKETVLSAAGQAGLLDSEGLVQGPGTENREPQGETKLEQMDQSDS
ESDCSMDTSEVSLECERMEQTDASCANSRPEESV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC005517
ORF Size 1572 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC005517.1
RefSeq Size 3628 bp
RefSeq ORF 1574 bp
Locus ID 71704
Gene Summary Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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