Alpl (NM_007431) Mouse Tagged ORF Clone
CAT#: MG208392
- TrueORF®
Alpl (tGFP-tagged) - Mouse alkaline phosphatase 2, liver (Akp2)
ORF Plasmid: DDK
"NM_007431" in other vectors (3)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Ak; Akp; Akp-2; Akp2; ALP; APTNAP; T; TNAP; TNSALP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208392 representing NM_007431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTCACCATTTTTAGTACTGGCCATCGGCACCTGCCTTACCAACTCTTTTGTGCCAGAGAAAGAGA GAGACCCCAGTTACTGGCGACAGCAAGCCCAAGAGACCTTGAAAAATGCCCTGAAACTCCAAAAGCTCAA CACCAATGTAGCCAAGAATGTCATCATGTTCCTGGGAGATGGTATGGGCGTCTCCACAGTAACCGCTGCC CGAATCCTTAAGGGCCAGCTACACCACAACACGGGCGAGGAGACCCGGCTGGAGATGGACAAATTCCCCT TTGTGGCCCTCTCCAAGACATATAACACCAACGCTCAGGTCCCTGACAGCGCGGGCACTGCCACTGCCTA CTTGTGTGGCGTGAAGGCCAACGAGGGCACAGTGGGAGTGAGCGCAGCCACAGAGCGCACGCGATGCAAC ACCACTCAGGGCAATGAGGTCACATCCATCCTGCGCTGGGCCAAGGATGCTGGGAAGTCCGTGGGCATTG TGACTACCACTCGGGTGAACCACGCCACACCCAGTGCAGCCTACGCACACTCGGCCGATCGGGACTGGTA CTCGGATAACGAGATGCCACCAGAGGCTCTGAGCCAGGGCTGCAAGGACATCGCATATCAGCTAATGCAC AATATCAAGGATATCGACGTGATCATGGGTGGCGGCCGGAAATACATGTACCCGAAGAACAGAACTGATG TGGAATACGAACTGGATGAGAAGGCCAGGGGTACAAGGCTAGATGGCCTGGATCTCATCAGTATTTGGAA GAGCTTTAAACCCAGACACAAGCATTCCCACTATGTCTGGAACCGCACTGAACTGCTGGCCCTTGACCCC TCCAGGGTGGACTACCTCTTAGGTCTCTTTGAGCCCGGGGACATGCAGTATGAATTGAATCGGAACAACC TGACTGACCCTTCGCTCTCCGAGATGGTGGAGGTGGCCCTCCGGATCCTGACCAAAAACCTCAAAGGCTT CTTCTTGCTGGTGGAAGGAGGCAGGATTGACCACGGACATCATGAGGGTAAGGCCAAGCAGGCTCTGCAT GAAGCAGTGGAGATGGACCAGGCCATTGGCAAGGCAGGCGCCATGACATCCCAGAAAGACACCTTGACTG TGGTTACTGCTGATCATTCCCACGTTTTCACATTCGGTGGATACACCCCCCGGGGCAACTCCATCTTTGG TCTGGCTCCCATGGTGAGCGACACGGACAAGAAGCCCTTCACGGCCATCCTATATGGTAACGGGCCTGGC TACAAGGTGGTGGACGGTGAACGGGAAAATGTCTCCATGGTAGATTACGCTCACAACAACTACCAGGCCC AGTCCGCTGTTCCCCTGCGCCATGAGACCCACGGTGGAGAAGACGTGGCGGTCTTTGCCAAGGGCCCGAT GGCACACCTGCTTCACGGCGTCCATGAGCAGAACTACATTCCCCATGTGATGGCGTATGCCTCCTGCATT GGGGCCAACCTTGACCACTGTGCCTGGGCCGGCTCTGGGAGCGCACCCTCCCCAGGGGCCCTGCTGCTTC CACTGGCTGTGCTCTCCCTACGCACCCTGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208392 representing NM_007431
Red=Cloning site Green=Tags(s) MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAA RILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCN TTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMH NIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDP SRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALH EAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPG YKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCI GANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007431 |
ORF Size | 1572 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007431.3 |
RefSeq Size | 2459 bp |
RefSeq ORF | 1575 bp |
Locus ID | 11647 |
UniProt ID | P09242 |
Gene Summary | This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR208392 | Alpl (Myc-DDK-tagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl) |
CNY 3,912.00 |
|
MR208392L3 | Lenti ORF clone of Alpl (Myc-DDK-tagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl) |
CNY 6,460.00 |
|
MR208392L4 | Lenti ORF clone of Alpl (mGFP-tagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl) |
CNY 6,312.00 |