Gtf2h1 (BC052837) Mouse Tagged ORF Clone

CAT#: MG208340

  • TrueORF®

Gtf2h1 (tGFP-tagged) - Mouse general transcription factor II H, polypeptide 1 (cDNA clone MGC:60648 IMAGE:30049274)



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Mouse Protein / Lysate Request

CNY 5,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms p62, 62kDa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208340 representing BC052837
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGAGAATTGCTTGGGCACCTGAAGGCAAAGACAGATTTACAATCAGCCATATGTATGCAGATA
TTAAATGCCAGAAAATCAGTCCAGAGGGAAAAGCTAAAATACAACTTCAGCTGGTCCTGCATGCAGGGGA
CACAACAAACTTCCATTTTTCCAACGAAAGCACGGCAGTGAAAGAACGGGATGCAGTGAAGGACCTCCTT
CAGCAGCTGCTGCCCAAGTTCAAGCGGAAAGCTAATAAAGAGCTGGAGGAGAAGAACAGAATGCTCCAAG
AAGATCCTGTTTTATTCCAACTCTATAAAGACCTTGTTGTGAGCCAAGTGATCAGTGCTGAGGAATTCTG
GGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTACATCCAGTCACAAGCAGGATGTTGGTATTTCT
GCAGCATTTCTGGCTGATGTCCGGCCCCAAACGGATGGCTGTAATGGTCTGAGATATAATTTAACTTCTG
ATATCATTGAATCTATATTTAGGACCTATCCAGCAGTAAAAATGAAATATGCAGAAACTGTACCACACAA
CATGACAGAAAAGGAGTTCTGGACACGCTTTTTCCAGTCCCATTATTTTCACAGGGACCGACTAAATACA
GGATCGAAGGACCTCTTTGCAGAATGTGCCAAAATAGATGAGAAGGGATTAAAAACAATGGTTTCATTAG
GAGTGAAAAACCCAATGCTAGATTTGACATCGTTGGAAGATAAGCCATTAGACGAGGGCTATGGCATTTC
CTCTGTGCCATCTACTTCCAATTCCAAATCCATAAAGGAGAATAGTAATGCTGCCATCATCAAAAGGTTT
AACCACCACAGTGCCATGGTCCTGGCTGCTGGTCTCAGGAAACAACAAGCACAAAATGGACAGAATGGTG
AGCCCAGCAGCGTGGATGGGAATTCTGGGGATACAGACTGCTTTCAGCCAGCAGTCAAAAGGGCAAAGTT
GCAGGAGTCCATTGAATATGAAGACTTGGGAAACAACAATTCTGTGAAAACGATTGCACTGAATCTCAAG
AAGTCAGACAGGTATTACCATGGTCCTACTCCAATTCAGTCACTGCAGTATGCAACAAGTCAGGACATTA
TTAACTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTACACACCCAAGTTAACTCAGGTTCTCTCAAG
CAGTGCTGCTAGTAGCACCATCACAGCCCTGTCACCTGGAGGAGCTCTTATGCAGGGAGGGACACAGCAA
GCCGTAAACCAGATGGTGCCAAACGATATTCAGTCTGAATTGAAACACTTGTATGTGGCTGTTGGGGAAC
TTCTGCGGCATTTTTGGTCTTGCTTTCCTGTTAATACACCATTCCTAGAAGAAAAGGTAGTGAAAATGAA
GAGTAATTTGGAACGGTTCCAAGTTACAAAGCTCTGCCCATTCCAAGAAAAGATTCGGAGGCAGTATTTA
AGCACAAATCTGGTAAGTCACATAGAAGAGATGCTGCAGACAGCCTACAACAAGCTCCACGCGTGGCAAT
CCCGGCGCCTGATGAAGAAGACG


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208340 representing BC052837
Red=Cloning site Green=Tags(s)

MAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLL
QQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGIS
AAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNT
GSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRF
NHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLK
KSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQ
AVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYL
STNLVSHIEEMLQTAYNKLHAWQSRRLMKKT

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052837
ORF Size 1565 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052837, AAH52837
RefSeq Size 2203 bp
RefSeq ORF 1565 bp
Locus ID 14884
Gene Summary Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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