Gtf2h1 (BC052837) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	p62, 62kDa
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208340 representing BC052837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGAGAATTGCTTGGGCACCTGAAGGCAAAGACAGATTTACAATCAGCCATATGTATGCAGATA TTAAATGCCAGAAAATCAGTCCAGAGGGAAAAGCTAAAATACAACTTCAGCTGGTCCTGCATGCAGGGGA CACAACAAACTTCCATTTTTCCAACGAAAGCACGGCAGTGAAAGAACGGGATGCAGTGAAGGACCTCCTT CAGCAGCTGCTGCCCAAGTTCAAGCGGAAAGCTAATAAAGAGCTGGAGGAGAAGAACAGAATGCTCCAAG AAGATCCTGTTTTATTCCAACTCTATAAAGACCTTGTTGTGAGCCAAGTGATCAGTGCTGAGGAATTCTG GGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTACATCCAGTCACAAGCAGGATGTTGGTATTTCT GCAGCATTTCTGGCTGATGTCCGGCCCCAAACGGATGGCTGTAATGGTCTGAGATATAATTTAACTTCTG ATATCATTGAATCTATATTTAGGACCTATCCAGCAGTAAAAATGAAATATGCAGAAACTGTACCACACAA CATGACAGAAAAGGAGTTCTGGACACGCTTTTTCCAGTCCCATTATTTTCACAGGGACCGACTAAATACA GGATCGAAGGACCTCTTTGCAGAATGTGCCAAAATAGATGAGAAGGGATTAAAAACAATGGTTTCATTAG GAGTGAAAAACCCAATGCTAGATTTGACATCGTTGGAAGATAAGCCATTAGACGAGGGCTATGGCATTTC CTCTGTGCCATCTACTTCCAATTCCAAATCCATAAAGGAGAATAGTAATGCTGCCATCATCAAAAGGTTT AACCACCACAGTGCCATGGTCCTGGCTGCTGGTCTCAGGAAACAACAAGCACAAAATGGACAGAATGGTG AGCCCAGCAGCGTGGATGGGAATTCTGGGGATACAGACTGCTTTCAGCCAGCAGTCAAAAGGGCAAAGTT GCAGGAGTCCATTGAATATGAAGACTTGGGAAACAACAATTCTGTGAAAACGATTGCACTGAATCTCAAG AAGTCAGACAGGTATTACCATGGTCCTACTCCAATTCAGTCACTGCAGTATGCAACAAGTCAGGACATTA TTAACTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTACACACCCAAGTTAACTCAGGTTCTCTCAAG CAGTGCTGCTAGTAGCACCATCACAGCCCTGTCACCTGGAGGAGCTCTTATGCAGGGAGGGACACAGCAA GCCGTAAACCAGATGGTGCCAAACGATATTCAGTCTGAATTGAAACACTTGTATGTGGCTGTTGGGGAAC TTCTGCGGCATTTTTGGTCTTGCTTTCCTGTTAATACACCATTCCTAGAAGAAAAGGTAGTGAAAATGAA GAGTAATTTGGAACGGTTCCAAGTTACAAAGCTCTGCCCATTCCAAGAAAAGATTCGGAGGCAGTATTTA AGCACAAATCTGGTAAGTCACATAGAAGAGATGCTGCAGACAGCCTACAACAAGCTCCACGCGTGGCAAT CCCGGCGCCTGATGAAGAAGACG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208340 representing BC052837 Red=Cloning site Green=Tags(s) MAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLL QQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSTSSHKQDVGIS AAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAETVPHNMTEKEFWTRFFQSHYFHRDRLNT GSKDLFAECAKIDEKGLKTMVSLGVKNPMLDLTSLEDKPLDEGYGISSVPSTSNSKSIKENSNAAIIKRF NHHSAMVLAAGLRKQQAQNGQNGEPSSVDGNSGDTDCFQPAVKRAKLQESIEYEDLGNNNSVKTIALNLK KSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQ AVNQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYL STNLVSHIEEMLQTAYNKLHAWQSRRLMKKT SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052837
ORF Size	1565 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052837, AAH52837
RefSeq Size	2203 bp
RefSeq ORF	1565 bp
Locus ID	14884
Gene Summary	Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]

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