Rcc2 (BC086666) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA1470, Td60
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208331 representing BC086666 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGAAGAAGGGGGCGGCCTGGGAGGAGCCGAGTTCGGGCAACGGCACGGCGCGCGCGGGGCCCC GGCGACGCGGCGGCCCCGCGGGCAGGAAGCGCGAGCGGCCCGAGCGCTGCAGCAGCAGCAGCGGCGGCGG CAGCAGCGGCGACGAGGACGGCCCGGAGCTGGACGGGGCCCCCGGCGGCGGCAAGCGCACAGCCAGGCCG GCGACGGCGGGCAAGGCGGCCGGCGCGGCCGCGATCATCACGGAGCCCGAGCACACGAAGGAGCGCGTCA AACTTGAAGGGTCTAAGTGCAAAGGGCAGCTTTTGATTTTTGGGGCAACCAACTGGGACTTGATTGGACG GAAGGAAGTGCCTAAACAGCAAGCTGCGTACCGCAACCTTGGTCAGAATTTGTGGGGGCCCCATAGGTAT GGGTGCCTGTCAGGAGTCCGGGTGCGAACCGTGGTTTCAGGCTCATGTGCTGCCCACAGCCTCCTCATCA CCACAGAAGGGAAGCTGTGGAGCTGGGGTCGGAATGAGAAGGGGCAGCTGGGCCATGGTGATACCAAGAG GGTGGAAGCCCCCAGACTCATCGAGGCCCTCAGCCATGAGGCAATCGTGCTGGCAGCCTGTGGGCGCAAC CACACCCTGGCCTTGACAGATACTGGATCAGTGTTTGCCTTCGGAGAGAATAAAATGGGGCAGCTGGGTC TCGGGAACCAGACAGATGCCGTCCCAAGTCCTGCTCAGATAATGTACAACGGGCAGCCAATCACCAAGAT GGCCTGCGGGGCCGAGTTCAGCATGCTGATGGACTGTAAAGGAAACCTCTACTCCTTTGGGTGCCCTGAG TATGGCCAGCTGGGGCACAACTCAGATGGCAAGTTCATCGCCCGGGCGCAGAGGATAGAATATGATTGTG AGCTGGTGCCCCGCAGAGTGGCCATCTTCATCGAGAAGACCAAGGACGGGCAGATCTTACCTGTCCCCAA CGTGGTGGTTCGAGATGTAGCCTGTGGCGCTAACCACACACTGGTCCTGGACTCTCAGAAGCGAGTGTTC TCCTGGGGCTTTGGTGGCTATGGCCGGCTGGGCCATGCCGAGCAGAAGGATGAGATGGTGCCTCGCCTGG TGAAGCTGTTCGACTTCCCGGGGCGTGGTGCAACCCAGATCTATGCCGGCTACACCTGCTCCTTCGCTGT CAGTGAAGTGGGTGGCCTGTTTTTCTGGGGGGCCACCAATACCTCCCGAGAGTCTACCATGTACCCGAAG GCAGTGCAGGACCTCTGCGGCTGGCGGATCCGGAGCCTTGCCTGCGGGAAGAGCAGCATCATCGTAGCTG CGGACGAGAGTACTATCAGTTGGGGTCCATCGCCAACCTTCGGGGAATTGGGGTATGGGGACCACAAGCC CAAGTCTTCTACTGCGGCCCAGGAGGTGAAGACTCTGGATGGCATCTTCTCCGAACAGGTGGCCATGGGC TATTCACACTCCTTGGTGATAGCGCGAGACGAGAGCGAGGCTGAGAAGGAGAAGCTGCAGAGGCTACCTG AGTACACCCCGCGCACCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208331 representing BC086666 Red=Cloning site Green=Tags(s) MPRKKGAAWEEPSSGNGTARAGPRRRGGPAGRKRERPERCSSSSGGGSSGDEDGPELDGAPGGGKRTARP ATAGKAAGAAAIITEPEHTKERVKLEGSKCKGQLLIFGATNWDLIGRKEVPKQQAAYRNLGQNLWGPHRY GCLSGVRVRTVVSGSCAAHSLLITTEGKLWSWGRNEKGQLGHGDTKRVEAPRLIEALSHEAIVLAACGRN HTLALTDTGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMLMDCKGNLYSFGCPE YGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVF SWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGATQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPK AVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMG YSHSLVIARDESEAEKEKLQRLPEYTPRTL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC086666
ORF Size	1560 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC086666, AAH86666
RefSeq Size	1976 bp
RefSeq ORF	1562 bp
Locus ID	108911
Gene Summary	Multifunctional protein that may effect its functions by regulating the activity of small GTPases, such as RAC1 and RALA. Required for normal progress through the cell cycle, both during interphase and during mitosis. Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles. Required for normal organization of the microtubule cytoskeleton in interphase cells. Functions as guanine nucleotide exchange factor (GEF) for RALA. Interferes with the activation of RAC1 by guanine nucleotide exchange factors (By similarity). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed:25074804). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]

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