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Ralgps1 (BC072656) Mouse Tagged ORF Clone
CAT＃: MG208271
- TrueORF®
Ralgps1 (tGFP-tagged) - Mouse Ral GEF with PH domain and SH3 binding motif 1 (cDNA clone MGC:99991 IMAGE:30536315)
ORF Plasmid: DDK
"BC072656" in other vectors (4)
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CNY 3,230.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA0351, RALGEF2, RALGPS1A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208271 representing BC072656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAAGAGGAATGGTCTGATGGCTAGCGTGTTGGTCACCTCTGCCACTCCACAGGGCAGCAGCAGCT CAGACTCGCTGGAGGGCCAGAGCTGCGACTATGCCAGCAAGAGCTACGATGCTGTCGTCTTCGACGTTCT GAAAGTGACTCCCGAGGAGTTTGCCAGTCAGATCACATTGATGGACATCCCCGTGTTCAAAGCCATCCAG CCAGAGGAGTTGGCCAGTTGTGGATGGAGTAAAAAGGAGAAACACAGTCTTGCCCCCAACGTCGTGGCCT TTACCCGGAGGTTCAACCAGGTCAGTTTTTGGGTCGTACGAGAAATTCTAACAGCACAGACTTTAAAAAT AAGGGCAGAAATCCTGAGCCATTTTGTGAAAATAGCCAAGAAACTTCTAGAACTGAACAACCTTCATTCT CTCATGTCTGTGGTGTCAGCATTACAAAGTGCTCCCATCTTCAGGCTGACAAAAACCTGGGCTCCTTTGA ATCGAAAAGACAAGACTACTTTTGAGAAACTTGACTACCTGATGTCCAAAGAAGACAACTACAAGCGAAC CCGGGACTACATCCGAAGTCTGAAGATGGTGCCCAGCATTCCCTATCTAGGAATCTACCTTCTGGACTTG ATCTACATTGATTCTGCATACCCTGCCTCTGGCAGCATCATGGAAAACGAACAACGATCCAACCAGATGA ACAACATCCTCCGAATCATTGCCGATTTACAAGTTTCCTGTAGCTATGATCACCTCACTACCCTGCCACA TGTGCAAAAGTACCTGAAGTCTGTGCGCTACATCGAGGAGCTCCAGAAGTTTGTAGAAGATGATAATTAC AAGTTGTCACTCAGAATCGAACCAGGAAGCAGCTCTCCAAGACTAGTTTCGTCCAAGGAAGATCTTGCAG GCCCCTCTGCCGGCTCCAGCTCAGCCAGGTTCCGCCGGAGGCCCACCTGTCCAGATACATCTGTGGCCGG CAGCCTCCCCACACCGCCAGTCCCCAGACACAGGAAGAGCCACAGCCTAGGCAACAACAGGGGACGTCTT TATGCCACGCTGGGACCTAACTGGCGGGTTCCAGTTCGGAATTCTCCCAGAACTCGGAGCTGTGTCTACA GCCCCACGAGCCCTTGCACCTGTACTGTGGGAAGCTCAGCCACTGTGCCCACTATGGAGGGGCCTCTGAG ACGGAAAACCCTGCTCAAGGAAGGCCGGAAGCCTGCACTGTCCTCGTGGACCAGGTACTGGGTTGTACTC TCAGGAGCCACCCTCCTTTACTATGGAGCCAAGTCTTTGCGGGGTACAGACAGAAAACATTATAAATCTA CACCTGGCAAGAAGGTCTCCATCGTAGGCTGGATGGTGCAGCTGCCAGATGACCCCGAGCACCCAGATAT CTTCCAGCTGAATAACCCTGACAAAGGCAATGTTTACAAGTTCCAGACGGGCTCCCGTTTCCATGCGATA CTGTGGCACAAGCATTTGGATGATGCATGTAAAAGCAGCAGGCCTCAGGTACCTGCAAACCTTATGTCCT TTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208271 representing BC072656
Red=Cloning site Green=Tags(s) MYKRNGLMASVLVTSATPQGSSSSDSLEGQSCDYASKSYDAVVFDVLKVTPEEFASQITLMDIPVFKAIQ PEELASCGWSKKEKHSLAPNVVAFTRRFNQVSFWVVREILTAQTLKIRAEILSHFVKIAKKLLELNNLHS LMSVVSALQSAPIFRLTKTWAPLNRKDKTTFEKLDYLMSKEDNYKRTRDYIRSLKMVPSIPYLGIYLLDL IYIDSAYPASGSIMENEQRSNQMNNILRIIADLQVSCSYDHLTTLPHVQKYLKSVRYIEELQKFVEDDNY KLSLRIEPGSSSPRLVSSKEDLAGPSAGSSSARFRRRPTCPDTSVAGSLPTPPVPRHRKSHSLGNNRGRL YATLGPNWRVPVRNSPRTRSCVYSPTSPCTCTVGSSATVPTMEGPLRRKTLLKEGRKPALSSWTRYWVVL SGATLLYYGAKSLRGTDRKHYKSTPGKKVSIVGWMVQLPDDPEHPDIFQLNNPDKGNVYKFQTGSRFHAI LWHKHLDDACKSSRPQVPANLMSFE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC072656 |
| ORF Size | 1547 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC072656, AAH72656 |
| RefSeq Size | 6066 bp |
| RefSeq ORF | 1547 bp |
| Locus ID | 241308 |
| Gene Summary | Guanine nucleotide exchange factor for the small GTPase RALA. May be involved in cytoskeleton organization.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218644 | Ralgps1 (untagged) - Mouse Ral GEF with PH domain and SH3 binding motif 1 (cDNA clone MGC:99991 IMAGE:30536315), (10ug) |
CNY 6,940.00 |
|
| MR208271 | Ralgps1 (Myc-DDK-tagged) - Mouse Ral GEF with PH domain and SH3 binding motif 1 (cDNA clone MGC:99991 IMAGE:30536315) |
CNY 3,832.00 |
|
| MR208271L1 | Lenti ORF clone of Ralgps1 (Myc-DDK-tagged) - Mouse Ral GEF with PH domain and SH3 binding motif 1 (cDNA clone MGC:99991 IMAGE:30536315) |
CNY 4,850.00 |
|
| MR208271L2 | Lenti ORF clone of Ralgps1 (mGFP-tagged) - Mouse Ral GEF with PH domain and SH3 binding motif 1 (cDNA clone MGC:99991 IMAGE:30536315) |
CNY 4,850.00 |
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