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Sf3a3 (BC092058) Mouse Tagged ORF Clone
CAT＃: MG208035
- TrueORF®
Sf3a3 (tGFP-tagged) - Mouse splicing factor 3a, subunit 3 (cDNA clone MGC:103282 IMAGE:4017388)
ORF Plasmid: DDK
"BC092058" in other vectors (3)
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CN¥ 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 60kDa; 4930512K19Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208035 representing BC092058
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACAATTTTAGAGCAGCAACGGCGCTATCATGAGGAGAAGGAACGGCTTATGGATGTTATGGCCA AAGAGATGCTTACGAAGAAGTCCACGCTTCGGGACCAGATCAATTCCGATCACCGCACTCGGGCCATGCA AGATAGGTACATGGAGGTCAGTGGGAACCTGAGGGATTTGTATGATGATAAGGACGGACTGCGGAAGGAG GAGCTCAATGCTATCTCAGGACCCAACGAGTTTGCTGAATTCTATAACAGACTCAAGCAGATAAAGGAAT TCCATCGGAAGCACCCAAATGAGATCTGTGTGCCAATGTCAGTGGAATTTGAGGAGCTGCTGAAGGCTCG AGAGAATCCAAGTGAAGAGGCACAAAACTTGGTGGAGTTCACAGATGAAGAGGGATATGGTCGCTACCTT GATCTCCATGACTGCTACCTCAAATACATTAACCTGAAGGCATCTGAGAAGCTGGATTACATCACATACC TGTCCATCTTTGACCAGTTGTTTGACATTCCTAAAGAAAGAAAGAATGCAGAGTACAAAAGATACCTGGA GATGCTGCTGGAGTACCTACAGGATTACACAGATAGAGTGAAGCCCCTTCAGGATCAGAACGAACTCTTT GGGAAGATTCAGACCGACTTTGAGAAGAAGTGGGATAATGGCACCTTTCCTGGATGGCCGAAAGAGACAA GCAGCGCCCTGACTCACGCGGGAGCCCATCTTGATCTCTCCGCATTCTCCTCCTGGGAGGAGTTGGCATC CCTGGGTCTGGATAGGTTGAAATCTGCACTATTGGCATTGGGCCTGAAGTGTGGCGGGACCCTAGAAGAA CGAGCTCAGAGGCTCTTCAGCACCAAAGGAAAGTCCTTGGAGTCACTCGACACTTCTCTGTTTGCCAAAA ATCCCAAGTCAAAGGGCACAAAAAGAGACACTGAGAGGAACAAAGACATTGCTTTCCTGGAAGCCCAGAT CTATGAATACGTGGAGATTCTCGGGGAACAGCGACAGCTCACTCATGAAAATGTACAGCGCAAGCAAGCC AGGACAGGCGAGGAGCGGGAGGAGGAGGAGGAGGAGCAGATCAGCGAGAGCGAAAGTGAGGACGAAGAGA ATGAGATCATTTACAACCCCAAGAACCTGCCGCTGGGCTGGGACGGCAAGCCCATCCCCTACTGGCTGTA CAAGCTCCACGGCCTGAATATCAACTACAACTGTGAGATCTGTGGGAACTACACCTACCGAGGGCCCAAG GCCTTCCAGCGGCACTTTGCGGAGTGGCGTCATGCTCATGGCATGAGGTGTTTGGGCATCCCAAACACTG CCCACTTTGCAAATGTGACACAGATTGAAGATGCTGTCTCCTTGTGGGCCAAGCTGAAACTGCAGAAGGC TTCAGAACGGTGGCAGCCTGACACCGAGGAAGAATATGAAGACTCCAGTGGAAATGTTGTGAACAAGAAG ACATACGAGGATCTGAAAAGACAAGGGCTGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208035 representing BC092058
Red=Cloning site Green=Tags(s) METILEQQRRYHEEKERLMDVMAKEMLTKKSTLRDQINSDHRTRAMQDRYMEVSGNLRDLYDDKDGLRKE ELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFEELLKARENPSEEAQNLVEFTDEEGYGRYL DLHDCYLKYINLKASEKLDYITYLSIFDQLFDIPKERKNAEYKRYLEMLLEYLQDYTDRVKPLQDQNELF GKIQTDFEKKWDNGTFPGWPKETSSALTHAGAHLDLSAFSSWEELASLGLDRLKSALLALGLKCGGTLEE RAQRLFSTKGKSLESLDTSLFAKNPKSKGTKRDTERNKDIAFLEAQIYEYVEILGEQRQLTHENVQRKQA RTGEEREEEEEEQISESESEDEENEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPK AFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLKLQKASERWQPDTEEEYEDSSGNVVNKK TYEDLKRQGLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC092058 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC092058, AAH92058 |
| RefSeq Size | 1777 bp |
| RefSeq ORF | 1505 bp |
| Locus ID | 75062 |
| Gene Summary | Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208035 | Sf3a3 (Myc-DDK-tagged) - Mouse splicing factor 3a, subunit 3 (cDNA clone MGC:103282 IMAGE:4017388) |
CN¥ 3,728.00 |
|
| MR208035L3 | Lenti ORF clone of Sf3a3 (Myc-DDK-tagged) - Mouse splicing factor 3a, subunit 3 (cDNA clone MGC:103282 IMAGE:4017388) |
CN¥ 4,750.00 |
|
| MR208035L4 | Lenti ORF clone of Sf3a3 (mGFP-tagged) - Mouse splicing factor 3a, subunit 3 (cDNA clone MGC:103282 IMAGE:4017388) |
CN¥ 4,750.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
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