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Pctk1 (BC011069) Mouse Tagged ORF Clone
CAT＃: MG207961
- TrueORF®
Pctk1 (tGFP-tagged) - Mouse PCTAIRE-motif protein kinase 1 (cDNA clone MGC:18746 IMAGE:4006745)
ORF Plasmid: DDK
"BC011069" in other vectors (4)
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CN¥ 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Crk5; Pctaire1; Pctk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207961 representing BC011069
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACTCCGAGGGGGCCGAGGCATAGATAAGA CCAATGGTGTCCCTGAGCAGATAGGCCTGGATGAGAGTGGTGGCGGTGGTGGCAGTGACCTTGGAGAAGC TCCCACCCGTATTGCCCCTGGGGAGCTTCGCTCTGTTCGGGGCCCACTCAGCTCTGCACCAGAGATTGTG CACGAGGACATGAAGATGGGATCTGATGGGGAGAGTGACCAGGCTTCAGCCACATCCTCAGATGAGGTGC AGTCTCCAGTGAGAGTGCGCATGCGCAACCACCCCCCACGCAAGATCTCCACTGAGGACATCAACAAACG CCTGTCACTACCAGCTGACATACGGCTGCCTGAGGGCTACCTTGAGAAGCTGACCCTCAATAGCCCCATC TTTGATAAGCCTCTTAGCCGGCGCCTCCGCCGAGTCAGTTTGTCTGAGATTGGCTTTGGAAAACTGGAGA CCTACATCAAGCTAGACAAGCTGGGTGAGGGTACCTATGCCACTGTCTACAAAGGCAAAAGCAAGCTCAC AGACAACCTTGTAGCACTTAAGGAGATCAGACTGGAACACGAAGAAGGGGCACCCTGTACTGCTATCCGG GAAGTATCCCTGCTTAAGGACCTCAAGCATGCCAACATCGTCACACTACATGACATTATCCACACAGAGA AGTCCCTCACACTTGTCTTTGAATACTTGGACAAGGACCTGAAGCAGTACCTGGATGACTGTGGAAATGT CATCAACATGCACAATGTGAAACTGTTCCTGTTCCAGTTGCTCCGTGGCCTGGCCTACTGCCACAGGCAG AAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTACTCATCAACGAGAGGGGAGAGCTCAAACTGGCAG ATTTTGGCCTGGCTCGTGCCAAGTCAATTCCTACTAAAACATACTCCAACGAAGTGGTGACACTGTGGTA CCGGCCCCCTGACATCCTACTTGGGTCCACAGACTACTCTACCCAAATTGACATGTGGGGTGTAGGCTGC ATCTTCTATGAGATGGCCACCGGCCGGCCCCTCTTCCCAGGCTCCACAGTGGAAGAACAGCTGCACTTCA TCTTCCGCATTTTGGGAACCCCAACTGAGGAGACATGGCCAGGTATCCTGTCCAATGAGGAGTTTAGAAC ATACAACTACCCCAAGTACCGAGCCGAGGCCCTTCTGAGCCATGCACCCCGACTTGATAGCGATGGAGCT GACCTCCTCACCAAGCTGCTGCAGTTTGAGGGTCGCAATCGGATCTCTGCTGAGGATGCCAGGAAACATC CATTCTTTCTCAGCTTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGCACTAAAGGA GGTACAGCTACAAAAGGAGGCCAACATTCGGTCCACTTCTATGCCTGACTCAGGCAGGCCAGCTTTCCGT GTGGTGGATACCGAGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207961 representing BC011069
Red=Cloning site Green=Tags(s) MDRMKKIKRQLSMTLRGGRGIDKTNGVPEQIGLDESGGGGGSDLGEAPTRIAPGELRSVRGPLSSAPEIV HEDMKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPI FDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIR EVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNVINMHNVKLFLFQLLRGLAYCHRQ KVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGC IFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFRTYNYPKYRAEALLSHAPRLDSDGA DLLTKLLQFEGRNRISAEDARKHPFFLSLGERIHKLPDTTSIFALKEVQLQKEANIRSTSMPDSGRPAFR VVDTEF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011069 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011069, AAH11069 |
| RefSeq Size | 2815 bp |
| RefSeq ORF | 1490 bp |
| Locus ID | 18555 |
| Gene Summary | Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217728 | Pctk1 (untagged) - Mouse PCTAIRE-motif protein kinase 1 (cDNA clone MGC:18746 IMAGE:4006745), (10ug) |
CN¥ 6,940.00 |
|
| MR207961 | Pctk1 (Myc-DDK-tagged) - Mouse PCTAIRE-motif protein kinase 1 (cDNA clone MGC:18746 IMAGE:4006745) |
CN¥ 3,656.00 |
|
| MR207961L3 | Lenti ORF clone of Pctk1 (Myc-DDK-tagged) - Mouse PCTAIRE-motif protein kinase 1 (cDNA clone MGC:18746 IMAGE:4006745) |
CN¥ 4,750.00 |
|
| MR207961L4 | Lenti ORF clone of Pctk1 (mGFP-tagged) - Mouse PCTAIRE-motif protein kinase 1 (cDNA clone MGC:18746 IMAGE:4006745) |
CN¥ 4,750.00 |
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