Cacnb4 (BC026479) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC31529, lethargic
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207780 representing BC026479 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACAATTTGTACCTGCATGGAGTTGAAGACTCGGAGGCTGGGTCAGCAGATTCCTATACAAGCA GGCCATCGGACTCCGATGTCTCCTTGGAAGAGGACCGGGAAGCGATCCGGCAAGAGCGAGAACAGCAAGC AGCTATCCAGCTTGAGAGAGCGAAGTCCAAACCTGTAGCTTTTGCGGTGAAAACGAACGTGAGCTACTGT GGTGCCCTGGACGAAGATGTGCCCGTTCCCAGCACAGCCATCTCCTTTGACGCCAAGGACTTTCTTCACA TTAAAGAGAAATATAACAATGATTGGTGGATAGGAAGACTGGTAAAAGAGGGCTGTGAGATTGGCTTCAT CCCAAGTCCACTGCGCTTGGAGAATATTCGGATTCAACAGGAACAGAAAAGAGGCCGTTTTCATGGCGGG AAATCGAGTGGAAACTCTTCCTCCAGTCTGGGAGAAATGGTATCAGGAACATTCCGAGCAACTCCCACAA CAACAGCAAAACAGAAGCAGAAAGTGACGGAGCACATTCCTCCGTATGACGTCGTGCCGTCAATGCGTCC TGTGGTGTTAGTGGGGCCATCACTGAAAGGTTATGAGGTAACAGACATGATGCAGAAAGCCCTCTTTGAT TTCCTGAAGCACAGGTTTGATGGGAGGATATCAATAACAAGAGTGACAGCTGACATTTCTCTTGCTAAGA GATCTGTCCTCAACAATCCTAGCAAGAGAGCAATAATTGAACGTTCCAACACCAGATCCAGCTTAGCGGA AGTACAAAGTGAAATTGAAAGAATTTTTGAGTTGGCAAGATCTTTGCAATTGGTTGTTCTTGATGCAGAC ACCATCAACCACCCAGCACAGCTGATAAAGACATCCTTAGCACCCATCATCGTCCACGTGAAGGTCTCGT CCCCAAAGGTTTTACAGCGGCTGATTAAGTCCAGAGGAAAGTCCCAAAGCAAACACTTGAATGTTCAACT GGTGGCGGCCGATAAACTGGCCCAGTGCCCGCCTGAAATGTTTGATGTTATATTAGATGAGAATCAACTT GAGGATGCCTGTGAACATCTGGGAGAGTACCTGGAGGCATACTGGCGTGCCACCCACACGAGCAGTAGCA CCCCTATGACCCCATTACTGGGGCGGAACGTGGGCTCCACAGCCCTCTCACCATATCCCACAGCAATCTC TGGATTACAGAGTCAGCGAATGAGACACAGCAACCATTCTACAGAGAATTCTCCAATTGAAAGACGAAGC CTAATGACCTCGGATGAAAATTACCACAATGAGAGGGCCCGCAAGAGTAGGAACCGCTTGTCTTCCAGCT CCCAGCACAGCCGAGACCACTACCCTCTGGTGGAAGAAGATTACCCGGACTCGTACCAGGACACTTATAA GCCCCATAGGAACCGAGGATCGCCCGGGGGGTGCAGCCATGACTCCCGACATAGGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207780 representing BC026479 Red=Cloning site Green=Tags(s) MYDNLYLHGVEDSEAGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYC GALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGG KSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFD FLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDAD TINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQL EDACEHLGEYLEAYWRATHTSSSTPMTPLLGRNVGSTALSPYPTAISGLQSQRMRHSNHSTENSPIERRS LMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGSPGGCSHDSRHRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026479
ORF Size	1460 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026479, AAH26479
RefSeq Size	2188 bp
RefSeq ORF	1460 bp
Locus ID	12298
Gene Summary	The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function]

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