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Trim11 (BC020102) Mouse Tagged ORF Clone
CAT＃: MG207741
- TrueORF®
Trim11 (tGFP-tagged) - Mouse tripartite motif protein 11 (cDNA clone MGC:28318 IMAGE:4014766)
ORF Plasmid: DDK
"BC020102" in other vectors (4)
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CNY 3,040.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207741 representing BC020102
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCCAGACTTGTCCACCAACCTCCAGGAGGAGGCCACCTGCGCCATCTGCCTGGATTACTTCA CCGACCCGGTGATGACCGACTGCGGCCACAACTTCTGCCGCGAGTGCATCCGACGTTGCTGGGGCCAGCC CGAGGGCCCATACGCGTGCCCCGAGTGTCGCGAGCTGTCGGCGCAGAGGAACCTACGGCCCAACCGCCCG CTCGCCAAAATGGCAGAAATGGCACGACGCCTGCACCCGCCTTCTCCGGTCCCGCAGGGCGTCTGTGCGG CGCACCGCGAGCCGCTGACCACCTTCTGCGGCGACGACCTCAGCCTGCTGTGCCCCATCTGCGAGCGTTC GGAGCACTGGACTCACCGGGTGAGACCGCTGCAGGAGGCGGCGGACGACCTCAAGGGGAGGCTGGAGAAG TCACTGGAGCACCTACGGAAACAAATGGAGGACGCAATGCTGTTCCAAGCCCAGGCTGAGGAAACCTGTG CCTTGTGGCAGAAGATGGTGGAGAGCCAGCGGCAGAATGTGCTGGGCGAGTTTGAGAGGCTGCGCCGCCT GTTGGCAGAGGAGGAGCAACAGCTGCTGCAGAAGCTGGAGGAGGAAGAGCTGGAGGTTCTGCCGCGCCTG CGTGAAGGCGCTGCGAGGCTTGGCCAGCAAAGCACGCAGCTGGCAGCCCTCATCTCCGAGCTTGAGAGCC GCTGCCAGCTGCCAGCCCTGGGTTTACTGCAGCTCTGCATTGAGTGCTGCGCCCTGGAGAGAGAAGCGTC TATAGCCAAGGACATTAAGGACGCCCTGTGCAGGGTGCAGGATGTGAAGCTGCAGCCTCCAGCAGTGGTG CCCATGGAGCTGAGGACCGTGTGCCGGGTCCCAGGGCTGGTGGAGACCCTGCGGAGGTTCCGAGGGGACA TAACCCTGGACCCAGACACCGCCAACCCTGAGCTGGTCTTATCTGAGGACCGGAGGAGTGTGCAGCGTGG TGAACAGCGGCAGGCCCTGCCTGACAACCCAGAGCGGTTCGACCCTGGCCCTTGTGTGCTGGGCCAGGAG CGCATTACCTCTGGCCGCCACTACTGGGAGGTGGAAGTCGGGGACCAGACCAGCTGGGCACTCGGCGTGT GTAAAGAAACTGCCAACAGGAAGGAGAAGGGGGAGCTGTCGGCTGGCAATGGGTTCTGGATCCTGGTGTT CCTGGGGAGTTTCTATAATTCCAATGAGCCGGCCTTCTCCCCACTGCGGGACCCTCCCAAGCGTGTGGGG ATTTTTCTGGACTATGAAGCTGGCCATCTCTCATTCTACAGTGCCACGGATGGGTCGCTGCTGTTTATCT TCCCCGAGACCCTGTTCTCAGGGACACTCCGGCCCCTCTTCTCACCTCTGTCAAGCAGCCCAACCCCTAT GACTATCTGCAGGCTGATAGGTGTATCTGGGGACACCCTTGGTCCGCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207741 representing BC020102
Red=Cloning site Green=Tags(s) MAAPDLSTNLQEEATCAICLDYFTDPVMTDCGHNFCRECIRRCWGQPEGPYACPECRELSAQRNLRPNRP LAKMAEMARRLHPPSPVPQGVCAAHREPLTTFCGDDLSLLCPICERSEHWTHRVRPLQEAADDLKGRLEK SLEHLRKQMEDAMLFQAQAEETCALWQKMVESQRQNVLGEFERLRRLLAEEEQQLLQKLEEEELEVLPRL REGAARLGQQSTQLAALISELESRCQLPALGLLQLCIECCALEREASIAKDIKDALCRVQDVKLQPPAVV PMELRTVCRVPGLVETLRRFRGDITLDPDTANPELVLSEDRRSVQRGEQRQALPDNPERFDPGPCVLGQE RITSGRHYWEVEVGDQTSWALGVCKETANRKEKGELSAGNGFWILVFLGSFYNSNEPAFSPLRDPPKRVG IFLDYEAGHLSFYSATDGSLLFIFPETLFSGTLRPLFSPLSSSPTPMTICRLIGVSGDTLGPQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC020102 |
| ORF Size | 1449 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020102.1 |
| RefSeq Size | 2322 bp |
| RefSeq ORF | 1451 bp |
| Locus ID | 94091 |
| Gene Summary | E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218513 | Trim11 (untagged) - Mouse tripartite motif protein 11 (cDNA clone MGC:28318 IMAGE:4014766), (10ug) |
CNY 6,940.00 |
|
| MR207741 | Trim11 (Myc-DDK-tagged) - Mouse tripartite motif protein 11 (cDNA clone MGC:28318 IMAGE:4014766) |
CNY 3,656.00 |
|
| MR207741L3 | Lenti ORF clone of Trim11 (Myc-DDK-tagged) - Mouse tripartite motif protein 11 (cDNA clone MGC:28318 IMAGE:4014766) |
CNY 4,660.00 |
|
| MR207741L4 | Lenti ORF clone of Trim11 (mGFP-tagged) - Mouse tripartite motif protein 11 (cDNA clone MGC:28318 IMAGE:4014766) |
CNY 4,660.00 |
