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Ppm1b (BC018556) Mouse Tagged ORF Clone
CAT＃: MG207647
- TrueORF®
Ppm1b (tGFP-tagged) - Mouse protein phosphatase 1B, magnesium dependent, beta isoform (cDNA clone MGC:18462 IMAGE:3987825)
ORF Plasmid: DDK
"BC018556" in other vectors (4)
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CNY 5,230.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PP2CB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207647 representing BC018556
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCATTTTTGGATAAACCCAAAACTGAAAAGCACAATGCTCACGGTGCTGGGAATGGTCTGCGTT ATGGCCTGAGCAGTATGCAAGGATGGAGAGTAGAAATGGAAGATGCACACACAGCTGTTGTGGGTATTCC TCACGGCTTGGACAACTGGTCGTTTTTTGCAGTTTATGATGGTCATGCTGGATCCCGAGTGGCAAATTAC TGTTCAACACATCTATTAGAACACATCACCACCAATGAAGACTTCAGGGCAGCTGACAAATCAGGCTCTG CTCTCGAGCCTTCAGTAGAAAGTGTGAAGACTGGTATCAGGACTGGCTTTTTGAAAATTGATGAATATAT GCGTAACTTCTCAGACCTGAGGAACGGGATGGACAGGAGCGGCTCGACTGCAGTGGGCGTGATGGTCTCC CCTACACACATGTACTTCATCAACTGTGGTGACTCTCGAGCTGTTCTGTGTAGGAACGGACAGGTCTGCT TTTCTACCCAGGATCACAAACCTTGTAATCCAGTGGAGAAGGAGCGCATCCAAAACGCAGGAGGCAGTGT GATGATCCAGCGTGTGAACGGCTCACTAGCAGTGTCTCGGGCTCTGGGGGACTATGACTACAAGTGTGTG GATGGCAAGGGCCCTACAGAGCAGCTTGTTTCTCCAGAGCCTGAGGTTTATGAGATTGTGAGAGCAGAAG AGGATGAGTTTGTCGTCTTGGCCTGTGATGGGATCTGGGATGTGATGAGCAATGAGGAGCTCTGTGAGTT TGTTAAGTCTAGGCTTGAGGTGTCGGACGACCTGGAGAATGTGTGCAATTGGGTAGTGGACACTTGTTTA CATAAGGGAAGTCGAGATAACATGAGTGTTGTATTAGTTTGCTTTTCAAATGCCCCCAAGGTCTCAGAGG AAGCCGTGAAGAGAGATTCAGAGTTGGATAAGCACTTGGAATCACGGGTTGAAGAAATCATGCAGAAGTC TGGGGAGGAAGGAATGCCTGATCTTGCCCATGTGATGCGCATTTTGTCTGCAGAAAATATCCCGAATTTA CCTCCCGGTGGAGGTCTCGCTGGCAAGCGCCATGTTATTGAAGCTGTTTATAGTAGACTTAATCCACACA AAGACAATGATGGGGCCTCCGATGAAGCAGAGGAAGGTGGATCACAGGGGAAATTGGTGGAAGCTCTCAG GCAAATGAGAGTTAACCATAGGGGAAACTACCGACAGCTGCTGGAGGAGATGCTGACTAGTTACAGGCTA GCTAAAGTAGAGGGAGAAGAGAGCCCGGCTGACCCAGCGGCTGCAGCTGCTTCTTCGAACAGTGATGGTG GAAACCCAGTGGCAATGCAGGAGAGAGACACTGAGGGTGGTCCTGCTGGCCTAGACAGCCGTAATGAAGA TGCAGGGACAAAGAGGAGTGCTGAAAATATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207647 representing BC018556
Red=Cloning site Green=Tags(s) MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLDNWSFFAVYDGHAGSRVANY CSTHLLEHITTNEDFRAADKSGSALEPSVESVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMVS PTHMYFINCGDSRAVLCRNGQVCFSTQDHKPCNPVEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCV DGKGPTEQLVSPEPEVYEIVRAEEDEFVVLACDGIWDVMSNEELCEFVKSRLEVSDDLENVCNWVVDTCL HKGSRDNMSVVLVCFSNAPKVSEEAVKRDSELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNL PPGGGLAGKRHVIEAVYSRLNPHKDNDGASDEAEEGGSQGKLVEALRQMRVNHRGNYRQLLEEMLTSYRL AKVEGEESPADPAAAAASSNSDGGNPVAMQERDTEGGPAGLDSRNEDAGTKRSAENI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC018556 |
| ORF Size | 1431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018556.1 |
| RefSeq Size | 2365 bp |
| RefSeq ORF | 1433 bp |
| Locus ID | 19043 |
| Gene Summary | Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218224 | Ppm1b (untagged) - Mouse protein phosphatase 1B, magnesium dependent, beta isoform (cDNA clone MGC:18462 IMAGE:3987825), (10ug) |
CNY 6,940.00 |
|
| MR207647 | Ppm1b (Myc-DDK-tagged) - Mouse protein phosphatase 1B, magnesium dependent, beta isoform (cDNA clone MGC:18462 IMAGE:3987825) |
CNY 4,750.00 |
|
| MR207647L3 | Lenti ORF clone of Ppm1b (Myc-DDK-tagged) - Mouse protein phosphatase 1B, magnesium dependent, beta isoform (cDNA clone MGC:18462 IMAGE:3987825) |
CNY 6,650.00 |
|
| MR207647L4 | Lenti ORF clone of Ppm1b (mGFP-tagged) - Mouse protein phosphatase 1B, magnesium dependent, beta isoform (cDNA clone MGC:18462 IMAGE:3987825) |
CNY 6,650.00 |
