Cndp2 (BC005532) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	0610010E05Rik; C76600; Cn2; Dip-2; Pep-1; Pep1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207616 representing BC005532 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCCCTCAAAGCTGTCTTCCAGTATATCGATGAAAACCAGGACCGCTACGTCAAGAAACTTGCAG AATGGGTGGCCATCCAGAGCGTGTCCGCATGGCCGGAGAAGAGAGGAGAGATCAGAAGGATGATGGAGGT GGCAGCTGCCGATGTCCAGAGGCTGGGGGGCTCCGTGGAACTGGTGGATATTGGGAAGCAGAAGCTCCCA GATGGCTCGGAGATACCACTTCCTCCCATTCTGCTGGGCAAGCTAGGCAGCGACCCCCAGAAGAAAACCG TGTGCATTTACGGGCACCTGGACGTGCAGCCTGCGGCCCTGGAGGACGGGTGGGACAGCGAGCCCTTCAC CTTGGTGGAGCGGGAAGGCAAGCTGTATGGGAGAGGCTCCACGGACGATAAGGGGCCAGTGGCCGGGTGG ATGAATGCCCTGGAAGCCTATCAGAAGACTGGTCAGGAGATCCCAGTCAACCTGCGGTTCTGCCTGGAAG GCATGGAGGAGTCTGGCTCCGAAGGCCTGGATGAGCTCATTTTTGCCCAGAAAGACAAGTTCTTCAAAGA TGTGGACTATGTGTGCATTTCAGACAATTACTGGCTGGGGAAGAATAAGCCGTGTATCACGTACGGCCTC AGAGGCATCTGTTACTTCTTCATCGAGGTGGAATGCAGTGACAAAGACCTCCATTCTGGGGTATATGGAG GCTCAGTGCACGAGGCCATGACGGATCTCATTTCACTGATGGGCTGCCTGGTAGACAAGAAGGGGAAGAT CCTCATCCCGGGCATCAACGATGCCGTGGCGCCTGTGACGGATGAGGAGCATGCGCTCTACGACCACATT GACTTCGATATGGAAGAGTTCGCCAAGGACGTGGGGGCTGAGACCCTTCTGCACAGCTGCAAGAAAGACA TCCTGATGCACAGATGGCGATACCCGTCCCTCTCCCTGCATGGGATCGAAGGGGCCTTCTCCGGTTCAGG GGCCAAGACTGTGATCCCCAGAAAGGTGGTCGGCAAGTTCTCCATCAGGCTCGTGCCAGACATGATACCG GAAGTTGTTAGCGAGCAAGTTTCAAGCTACTTGTCAAAAAAATTTGCTGAACTGCAGAGCCCCAACAAGT TCAAGGTGTACATGGGCCACGGCGGGAAGCCCTGGGTGTCTGACTTCAACCATCCTCATTACCAGGCGGG GAGAAGAGCCCTGAAGACAGTGTTTGGTGTTGAGCCTGACTTGACCAGAGAAGGTGGCAGTATTCCTGTG ACCTTGACCTTTCAAGAGGCCACAGGCAAAAATGTCATGCTATTGCCAGTGGGGTCAGCAGATGACGGTG CCCACTCGCAGAATGAGAAGCTCAACAGGCTCAACTACATAGAAGGAACCAAAATGCTGGCTGCATATCT GTATGAGGTGTCTCAGCTGAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207616 representing BC005532 Red=Cloning site Green=Tags(s) MSALKAVFQYIDENQDRYVKKLAEWVAIQSVSAWPEKRGEIRRMMEVAAADVQRLGGSVELVDIGKQKLP DGSEIPLPPILLGKLGSDPQKKTVCIYGHLDVQPAALEDGWDSEPFTLVEREGKLYGRGSTDDKGPVAGW MNALEAYQKTGQEIPVNLRFCLEGMEESGSEGLDELIFAQKDKFFKDVDYVCISDNYWLGKNKPCITYGL RGICYFFIEVECSDKDLHSGVYGGSVHEAMTDLISLMGCLVDKKGKILIPGINDAVAPVTDEEHALYDHI DFDMEEFAKDVGAETLLHSCKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPDMIP EVVSEQVSSYLSKKFAELQSPNKFKVYMGHGGKPWVSDFNHPHYQAGRRALKTVFGVEPDLTREGGSIPV TLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRLNYIEGTKMLAAYLYEVSQLKN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC005532
ORF Size	1427 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005532, AAH05532
RefSeq Size	2152 bp
RefSeq ORF	1427 bp
Locus ID	66054
Gene Summary	Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis.[UniProtKB/Swiss-Prot Function]

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