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Unc84b (BC098208) Mouse Tagged ORF Clone
CAT＃: MG207572
- TrueORF®
Unc84b (tGFP-tagged) - Mouse unc-84 homolog B (C. elegans) (cDNA clone MGC:106463 IMAGE:6827666)
ORF Plasmid: DDK
"BC098208" in other vectors (4)
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CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SUN2, C030011B15 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207572 representing BC098208
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAGACGAAGCCAGCGCCTCACTCGCTACTCTCAGGATGATAACGATGGCGGCAGCAGCAGCAGTG GTGCGAGCTCCGTGGCAGGAAGCCAGGGCACCGTGTTTAAAGACAGTCCTCTCAGGACTTTGAAGAGGAA ATCCAGCAACATGAAGCACCTGTCCCCAGCTCCACAGCTGGGCCCCTCCTCTGACTCCCACACCTCCTAC TACAGCGAGTCTGTGGTTCGAGAGTCCTACATCGGCAGCCCCCGGGCTGTGTCCCTCGCCAGGAGTGCCC TCCTGGATGACCACCTACACAGTGAGCCCTACTGGAGCGGGGACCTTCGGGGGAGGAGGAGGAGAGGAAC AGGTGGTTCTGAGAGCAGCAAGGCCAATGGGCTCACCGCGGAGAGCAAGGCCTCAGAAGACTTTTTCGGA TCTTCCTCAGGCTATTCTTCAGAGGATGACCTTGCAGGCTACACGGACTCAGACCAGCACAGCTCGGGGT CCAGGTTAAGGAGTGCAGCATCTCGGGCCGGCTCCTTTGTCTGGACTCTGGTCACTTTTCCAGGCCGCCT CTTTGGTCTTCTCTACTGGTGGATTGGCACCACCTGGTACCGCCTGACAACTGCTGCCTCCCTCCTGGAT GTCTTCGTCCTAACCAGGCACTTCTCGCTGAACCTGAAGAGTTTTCTGTGGTTCCTTCTGCTCTTGCTAC TCCTGACTGGTCTGACCTACGGTGCTTGGCATTTTTACCCCTTAGGGCTGCAGACATTGCAACCCGCTGT GGTCTCCTGGTGGGCAGCAAAAGAGAGCAGGAAGCAGCCAGAGGTGTGGGAATCCAGAGACGCCTCCCAG CACTTCCAGGCTGAGCAGCGCGTTCTCTCCCGGGTTCACTCTCTGGAGCGGCGTCTGGAAGCCCTTGCTG CAGACTTTTCCTCCAACTGGCAGAAGGAGGCCATACGGCTGGAACGCCTGGAGCTGCGGCAGGGGGCTGC TGGCCATGGAGGAGGCAGTAGCCTGAGCCATGAAGATGCCCTGTCTCTCCTAGAAGGGTTGGTGAGCCGC CGCGAGGCTACCCTGAAGGAGGACTTGCGCAGGGACACAGTGGCTCATATCCAGGAAGAATTGGCTACCC TGAGGGCAGAGCATCACCAAGACTCGGAAGATCTCTTCAAGAAGATCGTCCAGGCCTCTCAGGAGTCCGA AGCCCGAGTCCAGCAGCTGAAGACAGAATGGAAAAGCATGACCCAGGAGGCCTTCCAGGAGAGCTCTGTG AAGGAGCTGGGACGGCTGGAAGCCCAGCTGGCCAGCCTGCGGCAGGAGCTGGCTGCCCTGACTCTGAAGC AGAACTCGGTGGCAGATGAAGTGGGCCTGCTGCCACAGAAGATCCAGGCTGCCAGGGCTGATGTGAGCGG GAAGTACCCAGAGCCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207572 representing BC098208
Red=Cloning site Green=Tags(s) MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSY YSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFG SSSGYSSEDDLAGYTDSDQHSSGSRLRSAASRAGSFVWTLVTFPGRLFGLLYWWIGTTWYRLTTAASLLD VFVLTRHFSLNLKSFLWFLLLLLLLTGLTYGAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDASQ HFQAEQRVLSRVHSLERRLEALAADFSSNWQKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLVSR REATLKEDLRRDTVAHIQEELATLRAEHHQDSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQESSV KELGRLEAQLASLRQELAALTLKQNSVADEVGLLPQKIQAARADVSGKYPEPY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC098208 |
| ORF Size | 1421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC098208, AAH98208 |
| RefSeq Size | 4142 bp |
| RefSeq ORF | 1421 bp |
| Locus ID | 223697 |
| Gene Summary | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
,null,
Scientific Reports
,PubMed ID 33686165
[Unc84b]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217993 | Unc84b (untagged) - Mouse unc-84 homolog B (C. elegans) (cDNA clone MGC:106463 IMAGE:6827666), (10ug) |
CNY 6,940.00 |
|
| MR207572 | Unc84b (Myc-DDK-tagged) - Mouse unc-84 homolog B (C. elegans) (cDNA clone MGC:106463 IMAGE:6827666) |
CNY 3,656.00 |
|
| MR207572L3 | Lenti ORF clone of Unc84b (Myc-DDK-tagged) - Mouse unc-84 homolog B (C. elegans) (cDNA clone MGC:106463 IMAGE:6827666) |
CNY 4,560.00 |
|
| MR207572L4 | Lenti ORF clone of Unc84b (mGFP-tagged) - Mouse unc-84 homolog B (C. elegans) (cDNA clone MGC:106463 IMAGE:6827666) |
CNY 4,560.00 |
