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Inpp5k (NM_008916) Mouse Tagged ORF Clone
CAT＃: MG207493
- TrueORF®
Inpp5k (tGFP-tagged) - Mouse putative phosphatase (Pps)
ORF Plasmid: DDK
"NM_008916" in other vectors (4)
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CNY 4,470.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C62; Pps; SKIP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207493 representing NM_008916
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCACGGAGACAGGAACACACCGGGTTACCGCGAGGGAATAATGTCGGCTGTGAGTCTAAGGAGGC CGAGCGCACCGAAAGGCTTTGCACTCAGCGTACATGTTGTGACGTGGAATGTGGCCTCCGCAGCCCCCAC TGTAGACCTCAGTGACCTACTTCAACTGAACAACCAAGACCTGAATCTGGACATATATATCATTGGTTTG CAGGAAATGAATTTTGGAATCATAAGCCTTCTTTCTGATGCTGCTTTTGAAGACCCATGGAGCAGTCTCT TCATGGATATGCTTTCCCCACTGAACTTTGTCAAGATCTCTCAAGTCCGTATGCAGGGGCTTCTCTTACT GGTCTTTGCCAAGTACCAGCATTTGCCCTACATCCAGATCATCTCTACGAAATCCACCCCCACTGGCCTC TACGGGTACTGGGGGAACAAAGGGGGAGTCAACGTCTGCCTGAAGCTTTATGGCTACTACGTCAGCATCA TCAATTGCCACCTGCCTCCTCATATGTATAACAATGACCAGCGCCTGGAACACTTTGACCGAATCTTGGA GAGTCTGACTTTTGAGGGATATGATGTCCCCAACATCCTGGACCATGACCTCATTCTGTGGTTTGGAGAC ATGAACTTTCGAATTGAGGACTTTGGGTTGCTCTTTGTGCAGGAGAGTATAACGAGGAAGTACTACAAAG AGCTGTGGGAGAAAGATCAGCTCTTCATTGCCAAGAAAAATGACCAGCTGCTCCGCGAGTTCCAGGAAGG CCCGCTGCTCTTCCCACCCACCTACAAGTTTGACCGTCACTCCAACAACTATGACACCAGTGAGAAAAAA CGCAAGCCTGCGTGGACTGACCGCATCCTGTGGAGGTTGAAGCGGCAGCCGTCCCAGGCCAGCCCCTTGG CCTCCAGTGTACCAACCTCCTACTTCTTGCTGACACTGAAGAACTATGTCAGCCACATGGCATACAGCAT CAGTGACCATAAGCCTGTCACTGGCACCTTTGACTTGGAGCTGAACCCACTGATGTCTGTCCCACTGATC ACTATGATGCCTGAACACCTGTGGACCATGGAGAACGACATGCTGATCAGCTACACCTCCACCCCAGAGT TCCTCAGCAGCTCCTGGGACTGGATCGGACTATACAAGGTGGGGATGCGTCACATTAATGACTATGTAGC CTATGTCTGGGTCGGCGACAACCAAGTCTCCTATGGTAACAACCCAAACCAGGTGTATATCAACATCAGT GCTATCCCTGACACTGAGGATCAGTTTCTTCTCTGTTACTATAGCAACAACCTGCACTCTGTAGTGGGGA TAAGTCAACCCTTCAAGATCCCGATTCGCTCCTTTTTGAGAGAGGACACACTGTATGAGCCTGAGCCACA GATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207493 representing NM_008916
Red=Cloning site Green=Tags(s) MQHGDRNTPGYREGIMSAVSLRRPSAPKGFALSVHVVTWNVASAAPTVDLSDLLQLNNQDLNLDIYIIGL QEMNFGIISLLSDAAFEDPWSSLFMDMLSPLNFVKISQVRMQGLLLLVFAKYQHLPYIQIISTKSTPTGL YGYWGNKGGVNVCLKLYGYYVSIINCHLPPHMYNNDQRLEHFDRILESLTFEGYDVPNILDHDLILWFGD MNFRIEDFGLLFVQESITRKYYKELWEKDQLFIAKKNDQLLREFQEGPLLFPPTYKFDRHSNNYDTSEKK RKPAWTDRILWRLKRQPSQASPLASSVPTSYFLLTLKNYVSHMAYSISDHKPVTGTFDLELNPLMSVPLI TMMPEHLWTMENDMLISYTSTPEFLSSSWDWIGLYKVGMRHINDYVAYVWVGDNQVSYGNNPNQVYINIS AIPDTEDQFLLCYYSNNLHSVVGISQPFKIPIRSFLREDTLYEPEPQI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008916 |
| ORF Size | 1404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008916.2, NP_032942.1 |
| RefSeq Size | 2660 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 19062 |
| UniProt ID | Q8C5L6 |
| Gene Summary | Inositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5-trisphosphate (By similarity). Negatively regulates assembly of the actin cytoskeleton. Controls insulin-dependent glucose uptake among inositol 3,4,5-trisphosphate phosphatases; therefore, is the specific regulator for insulin signaling in skeletal muscle (PubMed:22247557, PubMed:22751929).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203424 | Inpp5k (untagged) - Mouse inositol polyphosphate 5-phosphatase K (Inpp5k), (10ug) |
CNY 3,656.00 |
|
| MR207493 | Inpp5k (Myc-DDK-tagged) - Mouse inositol polyphosphate 5-phosphatase K (Inpp5k) |
CNY 3,656.00 |
|
| MR207493L3 | Lenti ORF clone of Inpp5k (Myc-DDK-tagged) - Mouse inositol polyphosphate 5-phosphatase K (Inpp5k) |
CNY 5,990.00 |
|
| MR207493L4 | Lenti ORF clone of Inpp5k (mGFP-tagged) - Mouse inositol polyphosphate 5-phosphatase K (Inpp5k) |
CNY 5,990.00 |
