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Dmap1 (NM_023178) Mouse Tagged ORF Clone
CAT＃: MG207480
- TrueORF®
Dmap1 (tGFP-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1)
ORF Plasmid: DDK
"NM_023178" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1500016M21Rik; Dmtap1; DNMAP1; DNMTAP1; mKIAA1425 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207480 representing NM_023178
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACGGGCGCAGATGTACGAGACATTCTAGAACTCGGGGGTCCAGAGGGAGATGCCGCCTCTGGGA CCATCAGCAAAAAGGATATTATCAACCCGGACAAGAAAAAGTCCAAGAAGTCCTCAGAGACGCTGACCTT CAAGAGGCCTGAGGGCATGCATCGGGAGGTCTATGCTTTGCTTTACTCTGACAAAAAGGATGCACCCCCA CTGCTGCCCAGTGACACTGGTCAGGGGTATCGGACAGTGAAGGCGAAACTGGGGTCCAAGAAGGTTCGCC CTTGGAAATGGATGCCTTTTACTAACCCAGCTCGAAAGGACGGCGCTATGTTTTTCCACTGGCGACGAGC GGCGGAGGAGGGCAAGGACTACCCTTTTGCCAGGTTCAATAAGACGGTGCAGGTGCCCGTGTACTCAGAG CAGGAGTACCAACTCTACCTTCATGATGACGCATGGACTAAGGCAGAGACTGACCACCTATTTGACCTCA GCCGCCGATTTGATCTGCGCTTCGTAGTTATTCACGATCGGTATGACCACCAGCAGTTCAAGAAGCGTTC TGTGGAGGACCTGAAAGAGAGGTACTACCACATTTGTGCCAAGCTTGCCAACGTGAGGGCTGTGCCAGGC ACAGATCTCAAGATACCAGTGTTTGATGCTGGGCATGAGAGACGGCGGAAGGAACAGCTAGAGCGGCTTT ACAACCGAACCCCAGAGCAGGTGGCAGAGGAGGAGTACCTCCTACAGGAGCTGCGTAAGATTGAGGCCCG GAAAAAAGAGCGGGAGAAGCGCAGCCAAGACCTGCAGAAGCTGATTACAGCAGCAGACACCACTGCAGAG CAGCGGCGCACGGAACGCAAGGCTCCCAAGAAGAAGCTACCCCAAAAGAAGGAGGCTGAGAAGCCGGCTG TCCCTGAGACTGCAGGCATCAAGTTTCCAGATTTTAAGTCGGCAGGTGTCACGCTACGGAGCCAGCGGAT GAAGCTACCCAGCTCTGTGGGTCAGAAGAAGATCAAGGCGCTGGAACAGATGCTGCTGGAACTTGGTGTG GAGCTGAGCCCTACCCCCACAGAGGAGCTGGTGCATATGTTCAATGAGTTGCGGAGCGACCTGGTGTTAC TCTACGAGCTCAAGCAGGCCTGTGCCAACTGTGAATATGAGCTACAGATGCTGCGGCACCGGCACGAGGC CCTGGCTCGGGCAGGAGTGCTGGGGGCCCCTGCCGCAGCAGCAGTGGGACCAACCCCGGCTTCTGCTGAG CCAACAGTGTCTGAATCTGGACTTGGTCTGGACCCCACCAAGGATACCATCATTGATGTCGTGGGTGCAC CCCTCACACCCAATTCGCGGAAACGACGGGAATCAGCCTCCAGCTCATCTTCTGTGAAGAAAGCCAAGAA ACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207480 representing NM_023178
Red=Cloning site Green=Tags(s) MATGADVRDILELGGPEGDAASGTISKKDIINPDKKKSKKSSETLTFKRPEGMHREVYALLYSDKKDAPP LLPSDTGQGYRTVKAKLGSKKVRPWKWMPFTNPARKDGAMFFHWRRAAEEGKDYPFARFNKTVQVPVYSE QEYQLYLHDDAWTKAETDHLFDLSRRFDLRFVVIHDRYDHQQFKKRSVEDLKERYYHICAKLANVRAVPG TDLKIPVFDAGHERRRKEQLERLYNRTPEQVAEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAE QRRTERKAPKKKLPQKKEAEKPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKIKALEQMLLELGV ELSPTPTEELVHMFNELRSDLVLLYELKQACANCEYELQMLRHRHEALARAGVLGAPAAAAVGPTPASAE PTVSESGLGLDPTKDTIIDVVGAPLTPNSRKRRESASSSSSVKKAKKP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023178 |
| ORF Size | 1404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023178.2, NP_075667.1 |
| RefSeq Size | 1543 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 66233 |
| UniProt ID | Q9JI44 |
| Gene Summary | Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216224 | Dmap1 (untagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1), (10ug) |
CNY 6,080.00 |
|
| MR207480 | Dmap1 (Myc-DDK-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1) |
CNY 3,656.00 |
|
| MR207480L3 | Lenti ORF clone of Dmap1 (Myc-DDK-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1) |
CNY 5,230.00 |
|
| MR207480L4 | Lenti ORF clone of Dmap1 (mGFP-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1) |
CNY 5,230.00 |
