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Nup50 (NM_016714) Mouse Tagged ORF Clone
CAT＃: MG207453
- TrueORF®
Nup50 (tGFP-tagged) - Mouse nucleoporin 50 (Nup50)
ORF Plasmid: DDK
"NM_016714" in other vectors (3)
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CNY 4,470.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700030K07Rik; AI413123; Npap60 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207453 representing NM_016714
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGAGAGTTGCGGAGAAGGAGTTGACTGACAGGAACTGGGATGAGGAAGACGAAGTTGAAGAGA TGGGAACATTCTCAGTGGCCAGTGAGGAAGTCATGAAGAACAGAGCCGTAAAGAAGGCAAAGCGCAGAAA CGTTGGATTTGAATCTGATAGCGGAGGAGCCTTTAAAGGTTTCAAAGGTTTGGTTGTGCCTTCTGGAGGA GGAGGGTTTTCTGGATTTGGTGGCTCTGGAGGAAAGCCTCTGGAAGGACTGACAAATGGAAACAGCACAG ACAATGCCACGCCCTTCTCCAATGTAAAGACAGCAGCAGAGCCCAAGGCAGCCTTTGGTTCTTTTGCTGT GAATGGCCCTACTACCTTGGTGGATAAAAAGATTTCAAGTCCCAAATGCAATAACAGCAATCAGCCGCCC TCCTCCGGCCCTGCTTCCAGTACCGCCTGCCCTGGGAATGCCTATCACAAGCAGCTGGCTGGCTTGAACT GCTCCGTCCGCGATTGGATAGTGAAGCACGTGAACACAAACCCGCTTTGTGACCTGACTCCCATTTTTAA AGACTATGAGAGATACTTGGCGACGATCGAGAAGCAGCTTGAGAATGGAGGCGGCAGCAGTTCTGAGAGC CAGACAGACAGGGCGACGGCTGGAATGGAGCCTCCTTCCCTTTTTGGTTCAACAAAACTACAGCAAGAGT CACCATTTTCATTTCATGGCAACAAAGCGGAGGACACATCTGAAAAGGTGGAGTTTACAGCAGAAAAGAA ATCGGACGCAGCACAAGGAGCAACAAGTGCCTCGTTTAGTTTCGGCAAGAAAATTGAGAGCTCGGCTTTG GGCTCGTTAAGCTCTGGCTCCCTAACTGGGTTTTCATTCTCTGCTGGAAGCTCCAGCTTGTTTGGTAAAG ATGCTGCCCAGAGTAAAGCAGCCTCTTCGCTGTTCTCTGCTAAAGCATCCGAGAGTCCGGCAGGAGGCGG CAGCAGCGAGTGCAGAGATGGTGAAGAAGAGGAGAATGACGAGCCACCCAAGGTAGTGGTGACCGAAGTA AAGGAAGAGGATGCTTTCTACTCCAAAAAATGTAAACTATTTTACAAGAAAGACAACGAATTTAAAGAGA AGGGTGTGGGGACCCTGCATTTAAAACCCACAGCAACTCAGAAGACCCAGCTCTTGGTGCGGGCAGACAC CAACCTAGGCAACATACTGCTGAATGTTCTGATCGCCCCCAACATGCCGTGCACCCGGACAGGAAAGAAC AACGTCCTTATCGTCTGTGTCCCCAACCCCCCACTCGATGAGAAGCAGCCCACTCTCCCGGCCACCATGC TGATCCGGGTGAAGACGAGCGAGGATGCCGATGAATTGCACAAGATTTTACTGGAGAAAAAGGATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207453 representing NM_016714
Red=Cloning site Green=Tags(s) MAKRVAEKELTDRNWDEEDEVEEMGTFSVASEEVMKNRAVKKAKRRNVGFESDSGGAFKGFKGLVVPSGG GGFSGFGGSGGKPLEGLTNGNSTDNATPFSNVKTAAEPKAAFGSFAVNGPTTLVDKKISSPKCNNSNQPP SSGPASSTACPGNAYHKQLAGLNCSVRDWIVKHVNTNPLCDLTPIFKDYERYLATIEKQLENGGGSSSES QTDRATAGMEPPSLFGSTKLQQESPFSFHGNKAEDTSEKVEFTAEKKSDAAQGATSASFSFGKKIESSAL GSLSSGSLTGFSFSAGSSSLFGKDAAQSKAASSLFSAKASESPAGGGSSECRDGEEEENDEPPKVVVTEV KEEDAFYSKKCKLFYKKDNEFKEKGVGTLHLKPTATQKTQLLVRADTNLGNILLNVLIAPNMPCTRTGKN NVLIVCVPNPPLDEKQPTLPATMLIRVKTSEDADELHKILLEKKDA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016714 |
| ORF Size | 1398 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016714.2, NP_057923.2 |
| RefSeq Size | 4766 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 18141 |
| UniProt ID | Q9JIH2 |
| Gene Summary | Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:10811608). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:16222336). Interacts with regulatory proteins of cell cycle progression including CDKN1B (PubMed:10891500, PubMed:10811608). This interaction is required for correct intracellular transport and degradation of CDKN1B (PubMed:10811608).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR207453 | Nup50 (Myc-DDK-tagged) - Mouse nucleoporin 50 (Nup50) |
CNY 3,656.00 |
|
| MR207453L3 | Lenti ORF clone of Nup50 (Myc-DDK-tagged) - Mouse nucleoporin 50 (Nup50) |
CNY 5,990.00 |
|
| MR207453L4 | Lenti ORF clone of Nup50 (mGFP-tagged) - Mouse nucleoporin 50 (Nup50) |
CNY 5,990.00 |
