Tcf7l2 (NM_009333) Mouse Tagged ORF Clone
CAT#: MG207325
- TrueORF®
Tcf7l2 (tGFP-tagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2)
ORF Plasmid: DDK
"NM_009333" in other vectors (4)
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CNY 4,470.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Tcf-4; Tcf4; TCF4B; TCF4E |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207325 representing NM_009333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCAGCTGAACGGCGGTGGAGGAGATGACCTAGGCGCTAACGACGAGCTGATCTCCTTCAAAGACG AAGGCGAGCAGGAGGAGAAGAACTCGGAAAACTCCTCGGCGGAAAGGGATTTAGCCGATGTCAAGTCCTC GCTGGTCAATGAATCAGAGACGAATCAAAACAGCTCCTCCGATTCCGAGGCGGAAAGACGGCCTCCGCCT CGCTCCGAAAGTTTCCGAGATAAATCCCGGGAAAGTTTGGAAGAAGCGGCCAAGAGGCAAGATGGAGGGC TCTTTAAGGGGCCACCGTATCCCGGCTACCCCTTCATCATGATCCCCGACCTGACGAGCCCCTACCTCCC CAACGGATCGCTCTCGCCCACCGCCCGAACCTATCTTCAGATGAAATGGCCACTGCTTGATGTCCAAGCA GGAAGCCTCCAGAGCAGACAAACCCTCAAGGATGCTCGTTCGCCGTCGCCAGCACACATCGTTTCGAACA AAGTACCGGTGGTGCAACACCCCCACCATGTCCACCCACTCACGCCTCTCATCACGTACAGCAATGAACA CTTCACCCCGGGAAATCCACCTCCGCACTTACCAGCTGACGTAGACCCCAAAACAGGAATCCCAAGGCCT CCGCACCCTCCAGATATCTCTCCATATTACCCGCTGTCGCCCGGCACCGTAGGACAAATCCCCCATCCGC TAGGATGGTTAGTACCACAGCAAGGTCAGCCTGTGTACCCAATCACGACAGGAGGATTCAGACACCCCTA CCCCACAGCGCTGACAGTCAACGCATCTATGTCTAGGTTCCCTCCCCATATGGTCCCTCCCCATCACACT CTGCACACGACCGGCATCCCTCACCCGGCCATCGTCACACCGACAGTCAAGCAGGAATCCTCCCAGAGTG ACGTCGGCTCACTCCACAGCTCAAAGCATCAGGACTCCAAAAAGGAAGAAGAGAAGAAGAAGCCCCACAT AAAGAAGCCCCTTAATGCATTCATGTTGTATATGAAAGAGATGAGAGCGAAGGTGGTGGCCGAATGCACA TTGAAAGAGAGTGCAGCCATCAACCAGATTCTCGGGCGCAGGTGGCACGCCCTGTCCAGGGAAGAACAGG CAAAATACTACGAGCTGGCCCGGAAGGAACGACAGCTTCACATGCAGCTGTACCCTGGCTGGTCTGCACG GGATAACTATGGGAAGAAGAAGAAGAGAAAAAGAGACAAGCAGCCGGGGGAAACCAACGAACACAGCGAA TGTTTCCTAAATCCTTGCCTTTCGCTTCCTCCGATCACAGGAGAAAAAAAAAGTGCGTTCGCTACATACA AGGTGAAGGCAGCTGCCTCAGCCCACCCTCTTCAGATGGAAGCTTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207325 representing NM_009333
Red=Cloning site Green=Tags(s) MPQLNGGGGDDLGANDELISFKDEGEQEEKNSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPP RSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQA GSLQSRQTLKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRP PHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHT LHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECT LKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSE CFLNPCLSLPPITGEKKSAFATYKVKAAASAHPLQMEAY TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009333 |
ORF Size | 1377 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009333.4 |
RefSeq Size | 3230 bp |
RefSeq ORF | 1380 bp |
Locus ID | 21416 |
Gene Summary | Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216094 | Tcf7l2 (untagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 3, (10ug) |
CNY 5,990.00 |
|
MR207325 | Tcf7l2 (Myc-DDK-tagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 3 |
CNY 3,656.00 |
|
MR207325L3 | Lenti ORF clone of Tcf7l2 (Myc-DDK-tagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 3 |
CNY 5,990.00 |
|
MR207325L4 | Lenti ORF clone of Tcf7l2 (mGFP-tagged) - Mouse transcription factor 7-like 2, T-cell specific, HMG-box (Tcf7l2), transcript variant 3 |
CNY 5,990.00 |