United States
Germany
Japan
United Kingdom
China
Blnk (NM_008528) Mouse Tagged ORF Clone
CAT＃: MG207297
- TrueORF®
Blnk (tGFP-tagged) - Mouse B-cell linker (Blnk)
ORF Plasmid: DDK
"NM_008528" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BASH; Bca; Ly-57; Ly57; Lyw-57; SLP-65 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207297 representing NM_008528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGCTGAATAAGATAACTGTCCCTGCCAGCCAGAAGCTGAGACAGCTTCAAAAGATGGTCCATG ATATTAAGAACAATGAAGGTGGAATAATGGACAAGATAAAAAAGCTAAAAGTCAAAGGCCCTCCAAGTGT TCCTCGAAGGGACTATGCATTAGACAGCCCTGCAGATGAAGAGGAGCAGTGGTCAGATGACTTCGACAGT GACTATGAAAATCCAGATGAACATTCGGACTCCGAGATGTATGTGATGCCTGCCGAGGAGACGGGCGACG ATTCCTATGAACCGCCTCCCGCTGAGCAGCAGACACGGGTGGTCCATCCAGCCCTGCCCTTCACGAGGGG CGAGTATGTAGATAATCGATCCAGCCAGCGGCACTCTCCGCCCTTCAGCAAGACACTTCCCAGTAAGCCC AGCTGGCCTTCAGCGAAAGCGAGGCTGGCCTCCACTCTGCCAGCCCCCAACTCTCTACAGAAGCCTCAAG TCCCCCCCAAGCCCAAAGACCTCCTTGAGGATGAGGCTGATTATGTGGTCCCTGTGGAAGATAACGATGA AAACTATATCCATCCCAGAGAAAGCAGCCCGCCGCCTGCTGAGAAGGCTCCCATGGTGAATAGATCAACC AAGCCAAACAGTTCCTCAAAGCACATGTCGCCTCCAGGGACTGTCGCAGGTCGAAACAGTGGGGTCTGGG ACTCCAAGTCATCTTTGCCTGCCGCACCATCCCCACTACCACGGGCTGGGAAGAAGCCAGCTACACCACT TAAGACTACTCCCGTTCCTCCCCTACCGAATGCATCAAATGTTTGTGAAGAAAAGCCTGTTCCTGCTGAG CGCCACCGAGGGTCTAGTCACAGACAAGACACTGTACAGTCACCAGTGTTTCCTCCCACCCAGAAACCTG TCCATCAAAAGCCTGTACCCTTGCCAAGGTTCCCAGAAGCGGGGAGCCCAGCTGCAGATGGACCGTTCCA CAGCTTCCCATTTAATTCGACGTTTGCAGACCAGGAGGCTGAACTGCTCGGTAAGCCCTGGTATGCTGGC GCCTGTGACCGCAAGTCTGCTGAAGAGGCCTTGCACAGATCCAACAAGGATGGATCGTTTCTTATTCGGA AGAGCTCTGGCCATGATTCCAAGCAGCCGTACACCCTAGTTGCGTTCTTTAACAAGCGAGTGTATAATAT TCCTGTACGGTTTATTGAAGCAACCAAACAGTATGCTTTGGGAAAGAAGAAAAATGGTGAAGAGTACTTC GGAAGTGTTGTGGAAATCGTCAACAGTCACCAGCACAACCCCCTGGTTCTTATTGACAGTCAGAATAACA CGAAAGATTCCACGAGACTGAAATATGCTGTGAAGGTTTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207297 representing NM_008528
Red=Cloning site Green=Tags(s) MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDS DYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKP SWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRST KPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAE RHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPRFPEAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAG ACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYF GSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008528 |
| ORF Size | 1371 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008528.4, NP_032554.2 |
| RefSeq Size | 2097 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 17060 |
| UniProt ID | Q9QUN3 |
| Gene Summary | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204614 | Blnk (untagged) - Mouse B-cell linker (Blnk), (10ug) |
CNY 2,000.00 |
|
| MR207297 | Blnk (Myc-DDK-tagged) - Mouse B-cell linker (Blnk) |
CNY 3,330.00 |
|
| MR207297L3 | Lenti ORF clone of Blnk (Myc-DDK-tagged) - Mouse B-cell linker (Blnk) |
CNY 5,230.00 |
|
| MR207297L4 | Lenti ORF clone of Blnk (mGFP-tagged) - Mouse B-cell linker (Blnk) |
CNY 5,230.00 |
