Dnm2 (BC010233) Mouse Tagged ORF Clone

CAT#: MG207259

  • TrueORF®

Dnm2 (tGFP-tagged) - Mouse dynamin 2 (cDNA clone MGC:7779 IMAGE:3499181)

ORF Plasmid: DDK tGFP



  "BC010233" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms b2b2159Clo; Dyn2; Udnm
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207259 representing BC010233
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTTGAAGCCATCGTGAAAAAACAGCTTGTAAAACTCAAAGAGCCGAGTTTGAAGTGTGTTGACC
TGGTGGTGTCGGAGCTAGCCACGGTCATTAAAAAGTGTGCTGAGAAGCTGAGTTCCTACCCCCGGCTGCG
AGAGGAGACCGAGCGAATTGTCACCACCTACATCAGGGAGCGAGAAGGGAGAACCAAGGACCAGATTCTT
CTTCTGATTGACATTGAGCAGTCGTACATCAACACAAACCATGAAGACTTCATTGGATTTGCCAATGCCC
AGCAGAGGAGCACGCAGCTGAACAAGAAGAGGGCCATACCCAATCAGGGGGAGATCTTGGTGATCCGCAG
GGGCTGGTTGACCATCAACAATATCAGCTTGATGAAGGGTGGCTCCAAGGAGTACTGGTTCGTGCTGACA
GCTGAGTCATTGTCTTGGTACAAGGATGAGGAGGAGAAAGAAAAGAAGTACATGCTGCCACTGGACAACC
TCAAGATCCGGGACGTGGAAAAGGGCTTCATGTCCAACAAGCACGTGTTTGCCATCTTCAACACAGAGCA
GAGGAATGTCTACAAGGACCTTCGACAGATTGAACTGGCTTGTGACTCCCAGGAAGATGTGGACAGCTGG
AAGGCTTCGTTCCTGCGCGCTGGGGTCTACCCAGAGAAGGACCAGGCAGAGAATGAGGATGGAGCACAAG
AGAACACCTTCTCCATGGACCCGCAGCTGGAGCGACAGGTGGAGACCATCCGTAACCTGGTGGACTCCTA
CGTGGCCATCATCAACAAGTCCATCCGTGACCTTATGCCAAAGACCATCATGCACCTCATGATCAACAAC
ACAAAGGCCTTCATCCACCATGAGCTGCTGGCTTACCTGTATTCATCAGCAGACCAGAGCAGCCTCATGG
AAGAGTCAGCCGAGCAGGCTCAGCGGCGGGACGACATGCTGCGCATGTACCACGCACTGAAGGAGGCCCT
CAACATTATTGGGGACATCAGCACCAGCACCGTGTCCACACCCGTGCCCCCACCGGTCGATGACACGTGG
CTCCAAAACACCAGCGGCCACAGCCCCACTCCACAGCGCCGACCTGTGTCCAGTGTGCACCCACCAGGCC
GGCCACCTGCAGTGCGGGGTCCTACACCGGGGCCTCCCCTGATTCCTATGCCTGTGGGGGCCACATCCTC
CTTCTCAGCACCTCCCATCCCATCCCGGCCTGGACCACAGAGTGTGTTTGCCAACAACGACCCCTTCTCT
GCGCCACCTCAGATACCATCTCGGCCAGCACGGATTCCCCCTGGTATCCCTCCAGGAGTGCCCAGGCGAC
CACCTCCATTGGCTCCTGCCCGACCTTTCTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207259 representing BC010233
Red=Cloning site Green=Tags(s)

MAFEAIVKKQLVKLKEPSLKCVDLVVSELATVIKKCAEKLSSYPRLREETERIVTTYIREREGRTKDQIL
LLIDIEQSYINTNHEDFIGFANAQQRSTQLNKKRAIPNQGEILVIRRGWLTINNISLMKGGSKEYWFVLT
AESLSWYKDEEEKEKKYMLPLDNLKIRDVEKGFMSNKHVFAIFNTEQRNVYKDLRQIELACDSQEDVDSW
KASFLRAGVYPEKDQAENEDGAQENTFSMDPQLERQVETIRNLVDSYVAIINKSIRDLMPKTIMHLMINN
TKAFIHHELLAYLYSSADQSSLMEESAEQAQRRDDMLRMYHALKEALNIIGDISTSTVSTPVPPPVDDTW
LQNTSGHSPTPQRRPVSSVHPPGRPPAVRGPTPGPPLIPMPVGATSSFSAPPIPSRPGPQSVFANNDPFS
APPQIPSRPARIPPGIPPGVPRRPPPLAPARPFF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC010233
ORF Size 1364 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010233, AAH10233
RefSeq Size 1875 bp
RefSeq ORF 1364 bp
Locus ID 13430
Gene Summary Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis (PubMed:18923138). Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (PubMed:18425118).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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