Fads1 (NM_146094) Mouse Tagged ORF Clone
CAT#: MG207139
- TrueORF®
Fads1 (tGFP-tagged) - Mouse fatty acid desaturase 1 (Fads1)
ORF Plasmid: DDK
"NM_146094" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 0710001O03Rik; A930006B21Rik; AI317215; DSD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207139 representing NM_146094
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGACCCGGTGCCGACCCCTGGCCCGGCCTCCGCCCAGCTCCGCCAAACGCGCTACTTTACTT GGGAGGAGGTGGCGCAGCGCTCCGGGCGGGAGAAGGAGCGATGGCTCGTGATCGACCGGAAGGTGTACAA CATCAGCGACTTCAGCCGCCGCCACCCGGGGGGCTCCCGGGTCATCAGCCACTACGCGGGTCAGGATGCC ACGGATCCTTTTGTGGCATTCCACATCAACAAGGGTCTTGTGAGAAAGTATATGAACTCTCTTCTGATTG GAGAGCTGGCTCCGGAGCAACCCAGCTTTGAACCCACCAAGAATAAAGCGCTAACTGATGAATTCCGGGA GCTGCGGGCCACAGTAGAGCGAATGGGCCTCATGAAGGCCAACCACCTCTTCTTCCTGGTCTACCTGCTT CACATCCTGCTGCTGGATGTGGCTGCCTGGCTCACCCTTTGGATCTTTGGAACTTCCTTGGTGCCCTTCA TCCTCTGTGCAGTACTGCTCAGTACAGTTCAGGCTCAGGCAGGTTGGCTACAGCATGACTTTGGGCACCT GTCAGTCTTTGGCACCTCGACATGGAATCACCTGCTACATCATTTTGTGATTGGCCACCTGAAGGGGGCC CCCGCCAGCTGGTGGAACCACATGCATTTCCAGCACCATGCCAAGCCTAACTGCTTCCGCAAGGACCCCG ATATCAACATGCACCCCCTCTTCTTCGCCCTGGGGAAGGTCCTTCCTGTGGAGCTCGGGAGGGAAAAGAA GAAGCACATGCCATACAACCATCAGCACAAGTACTTCTTCCTCATCGGACCCCCAGCCTTGCTGCCTCTA TACTTCCAGTGGTATATTTTCTATTTTGTGGTTCAGCGGAAGAAATGGCTGGACTTGGCCTGGATGCTCA GCTTCTATGCCCGCATCTTCTTCACTTACATGCCGCTGCTGGGGCTGAAAGGCTTCCTGGGCCTTTTCTT CATTGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTGGGTGACACAGATGAACCATATCCCCATGCACATT GATCACGACCGGAATGTGGACTGGGTCTCCACCCAGCTGCAGGCAACCTGCAACATTCACCAATCAGCCT TCAACAACTGGTTCAGTGGCCACCTAAATTTCCAGATTGAACACCACCTCTTCCCCACCATGCCGCGGCA CAACTACCACAAGGTGGCACCCCTAGTACAATCCCTGTGCGCCAAGTACGGCATCAAGTATGAGTCCAAG CCCCTGCTCACAGCCTTCGCGGACATTGTTTACTCCCTGAAGGAGTCAGGGCAACTCTGGTTGGACGCTT ACCTTCACCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207139 representing NM_146094
Red=Cloning site Green=Tags(s) MAPDPVPTPGPASAQLRQTRYFTWEEVAQRSGREKERWLVIDRKVYNISDFSRRHPGGSRVISHYAGQDA TDPFVAFHINKGLVRKYMNSLLIGELAPEQPSFEPTKNKALTDEFRELRATVERMGLMKANHLFFLVYLL HILLLDVAAWLTLWIFGTSLVPFILCAVLLSTVQAQAGWLQHDFGHLSVFGTSTWNHLLHHFVIGHLKGA PASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLPVELGREKKKHMPYNHQHKYFFLIGPPALLPL YFQWYIFYFVVQRKKWLDLAWMLSFYARIFFTYMPLLGLKGFLGLFFIVRFLESNWFVWVTQMNHIPMHI DHDRNVDWVSTQLQATCNIHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESK PLLTAFADIVYSLKESGQLWLDAYLHQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_146094 |
ORF Size | 1341 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146094.1, NP_666206.1 |
RefSeq Size | 3408 bp |
RefSeq ORF | 1344 bp |
Locus ID | 76267 |
UniProt ID | Q920L1 |
Gene Summary | Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (Probable). As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (PubMed:22534642). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204525 | Fads1 (untagged) - Mouse fatty acid desaturase 1 (Fads1), (10ug) |
CNY 3,656.00 |
|
MR207139 | Fads1 (Myc-DDK-tagged) - Mouse fatty acid desaturase 1 (Fads1) |
CNY 3,656.00 |
|
MR207139L3 | Lenti ORF clone of Fads1 (Myc-DDK-tagged) - Mouse fatty acid desaturase 1 (Fads1) |
CNY 6,056.00 |
|
MR207139L4 | Lenti ORF clone of Fads1 (mGFP-tagged) - Mouse fatty acid desaturase 1 (Fads1) |
CNY 5,230.00 |