Zic1 (BC063247) Mouse Tagged ORF Clone
CAT#: MG207138
- TrueORF®
Zic1 (tGFP-tagged) - Mouse zinc finger protein of the cerebellum 1 (cDNA clone MGC:62379 IMAGE:5694793)
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CNY 3,710.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ZIC, ZNF201 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207138 representing BC063247
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTGGACGCCGGACCCCAGTATCCCGCGATTGGTGTGACCACCTTTGGTGCATCCCGGCACCACT CGGCGGGCGACGTGGCCGAGAGAGACGTGGGCCTGGGTATCAACCCGTTCGCCGACGGCATGGGCGCCTT CAAGCTCAACCCCAGTTCGCACGAACTGGCCTCGGCTGGCCAGACAGCCTTCACGTCGCAGGCTCCGGGC TACGCCGCTGCTGCGGCCCTGGGCCACCATCACCACCCCGGCCACGTCGGCTCCTACTCCAGCGCTGCTT TCAATTCTACCCGGGACTTTCTGTTCCGCAACCGGGGCTTCGGCGACGCGGCGGCGGCAGCTAGCGCCCA GCACAGTCTCTTCGCTGCTTCGGCCGGCGGCTTTGGGGGCCCACACGGCCATACGGACGCCGCGGGCCAC CTCCTTTTTTCTGGGCTTCACGAGCAGGCGGCTGGCCACGCTTCGCCCAACGTGGTTAACGGGCAGATGC GGCTAGGTTTCTCCGGGGACATGTACCCACGGCCGGAACAGTACGGCCAGGTGACCAGCCCGCGATCCGA GCACTATGCTGCCCCGCAGCTTCACGGCTATGGGCCCATGAACGTGAACATGGCTGCACATCACGGGGCT GGAGCCTTCTTCCGCTATATGCGCCAACCCATCAAGCAAGAGCTTATCTGTAAATGGATCGAGCCGGAGC AGCTGGCCAACCCCAAAAAGTCGTGCAACAAAACTTTCAGCACCATGCACGAGCTGGTCACGCACGTCAC GGTGGAGCACGTCGGCGGCCCAGAGCAGAGCAACCACATCTGCTTCTGGGAGGAGTGTCCACGCGAGGGC AAGCCCTTCAAAGCCAAATACAAACTGGTCAACCACATCCGAGTGCACACAGGCGAAAAGCCTTTTCCCT GCCCGTTTCCTGGCTGCGGCAAGGTTTTCGCGCGTTCAGAGAACCTCAAGATCCACAAAAGGACACACAC AGGGGAGAAGCCCTTCAAGTGCGAGTTCGAGGGCTGCGACCGGCGCTTCGCCAACAGCAGCGACCGCAAG AAGCACATGCACGTGCACACGAGCGACAAGCCCTACCTTTGCAAGATGTGCGATAAGTCCTACACGCACC CCAGCTCTCTGCGTAAACACATGAAGGTCCATGAGTCCTCTTCTCAGGGCTCACAGCCTTCGCCCGCCGC CAGCTCGGGGTATGAGTCGTCCACGCCACCCACCATCGTGTCTCCCACAACAGACAACCCGACCACCAGC TCCATGTCGCCCTCCTCCTCCGCGGTTCACCACACAGCCGGCCACAGCGCGCTCTCTTCCAATTTTAACG AATGGTACGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207138 representing BC063247
Red=Cloning site Green=Tags(s) MLLDAGPQYPAIGVTTFGASRHHSAGDVAERDVGLGINPFADGMGAFKLNPSSHELASAGQTAFTSQAPG YAAAAALGHHHHPGHVGSYSSAAFNSTRDFLFRNRGFGDAAAAASAQHSLFAASAGGFGGPHGHTDAAGH LLFSGLHEQAAGHASPNVVNGQMRLGFSGDMYPRPEQYGQVTSPRSEHYAAPQLHGYGPMNVNMAAHHGA GAFFRYMRQPIKQELICKWIEPEQLANPKKSCNKTFSTMHELVTHVTVEHVGGPEQSNHICFWEECPREG KPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRK KHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSSQGSQPSPAASSGYESSTPPTIVSPTTDNPTTS SMSPSSSAVHHTAGHSALSSNFNEWYV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC063247 |
ORF Size | 1343 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC063247, AAH63247 |
RefSeq Size | 3087 bp |
RefSeq ORF | 1343 bp |
Locus ID | 22771 |
Gene Summary | Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.[UniProtKB/Swiss-Prot Function] |
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