Zic1 (BC063247) Mouse Tagged ORF Clone

CAT#: MG207138

  • TrueORF®

Zic1 (tGFP-tagged) - Mouse zinc finger protein of the cerebellum 1 (cDNA clone MGC:62379 IMAGE:5694793)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms ZIC, ZNF201
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207138 representing BC063247
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCTGGACGCCGGACCCCAGTATCCCGCGATTGGTGTGACCACCTTTGGTGCATCCCGGCACCACT
CGGCGGGCGACGTGGCCGAGAGAGACGTGGGCCTGGGTATCAACCCGTTCGCCGACGGCATGGGCGCCTT
CAAGCTCAACCCCAGTTCGCACGAACTGGCCTCGGCTGGCCAGACAGCCTTCACGTCGCAGGCTCCGGGC
TACGCCGCTGCTGCGGCCCTGGGCCACCATCACCACCCCGGCCACGTCGGCTCCTACTCCAGCGCTGCTT
TCAATTCTACCCGGGACTTTCTGTTCCGCAACCGGGGCTTCGGCGACGCGGCGGCGGCAGCTAGCGCCCA
GCACAGTCTCTTCGCTGCTTCGGCCGGCGGCTTTGGGGGCCCACACGGCCATACGGACGCCGCGGGCCAC
CTCCTTTTTTCTGGGCTTCACGAGCAGGCGGCTGGCCACGCTTCGCCCAACGTGGTTAACGGGCAGATGC
GGCTAGGTTTCTCCGGGGACATGTACCCACGGCCGGAACAGTACGGCCAGGTGACCAGCCCGCGATCCGA
GCACTATGCTGCCCCGCAGCTTCACGGCTATGGGCCCATGAACGTGAACATGGCTGCACATCACGGGGCT
GGAGCCTTCTTCCGCTATATGCGCCAACCCATCAAGCAAGAGCTTATCTGTAAATGGATCGAGCCGGAGC
AGCTGGCCAACCCCAAAAAGTCGTGCAACAAAACTTTCAGCACCATGCACGAGCTGGTCACGCACGTCAC
GGTGGAGCACGTCGGCGGCCCAGAGCAGAGCAACCACATCTGCTTCTGGGAGGAGTGTCCACGCGAGGGC
AAGCCCTTCAAAGCCAAATACAAACTGGTCAACCACATCCGAGTGCACACAGGCGAAAAGCCTTTTCCCT
GCCCGTTTCCTGGCTGCGGCAAGGTTTTCGCGCGTTCAGAGAACCTCAAGATCCACAAAAGGACACACAC
AGGGGAGAAGCCCTTCAAGTGCGAGTTCGAGGGCTGCGACCGGCGCTTCGCCAACAGCAGCGACCGCAAG
AAGCACATGCACGTGCACACGAGCGACAAGCCCTACCTTTGCAAGATGTGCGATAAGTCCTACACGCACC
CCAGCTCTCTGCGTAAACACATGAAGGTCCATGAGTCCTCTTCTCAGGGCTCACAGCCTTCGCCCGCCGC
CAGCTCGGGGTATGAGTCGTCCACGCCACCCACCATCGTGTCTCCCACAACAGACAACCCGACCACCAGC
TCCATGTCGCCCTCCTCCTCCGCGGTTCACCACACAGCCGGCCACAGCGCGCTCTCTTCCAATTTTAACG
AATGGTACGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207138 representing BC063247
Red=Cloning site Green=Tags(s)

MLLDAGPQYPAIGVTTFGASRHHSAGDVAERDVGLGINPFADGMGAFKLNPSSHELASAGQTAFTSQAPG
YAAAAALGHHHHPGHVGSYSSAAFNSTRDFLFRNRGFGDAAAAASAQHSLFAASAGGFGGPHGHTDAAGH
LLFSGLHEQAAGHASPNVVNGQMRLGFSGDMYPRPEQYGQVTSPRSEHYAAPQLHGYGPMNVNMAAHHGA
GAFFRYMRQPIKQELICKWIEPEQLANPKKSCNKTFSTMHELVTHVTVEHVGGPEQSNHICFWEECPREG
KPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRK
KHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSSQGSQPSPAASSGYESSTPPTIVSPTTDNPTTS
SMSPSSSAVHHTAGHSALSSNFNEWYV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC063247
ORF Size 1343 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC063247, AAH63247
RefSeq Size 3087 bp
RefSeq ORF 1343 bp
Locus ID 22771
Gene Summary Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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