United States
Germany
Japan
United Kingdom
China
Mid1 (BC053704) Mouse Tagged ORF Clone
CAT＃: MG207113
- TrueORF®
Mid1 (tGFP-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736)
ORF Plasmid: DDK
"BC053704" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Fxy, 61B3-R, Trim18, MGC60591 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207113 representing BC053704
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCCCAGCCAGCGAGGTCTA GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATTATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCGACACCATGTCCTCTGCCGAGAAGGT TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGCAAAACTTGGAGAG TAATCTCACCAATCTTATTAAGAGAAACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGT CAACATGTTGAAGTCAATGCATCCCGTCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAA TCATTCAGCAACGAAGACAAATTATTGGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTT AGCTCAGCAGATTGCAAACTGTAAACAGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCAC TCGCTGAAGGAAAATGACCACGCCCGTTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGG CAACTGCATCCTCCCAGGTCCTAATTCCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGA TTTTTCCCGGGAGAAGAAACTGCTAGAATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGA GAAGAGCTCTGCACCGCTTCCTACGACACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGG TCTCCTACGAGCTCCAGTACACCATATTCACCGGACAAGCCAATGTTGTCAGTCTGTGTAACTCGGCGGA CAGCTGGATGATCGTGCCCAACATCAAGCAGAACCACTACACCGTGTACGGCCTGCAAAGTGGCACCAAG TATATCTTCACGGTGAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGAAAGCTGAAGACCA ACAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207113 representing BC053704
Red=Cloning site Green=Tags(s) METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITPSQRGL DGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDADTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVS YCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTC QHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVIRLRKLAQQIANCKQCLERSASLISQAEH SLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPAIR EELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVYGLQSGTK YIFTVKAINQAGSRSSEPGKLKTNS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053704 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053704, AAH53704 |
| RefSeq Size | 3457 bp |
| RefSeq ORF | 1337 bp |
| Locus ID | 17318 |
| Gene Summary | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217722 | Mid1 (untagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736), (10ug) |
CNY 6,940.00 |
|
| MR207113 | Mid1 (Myc-DDK-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) |
CNY 3,656.00 |
|
| MR207113L3 | Lenti ORF clone of Mid1 (Myc-DDK-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) |
CNY 4,470.00 |
|
| MR207113L4 | Lenti ORF clone of Mid1 (mGFP-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) |
CNY 4,470.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
