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Esrp1 (NM_194055) Mouse Tagged ORF Clone
CAT＃: MG207090
- TrueORF®
Esrp1 (tGFP-tagged) - Mouse RNA binding motif protein 35A (Rbm35a)
ORF Plasmid: DDK
"NM_194055" in other vectors (4)
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CNY 5,256.00
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2210008M09Rik; A630065D16; BC031468; Rbm35a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207090 representing NM_194055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGAAAGCCTGAACTTTGAGAAGAGTGACTCTGTGTCCCGATACGGAGCCTCCCAAGTTGAAGACA TGGGGAACATAATTTTAGCAATGATCTCCGAGCCCTACAATCACAGATTTTCAGATCCAGAGAGATTAAA CTACAAGTTTGAAAGTGGCACTTGCAGCAAGATGGAACTTATTGATGACAGCACAGTGGTGAGAGCTCGA GGTTTGCCCTGGCAATCTTCAGATCAAGATATTGCAAGGTTCTTCAAAGGACTCAATATTGCCAAGGGTG GCGCAGCACTTTGCCTGAATGCTCAGGGCCGGAGGAACGGAGAAGCTCTGGTTAGATTTGTAAGTGAGGA GCACAGAGATCTAGCCCTGCAGAGGCACAAACATCACATGGGGACCCGGTACATTGAGGTTTACAAAGCA ACAGGCGAAGATTTCCTTAAAATTGCCGGAGGGACATCCAATGAGGTAGCCCAGTTTCTCTCCAAGGAAA ACCAAGTCATCGTGCGCATGAGGGGGCTCCCTTTCACGGCCACGGCTGAAGAAGTGGTGGCCTTCTTTGG ACAGCACTGCCCCATCACTGGAGGGAAGGAAGGCATCCTCTTCGTTACCTACCCAGATGGTAGGCCAACC GGAGATGCTTTTGTGCTCTTTGCTTGTGAGGAGTATGCTCAGAACGCCCTGAGGAAGCACAAGGAGTTGC TGGGTAAAAGATACATCGAGCTCTTCAGGAGCACAGCTGCCGAAGTTCAGCAGGTGCTGAACCGATTCTC CTCAGCCCCTCTGATTCCACTTCCAACCCCTCCCATTATCCCTGTACTACCTCAGCAGTTTGTGCCTCCC ACGAATGTCAGAGACTGCATACGTCTGCGAGGTCTTCCCTATGCTGCCACAATAGAAGACATTCTGGACT TCCTGGGGGAGTTTTCCACAGATATTCGTACACATGGAGTGCACATGGTATTGAATCACCAGGGACGCCC GTCAGGAGATGCCTTTATCCAGATGAAGTCAACAGACAGAGCATTCATGGCTGCGCAGAAGTACCATAAA AAAACCATGAAGGACAGATATGTTGAAGTCTTTCAGTGTTCAGCTGAGGAGATGAACTTTGTGTTAATGG GGGGCACTTTAAATCGAAATGGCTTGTCCCCACCGCCATGCCTGTCTCCTCCCTCCTACACATTCCCAGC TCCCACGGCTGTGATTCCTACCGAAGCTGCCATTTATCAACCTTCTCTGCTCTTGAACCCACGGGCACTG CAGCCCTCCACTGCATACTACCCGGCAGGCACTCAGCTCTTCATGAACTACACAGCCTACTATCCCAGTG TT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207090 representing NM_194055
Red=Cloning site Green=Tags(s) MTESLNFEKSDSVSRYGASQVEDMGNIILAMISEPYNHRFSDPERLNYKFESGTCSKMELIDDSTVVRAR GLPWQSSDQDIARFFKGLNIAKGGAALCLNAQGRRNGEALVRFVSEEHRDLALQRHKHHMGTRYIEVYKA TGEDFLKIAGGTSNEVAQFLSKENQVIVRMRGLPFTATAEEVVAFFGQHCPITGGKEGILFVTYPDGRPT GDAFVLFACEEYAQNALRKHKELLGKRYIELFRSTAAEVQQVLNRFSSAPLIPLPTPPIIPVLPQQFVPP TNVRDCIRLRGLPYAATIEDILDFLGEFSTDIRTHGVHMVLNHQGRPSGDAFIQMKSTDRAFMAAQKYHK KTMKDRYVEVFQCSAEEMNFVLMGGTLNRNGLSPPPCLSPPSYTFPAPTAVIPTEAAIYQPSLLLNPRAL QPSTAYYPAGTQLFMNYTAYYPSV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_194055 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_194055.1, NP_918944.1 |
| RefSeq Size | 3012 bp |
| RefSeq ORF | 2046 bp |
| Locus ID | 207920 |
| UniProt ID | Q3US41 |
| Gene Summary | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (By similarity). Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (PubMed:29107558).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204512 | Esrp1 (untagged) - Mouse epithelial splicing regulatory protein 1 (Esrp1), (10ug) |
CNY 3,656.00 |
|
| MR207090 | Esrp1 (Myc-DDK-tagged) - Mouse epithelial splicing regulatory protein 1 (Esrp1) |
CNY 3,656.00 |
|
| MR207090L3 | Lenti ORF clone of Esrp1 (Myc-DDK-tagged) - Mouse epithelial splicing regulatory protein 1 (Esrp1) |
CNY 7,700.00 |
|
| MR207090L4 | Lenti ORF clone of Esrp1 (mGFP-tagged) - Mouse epithelial splicing regulatory protein 1 (Esrp1) |
CNY 7,700.00 |
