Spred1 (BC079606) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730461F13Rik; AL024345; AW047647
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207088 representing BC079606 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCA GAGATGACTCAAGTGGTGGATGGCTACCACTCGGAGGGAGCGGACTGAGCAGCGTCACTGTCTTCAGAGT CCCTCATCAGGAAGAGAATGGCTGTGCGGACTTTTTTATCCGTGGAGAGAGACTCAGAGACAAAATGGTG GTTTTGGAATGTATGCTTAAGAAAGACCTCATCTATAATAAGGTCACTCCCACATTTCACCACTGGAAGA TCGATGACAAGAAGTTTGGCCTTACCTTTCAGAGTCCTGCTGATGCCAGGGCTTTTGATCGAGGCATTCG AAGAGCTATAGAGGATATATCTCTAGGGTGCCCAGCGTCAAAAACTGAGGCTGAAGGAGGAGATGATGAT TTACAAACGACTGAAGAAGACACTTCCCGTTCCCTAGTGAAAGATCACTTTTTCCAGCAAGAGACAGTTG TTACCAGTGAACCTTACAGAAGCTCAGACATAAGACCGTTACCCTTTGAAGATCTGAATGCCAGAAGAGT CTACTTGCAAAGCCAAGTCAGCCAGATACCATTCAGTCAGCAAGGCCTAGACATTCAGAGCCGAAGTATG GAATATGTACAGCGGCAAATATCTAAGGAATGTGGGAGCCTAAAGTCCCAAACTAGGGTCCCTTTGAAGT CAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTTAGAATAAATCCTCGAGATATCTTAATACG TCGATATGCAGACTACAGACATCCGGACATGTGGAAAAATGACTTGGAGAGAGATGATACTGACTCCAGT GTTCCATTTTCTAAACAAGACAGTAAAAAGTCAGACTATCTCTACCACTGTGGGGATGAGACTAAGTTAA GTTCTCTGAAAGACTCTGTGGTATTTAAGACACAGCCTCCCTCGTTAAAATTTAAGTCAAAACGAAGAAA AGAGGATGGGGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAACGCCAGGGGG AAATGCCAGGACGCTCCAGATCCTGTTAAAAGATGCATATATCAAGTTAGCTGCATGCTCTGTGCTGAGA GCATGCTATATCATTGTATGTCAGACTCAGAGGGAGACTTTTCTGATCCTTGTTCGTGTGACACTAGCGA TGACAAGTTCTGCCTAAGGTGGTTAGCCCTGGTAGCTCTGTCTTTCATTGTACCATGTATGTGCTGCTAC GTCCCTTTGAGAATGTGCCATCGCTGTGGTGAGGCATGCGGGTGCTGTGGTGGGAAACATAAGGCTGCTG GG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207088 representing BC079606 Red=Cloning site Green=Tags(s) MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFRVPHQEENGCADFFIRGERLRDKMV VLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISLGCPASKTEAEGGDDD LQTTEEDTSRSLVKDHFFQQETVVTSEPYRSSDIRPLPFEDLNARRVYLQSQVSQIPFSQQGLDIQSRSM EYVQRQISKECGSLKSQTRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDTDSS VPFSKQDSKKSDYLYHCGDETKLSSLKDSVVFKTQPPSLKFKSKRRKEDGERSRCVYCQERFNHEENARG KCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCY VPLRMCHRCGEACGCCGGKHKAAG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC079606
ORF Size	1334 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079606, AAH79606
RefSeq Size	3042 bp
RefSeq ORF	1334 bp
Locus ID	114715
Gene Summary	This gene encodes a membrane-associated protein that is phosphorylated by tyrosine kinases in response to growth factors. The encoded protein acts as a negative regulator of the mitogen-activated protein (MAP) kinase signaling pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

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