Nfix (BC003766) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CTF; NF-I/X; NF1-X; NFI-X
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207035 representing BC003766 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTCCCCGTACTGCCTCACCCAGGATGAGTTCCACCCGTTTATCGAGGCGCTGCTGCCTCACGTCC GAGCCTTCTCCTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCACGAGAAGCG GATGTCAAAGGACGAGGAGCGCGCAGTGAAGGACGAGCTGCTGGGCGAGAAGCCTGAGATCAAGCAGAAG TGGGCATCCCGGCTGTTGGCCAAGCTGCGCAAAGACATCCGGCCCGAGTTCCGCGAGGACTTTGTGCTAA CCATCACGGGCAAGAAGCCCCCCTGCTGCGTGCTTTCCAACCCCGACCAGAAGGGCAAGATCCGGCGGAT TGACTGCCTGCGCCAGGCTGACAAGGTGTGGCGGCTGGACCTGGTCATGGTGATTTTGTTTAAAGGGATC CCTTTGGAAAGTACTGATGGGGAGCGGCTCTACAAGTCGCCCCAGTGCTCGAACCCCGGCCTGTGTGTCC AGCCACATCACATTGGAGTCACAATCAAAGAACTGGACCTTTATCTGGCTTACTTTGTCCACACTCCGGA ATCCGGACAATCAGATAGTTCAAACCAGCAAGGAGATGCGGACATCAAACCACTGCCCAACGGGCACTTA AGTTTCCAGGACTGCTTTGTGACGTCTGGGGTCTGGAATGTGACAGAGCTGGTGAGAGTATCACAGACTC CAGTTGCGACTGCATCAGGGCCCAACTTCTCACTGGCGGACCTGGAGAGCCCCAGCTACTACAACATAAA TCAAGTGACCCTGGGAAGGCGGTCCATCACCTCCCCTCCTTCCACCAGCAGCACCAAGCGCCCCAAGTCC ATCGACGACAGTGAGATGGAGAGTCCAGTAGATGATGTGTTCTATCCTGGGACAGGCCGCTCTCCGGCCG CTGGCAGCAGCCAGTCTAGCGGATGGCCCAATGACGTGGATGCAGGCCCTGCTTCTCTAAAGAAGTCAGG AAAGCTGGACTTCTGCAGCGCCCTCTCCTCTCAGGGCAGTTCCCCACGCATGGCTTTCACCCACCACCCG CTGCCTGTGCTTGCTGGAGTCAGACCAGGGAGCCCCCGGGCCACGGCATCAGCGCTGCACTTCCCTTCCA CGTCCATCATCCAGCAGTCGAGCCCGTATTTCACACACCCAACCATCCGCTACCACCACCACCATGGGCA GGACTCGCTGAAGGAGTTTGTGCAGTTTGTGTGCTCTGACGGCTCGGGTCAGGCCACCGGACAGCATTCA CAACGACAGGCGCCTCCTCTGCCAACCGGTTTGTCAGCATCGGATCCCGGGACGGCAACTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207035 representing BC003766 Red=Cloning site Green=Tags(s) MYSPYCLTQDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEIKQK WASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGI PLESTDGERLYKSPQCSNPGLCVQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHL SFQDCFVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSSTKRPKS IDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHP LPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQHS QRQAPPLPTGLSASDPGTATF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC003766
ORF Size	1325 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003766, AAH03766
RefSeq Size	1444 bp
RefSeq ORF	1325 bp
Locus ID	18032
Gene Summary	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.[UniProtKB/Swiss-Prot Function]

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