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Gtf2e1 (NM_028812) Mouse Tagged ORF Clone
CAT＃: MG207009
- TrueORF®
Gtf2e1 (tGFP-tagged) - Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1)
ORF Plasmid: DDK
"NM_028812" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 56kDa; 2610024P03Rik; AI303800; FE; TFIIE-A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207009 representing NM_028812
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCCAGATGTTCTCACTGAAGTTCCGGCGGCATTGAAGCGGCTAGCCAAGTATGTCATCCGGG GCTTTTATGGCATTGAGCACGTGTTGGCCTTGGACATCTTGATCCGGAACCCCTGTGTAAAAGAAGAGGA CATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGATCCGTTTTGAATAATTTAAAGGGAGACAAG TTCATCAAATGCAGAATGAGGGTAGAGACTGCTGCAGATGGGAAGACCACCCGCCATAACTACTACTTCA TCAATTACCGCACTCTTGTTAATGTAGTGAAATATAAGCTGGACCACATGAGAAGAAGGATTGAAACAGA TGAGAGAAATTCCACCAATCGGGCTTCCTTCAAATGTCCTGTCTGCTGCAGCACTTTCACTGACCTAGAA GCCAATCAACTGTTTGATCCCATGACAGGAACTTTCCGCTGCACCTTTTGCCATACAGAGGTAGAAGAAG ATGAATCAGCAATGCCCAAAAAAGATGCTCGCACGCTTTTGGCAAGATTTAATGAACAAATTGAGCCCAT TTATGCATTGCTTCGGGAGACAGAGGATGTAAACTTGGCCTATGAAATACTTGAACCAGAACCCACAGAA ATCCCAGCACTGAAGCAAAGCAAGGACCGAGCAGCAACTGCTGCTGGAGCTGCTGGCCTGGCAGGTGGGC ACCACCGAGAGGCGTGGACCAACAAAGGCCCCTCGTATGAGGACTTGTATACTCAGAATGTTGTCATTAA CATGGATGATCAAGATGATGTGCATCGCCCCTCGCTGGAAGGGAAAGCTGCCAAAGAGAGACCCATTTGG TTGAGAGAGAGCACTGTCCAAGGGGCATACAGTTCTGAAGAGATGAAGGAAGGTGGCATCGATGTGGACA CATTTCAGGAGCGCGAGGAAGCCCGTGCGGGGCCTGATGACAATGAGGAGGTCATGCGAGCTCTACTCAT TCATGAGAAGAAGACTTCCTCGGTGACAGCTGGCTCTGTGGGAGCAGCCGCTCCGGTGACAGCTGCTAAT GGCAGTGACTCTGAGAGTGAGACCAGCGAGTCAGATGATGACTCTCCACCTCGGCCAGCAGCTGCGGCTC CTCCTCATCATCATCGCGATGAAGATGAGGAAGACGAGGAGTTTGAGGAAGTAGCAGATGACCCCATTGT TATGGTGGCTGGCTGTCCATTTTCCTACAGTGAAGTTAGCCAGCGGCCGGAGCTGGTGGCCCAGATGACC CCAGAAGAGAAGGAAGCATACATAGCCATGGGCCAGCGTATGTTTGAGGATCTCTTTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207009 representing NM_028812
Red=Cloning site Green=Tags(s) MADPDVLTEVPAALKRLAKYVIRGFYGIEHVLALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDK FIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERNSTNRASFKCPVCCSTFTDLE ANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTE IPALKQSKDRAATAAGAAGLAGGHHREAWTNKGPSYEDLYTQNVVINMDDQDDVHRPSLEGKAAKERPIW LRESTVQGAYSSEEMKEGGIDVDTFQEREEARAGPDDNEEVMRALLIHEKKTSSVTAGSVGAAAPVTAAN GSDSESETSESDDDSPPRPAAAAPPHHHRDEDEEDEEFEEVADDPIVMVAGCPFSYSEVSQRPELVAQMT PEEKEAYIAMGQRMFEDLFE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028812 |
| ORF Size | 1320 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028812.4 |
| RefSeq Size | 3060 bp |
| RefSeq ORF | 1323 bp |
| Locus ID | 74197 |
| UniProt ID | Q9D0D5 |
| Gene Summary | Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203488 | Gtf2e1 (untagged) - Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1), (10ug) |
CNY 3,656.00 |
|
| MR207009 | Gtf2e1 (Myc-DDK-tagged) - Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1) |
CNY 3,656.00 |
|
| MR207009L3 | Lenti ORF clone of Gtf2e1 (Myc-DDK-tagged) - Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1) |
CNY 5,230.00 |
|
| MR207009L4 | Lenti ORF clone of Gtf2e1 (mGFP-tagged) - Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1) |
CNY 5,230.00 |
