Fbxo9 (BC020074) Mouse Tagged ORF Clone

CAT#: MG206949

  • TrueORF®

Fbxo9 (tGFP-tagged) - Mouse f-box protein 9 (cDNA clone MGC:28077 IMAGE:3710611)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 9030401P18Rik; AA986398
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206949 representing BC020074
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGCGGAGGAAGATTGTCATTCTGATGCTGACAGAGTAGGCGATGAAGGAAATGAGAGTCCGG
CTGAGAGAGACCTGCAGGCGCAGCTCCAGATGTTCAGAGCTCAGTGGATGTTTGAACTGACCCCAGGTGT
AGGCTCCAGTCATGGAGAAACTCGGCCTTGCAGAGCAGGAAGAAGCTCTATGCTGAAAGCAGCTGCAGAC
ACCAAAGGACGACAGGAACTGGCAAAGGAAGAAAAGGCTCGAGAACTCTTCCTGCAGGCAGTGGAGGAAG
AACAAAATGGAGCTCTCTATGAAGCCATCAAGTTCTACCGTAGGGCGATGCAGCTGGTGCCAGACATTGA
GTTCAAGATCACTTACACCCGGTCTCCAGACGGCGACGGCGTTGGGAGCGGCTACATCGAAGAGAACGAG
GACGCCAGCAAGATGGCCGATCTCCTGTCGTACTTCCAGCAGCAGCTCACGTTGCAGGAGTCTGTGCTCA
AACTCTGTCAGCCTGAGCTTGAGACCAGTCAGACTCACATATCAGTCCTGCCTATGGAGGTGCTGATGTA
CATCTTCCGATGGGTGGTATCAAGTGACTTGGACCTCAGATCGTTAGAGCAGTTGTCACTGGTGTGCAGA
GGATTCTATATCTGTGCCAGAGACCCTGAAATCTGGCGTCTGGCTTGCTTGAAAGTGTGGGGCAGAAGCT
GCATGAAGCTCGTTCCATACGCGTCCTGGAGAGAGATGTTTCTAGAACGGCCCCGAGTTCGGTTTGATGG
AGTGTACATCAGTAAAACCACGTATATTCGCCAGGGAGAGCAGTCACTTGATGGTTTCTACAGGGCGTGG
CACCAAGTGGAATATTACAGATACATGAGATTCTTTCCTGATGGCCATGTGATGATGTTAACCACCCCCG
AGGAGCCTCCGTCCATCGTTCCCCGGTTAAGAACCAGGAACACCAGAACGGATGCAATTCTGCTGGGTCA
TTACCGCTTGTCACAAGATGCAGACAATCAGACCAAAGTCTTTGCTGTAATAACTAAGAAAAAAGAAGAA
AAGCCACTTGACCATAAATACAGGTATTTTCGTCGTGTTCCTGTCCAAGAGGCAGATCACAGCTTCCATG
TGGGACTGCAGCTGTGCTCCAGTGGCCACCAGAGGTTCAACAAACTCATCTGGATCCACCACTCTTGTCA
CATCACTTACAAAGCAACTGGTGAGACTGCAGTGAGTGCTTTTGAGATTGACAAGATGTACACGCCCCTG
TTGTTCGCCAGAGTGAGGAGCTACACTGCCTTCTCGGAAAGGCCTCTG


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206949 representing BC020074
Red=Cloning site Green=Tags(s)

MAEAEEDCHSDADRVGDEGNESPAERDLQAQLQMFRAQWMFELTPGVGSSHGETRPCRAGRSSMLKAAAD
TKGRQELAKEEKARELFLQAVEEEQNGALYEAIKFYRRAMQLVPDIEFKITYTRSPDGDGVGSGYIEENE
DASKMADLLSYFQQQLTLQESVLKLCQPELETSQTHISVLPMEVLMYIFRWVVSSDLDLRSLEQLSLVCR
GFYICARDPEIWRLACLKVWGRSCMKLVPYASWREMFLERPRVRFDGVYISKTTYIRQGEQSLDGFYRAW
HQVEYYRYMRFFPDGHVMMLTTPEEPPSIVPRLRTRNTRTDAILLGHYRLSQDADNQTKVFAVITKKKEE
KPLDHKYRYFRRVPVQEADHSFHVGLQLCSSGHQRFNKLIWIHHSCHITYKATGETAVSAFEIDKMYTPL
LFARVRSYTAFSERPL

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC020074
ORF Size 1310 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC020074, AAH20074
RefSeq Size 2138 bp
RefSeq ORF 1310 bp
Locus ID 71538
Gene Summary Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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