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Pnkd (BC058945) Mouse Tagged ORF Clone
CAT＃: MG206764
- TrueORF®
Pnkd (tGFP-tagged) - Mouse paroxysmal nonkinesiogenic dyskinesia (cDNA clone MGC:66659 IMAGE:6812127)
ORF Plasmid: DDK
"BC058945" in other vectors (4)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Tahccp2, MR-1, MNCb-5687 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206764 representing BC058945
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGTGGTAGCTGCTACGGCGCTGAAGGGCCGGGGGGCGAGAAATGCCCGCGTCCTCCGGGGGA TCCTCTCTGGAGCCACAGCTAACAAGGCTTCCCAGAACAGGACCAGAGCACTGCAGAGCCACAGCTCACC AGAATGCAAGGAGGAGCCCGAGCCCCTCTCCCCTGAGCTAGAATACATTCCCAGAAAGAGGGGCAAGAAC CCCATGAAAGCCGTGGGGCTAGCCTGGGCCATCGGCTTCCCCTGTGGTATCCTCTTCTTCGTCCTCACCA AGCAGGAAGTGGACAAGGACCGATTGAAGCAGATGAAAGCTCGTCAGAACATGCGGGTGTCCAACACGGG CGAGTATAGCCTGTACACCCGAACCTGGCTTGGGTACCTCTTCTACCGACAGCAGCTTCGGAGGGCTCGG AATCGCTATCCCAAAGGCCACTCCAAAACTCAGCCCCGCCTCTTCAATGGAGTGAAGGTGCTTCCCATCC CTGTCCTCTCGGACAACTACAGCTACCTCATCATCGACACCCAGGCTGGGCTGGCAGTGGCTGTGGACCC CTCAGACCCGAGGGCTGTGCAGGCTTCCATTGAAAAGGAAAGAGTTAACTTGGTTGCCATTCTCTGCACC CACAAGCACTGGGACCACAGTGGAGGGAACCGTGACCTCAGCCGGCGGCACAGGGACTGTCGAGTGTATG GGAGCCCTCAGGATGGTATCCCCTACCTCACCCACCCCCTGTGTCATCAAGATGTGGTCAGCGTGGGACG ACTTCAGATTCGAGCCCTAGCCACCCCTGGCCACACTCAAGGCCATCTGGTCTACCTGCTGGATGGGGAG CCCTACAAAGGTCCTTCCTGCCTCTTCTCAGGGGACCTGCTCTTCCTCTCTGGCTGTGGACGGACCTTCG AAGGCACAGCGGAGACCATGCTGAGCTCACTGGACACTGTGTTAGACCTGGGGGATGACACCCTGCTGTG GCCTGGGCATGAATACGCAGAAGAGAACCTGGGCTTTGCAGGCGTGGTGGAGCCCGAGAACTTGGCTCGT GAGAGGAAGATGCAGTGGGTACAGAGGCAACGGATGGAACGCAAGAGCACATGCCCATCCACCCTGGGAG AGGAACGCGCCTACAACCCATTCCTGAGAACCCATTGCCTGGAACTACAGGAGGCTCTGGGGCCAGGGCC AGGCCCCACCAGTGATGACGGCTGCTCCCGGGCCCAGCTCCTGGAGGAGCTGCGCCGCCTGAAGGACATG CATAAAAGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206764 representing BC058945
Red=Cloning site Green=Tags(s) MAAVVAATALKGRGARNARVLRGILSGATANKASQNRTRALQSHSSPECKEEPEPLSPELEYIPRKRGKN PMKAVGLAWAIGFPCGILFFVLTKQEVDKDRLKQMKARQNMRVSNTGEYSLYTRTWLGYLFYRQQLRRAR NRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAGLAVAVDPSDPRAVQASIEKERVNLVAILCT HKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGE PYKGPSCLFSGDLLFLSGCGRTFEGTAETMLSSLDTVLDLGDDTLLWPGHEYAEENLGFAGVVEPENLAR ERKMQWVQRQRMERKSTCPSTLGEERAYNPFLRTHCLELQEALGPGPGPTSDDGCSRAQLLEELRRLKDM HKSK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058945 |
| ORF Size | 1274 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058945, AAH58945 |
| RefSeq Size | 3199 bp |
| RefSeq ORF | 1274 bp |
| Locus ID | 56695 |
| Gene Summary | Probable hydrolase that plays an aggravative role in the development of cardiac hypertrophy via activation of the NF-kappa-B signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217802 | Pnkd (untagged) - Mouse paroxysmal nonkinesiogenic dyskinesia (cDNA clone MGC:66659 IMAGE:6812127), (10ug) |
CNY 6,940.00 |
|
| MR206764 | Pnkd (Myc-DDK-tagged) - Mouse paroxysmal nonkinesiogenic dyskinesia (cDNA clone MGC:66659 IMAGE:6812127) |
CNY 3,656.00 |
|
| MR206764L3 | Lenti ORF clone of Pnkd (Myc-DDK-tagged) - Mouse paroxysmal nonkinesiogenic dyskinesia (cDNA clone MGC:66659 IMAGE:6812127) |
CNY 4,280.00 |
|
| MR206764L4 | Lenti ORF clone of Pnkd (mGFP-tagged) - Mouse paroxysmal nonkinesiogenic dyskinesia (cDNA clone MGC:66659 IMAGE:6812127) |
CNY 4,280.00 |
