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Acot1 (BC028261) Mouse Tagged ORF Clone
CAT＃: MG206642
- TrueORF®
Acot1 (tGFP-tagged) - Mouse acyl-CoA thioesterase 1 (cDNA clone MGC:40635 IMAGE:5100478)
ORF Plasmid: DDK
"BC028261" in other vectors (3)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACH2, CTE-1, CTE-I |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206642 representing BC028261
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGACGCTGAACCTTGAGCCCTCGGGCCGCAGCTGCTGGGACGAACCGCTGAGCATCGCCGTGC GCGGCCTGGCCCCGGAGCAGCCCGTCACGCTGCGCTCTGTCCTGCGCGACGAGAAGGGCGCGCTCTTCCG AGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGCACGCCCGCGCTGGGCGGC AGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATCGGCCTTTTTGGCGATTGG TCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGACGGCGG GCAGCGGCTTGCACATGCGGTGCACGAGCGTCACTTCTTGGCTCCGGGGGTGCGGCGCGTGCCCGTACGA GAGGGCCGGGTGCGCGCCACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTCCTGGGATCATAGACCTTT TTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTCATGGC TCTGGCTTATTACAACTACGATGACCTCCCCAAGAACATGGAAACCATGCACATGGAGTACTTTGAAGAA GCCGTGAACTACCTGCGCAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGCTGCTTGGGATTTCCAAAG GGGGTGAACTTGGCCTTGCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGCTGTGGTCATCAATGGCTC CGTGGCTGCTGTTGGGAACACCATCTCCTACAAGGATGAGACTATACCCCCTGTGACTATCCTGAGAAAT CAGGTCAAAATGACCAAGGATGGCCTCAAGGATGTTGTAGATGCTTTGCAAAGCCCTCTGGTAGACAAGA AGAGCTTCATTCCCGTGGAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGGTCAGGACGACCACAACTG GAAGAGCGAGTTCTATGCTGATGAGATCTCCAAACGCCTGCAGGCGCACGGGAAGGAGAAGCCCCAGATC ATCTGCTACCCAGCAGCAGGGCATTACATCGAGCCCCCTTACTTCCCGCTGTGCAGCGCTGGCATGCACC TCCTGGTGGGTGCCAACATCACCTTTGGAGGGGAGCCCAAGCCTCATGCCATGGCCCAGTTGGATGCATG GCAGCAGCTCCAGACTTTCTTCCACAAACAGTTGGGTAGTGAGTGTTTGCATGTGTCTCCTAAAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206642 representing BC028261
Red=Cloning site Green=Tags(s) MEATLNLEPSGRSCWDEPLSIAVRGLAPEQPVTLRSVLRDEKGALFRAHARYRADSHGELDLARTPALGG SFSGLEPMGLLWAMEPDRPFWRLVKRDVQTPFVVELEVLDGHEPDGGQRLAHAVHERHFLAPGVRRVPVR EGRVRATLFLPPEPGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYNYDDLPKNMETMHMEYFEE AVNYLRSHPEVKGPGIGLLGISKGGELGLAMASFLKGITAAVVINGSVAAVGNTISYKDETIPPVTILRN QVKMTKDGLKDVVDALQSPLVDKKSFIPVERSDTTFLFLVGQDDHNWKSEFYADEISKRLQAHGKEKPQI ICYPAAGHYIEPPYFPLCSAGMHLLVGANITFGGEPKPHAMAQLDAWQQLQTFFHKQLGSECLHVSPKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC028261 |
| ORF Size | 1259 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC028261, AAH28261 |
| RefSeq Size | 1587 bp |
| RefSeq ORF | 1259 bp |
| Locus ID | 26897 |
| Gene Summary | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206642 | Acot1 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 1 (cDNA clone MGC:40635 IMAGE:5100478) |
CNY 3,656.00 |
|
| MR206642L3 | Lenti ORF clone of Acot1 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 1 (cDNA clone MGC:40635 IMAGE:5100478) |
CNY 4,280.00 |
|
| MR206642L4 | Lenti ORF clone of Acot1 (mGFP-tagged) - Mouse acyl-CoA thioesterase 1 (cDNA clone MGC:40635 IMAGE:5100478) |
CNY 4,280.00 |
