Cbx6 (BC048240) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206529 representing BC048240 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTCTGCAGTAGGCGAGCGGGTCTTCGCGGCCGAATCCATCATTAAACGCCGGATCCGCAAGG GACGCATCGAGTACCTGGTGAAATGGAAAGGGTGGGCGATCAAGTACAGCACTTGGGAGCCAGAGGAGAA CATTCTGGATTCGAGGCTCATCGCAGCCTTCGAGCAGAAGGAAAGGGAACGTGAGCTGTATGGGCCCAAG AAGAGGGGACCTAAACCCAAAACTTTCCTCCTAAAGGCCCGGGCCCAGGCGGAGGCCCTCCGCATCAGTG ATGTGCATTTCTCTGTCAAGCCGAGCGCCAGCGCCTCCTCGCCCAAGCTGCACTCCAGCGCTGCGGTGCA CCGGCTCAAGAAAGACATCCGCCGCTGCCACCGTATGTCTCGCCGTCCCCTGCCACGACCAGACCCACAA GGGGGTAGCCCTGGCCTACGCCCACCCATCTCGCCCTTTTCTGAGACCGTGCGCATCATCAACCGTAAGG TGAAGCCCCGGGAGCCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTGATTGACAAGGGTCCGGGAGG TGGCAGCACTGCGCAGGGCACCGGGGCACTGGCCCGCCCGAAAGTCCCCTCGCGGAACCGGGTCATTGGG AAGAGCAAGAAGTTCAGCGAGAGCATGCTCCGTACCCAGATCCGCCACATGAAGTTTGGCACATTTGCGC TCTACAAGCCCCCTCCTGCCCCTCTGGCGCCCTCTACTGCGGGCAAAGCCGATGTGGCCTCCTCGGGGCC TGGGCTGCTCCTGGCCACCCCAGCTGCTGCCCCCTTTGACGCACACAGCTCCAGCTCTTCCGGCTGCCCC TCACCCACACTGCAGTCCTCTGACCCAGACGATGCACCACCCAAGCTACTCCCGGAGACCCTGAGCCGAT CTGTGCCCAACTGGCGAGAGTCAGAGGTGCTCGATCTGTCCATCCCTCCTGAGGCGGCTGCCACCGGCCA GCGGGTGCCTCCTGATGTCACTGGTGCTGCGGACCAGGCGCTACATACAGCTCTGGAGCCCACAGGTGCT GGCTCCTCCGAGCCTGAGGCTGGGGACTGGCGCCCCGAGATGTCGCCATGCTCCAATGTAGTTGTCACAG ATGTCACCAGCAACCTCCTGACCGTGACCATCAAGGAATTCTGCAGCCCGGAGGATTTTGAGAAGGTGGC CGCTGGGGTGGCAGGTGCTACAGGGGGCGGGGGTGGCACTGGGCCAAGTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206529 representing BC048240 Red=Cloning site Green=Tags(s) MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPK KRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQ GGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGPGGGSTAQGTGALARPKVPSRNRVIG KSKKFSESMLRTQIRHMKFGTFALYKPPPAPLAPSTAGKADVASSGPGLLLATPAAAPFDAHSSSSSGCP SPTLQSSDPDDAPPKLLPETLSRSVPNWRESEVLDLSIPPEAAATGQRVPPDVTGAADQALHTALEPTGA GSSEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCSPEDFEKVAAGVAGATGGGGGTGPSK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC048240
ORF Size	1244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC048240, AAH48240
RefSeq Size	3124 bp
RefSeq ORF	1244 bp
Locus ID	494448
Gene Summary	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin (By similarity). Recruitment to chromatin might occur in an H3K27me3-independent fashion (PubMed:22226355, PubMed:16537902). May have a PRC1-independent function in embryonic stem cells (PubMed:22226355).[UniProtKB/Swiss-Prot Function]

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