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Wdr4 (BC039272) Mouse Tagged ORF Clone
CAT＃: MG206503
- TrueORF®
Wdr4 (tGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
ORF Plasmid: DDK
"BC039272" in other vectors (3)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI415180; AI448349; D530049K22Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206503 representing BC039272
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCTCTGCGGGGCTGGCACTGTGCGCCCAGACGCTGGTGGTGCGAGGAGGCAGCCGGTTCCTAG CCTTCTCCACTACGGGCAGTGATGATGACTGTGTCTTCACATACGACTGCAGTACTGCAGAGAAGAAGGC CACGCCAGAAGATAAAGGGGAGGACGGACAGCCCGCAGACACAGGGAGTGACTCGATTCTGGCGTCCACC TTCTCCAAGTCTGGCCGCTATTTTGCTTTAACAGATGACAGTAAGCGTCTGATTCTTTTCCGTACAAAAC CATGGCAATGTCTGAGTGTCAGGATGGTGGTGCGGAGGTGCACCGCCCTGACCTTCACAGCCTCAGAGGA CCGAGTCTTGGTGGCTGACAAGTCTGGAGACGTCTACTCCTTTTCGGTGCTGGAGCCAGATGGATGTGGC AGGCTGGAGCTTGGGCACCTCTCCATGCTGCTAGACGTGGCTGTGAGTCCTGATGACCAGTTTGTGCTTA CTGCAGACCGGGATGAGAAGATCCGGGTCAGCTGGGCTGCTGCCCCGCATAGCATCGAGTCTTTCTGCCT GGGACACACTGAGTTTGTGAGCCGCATCCTTGTAGTGCCCAGTCATCCTGAACTGCTGCTTTCTTCCTCT GGGGATGGCACCCTGAGACTCTGGGAGTACAGAAGCGGTAGGCAGCTGCAGTGCTGTGACCTGGCCGGCC TACAGGAGCCTGGAGAGCAGCCAGGCCACAAGGGGTTGGCCGCGTCCAGGATTGCATTCTGGGGACAGGA GAGCTATGTGGTGCTTCTGTGTGAGTGCGTTCCCGTGGTCTTCGTCTTCCAGCTTGATGCCAGCAGACAG CAGCTGGTGTTCAGACAGCGGCTGACTTTCCCTCACCGAGTGTGGGATGTTGTGTTTGAGGAGGCCCGGG GGCTGTGGGTTCTACAGGACTGCCGTGATGCCCCCCTGGTGCTCTGGAGGCCTGTGGGTGGTGAGTGGCA GGCTGCTCCAGACGGTGCTGTGTCCCCGAGACTCTGCAGCCATCTCCGTGAGAGCTGGGCCATGCTGGAA GGTTCTGTTGGTACAGATGACAGCTTCCGCAGCCTGTACAAGGCCACCTTTGACAACATGACCTCTTACC TGAAGAAAAAGGAAGAGAGACTGCAGCAGCAGCTGAAGAAGAAGCGGCAAAGGAGCCCCTTCCCAGGGTC CCCGGAACAGACCAAAAAGGCGTGCCCGGGCCAGTCAGCCCTTAGTTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206503 representing BC039272
Red=Cloning site Green=Tags(s) MASSAGLALCAQTLVVRGGSRFLAFSTTGSDDDCVFTYDCSTAEKKATPEDKGEDGQPADTGSDSILAST FSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCG RLELGHLSMLLDVAVSPDDQFVLTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSS GDGTLRLWEYRSGRQLQCCDLAGLQEPGEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQ QLVFRQRLTFPHRVWDVVFEEARGLWVLQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAMLE GSVGTDDSFRSLYKATFDNMTSYLKKKEERLQQQLKKKRQRSPFPGSPEQTKKACPGQSALSC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC039272 |
| ORF Size | 1241 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC039272, AAH39272 |
| RefSeq Size | 2054 bp |
| RefSeq ORF | 1241 bp |
| Locus ID | 57773 |
| Gene Summary | Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (By similarity). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (PubMed:29983320). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (By similarity). Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206503 | Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600) |
CNY 3,656.00 |
|
| MR206503L3 | Lenti ORF clone of Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600) |
CNY 4,280.00 |
|
| MR206503L4 | Lenti ORF clone of Wdr4 (mGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600) |
CNY 4,280.00 |
