Tank (BC026148) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C86182; E430026L09Rik; I-TRAF
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206499 representing BC026148 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAAAACATTGGTGAGCAACTCAATAGAGCATATGAAGCCTTCCGACAGGCATGCATGGATAGAG ATTCAGCAGTAAGAGAGCTACAGCAAAAGACTGAGAACTATGAACAAAGAATACGCGAGCAACAGGAACA GCTGTCATTTCAACAAAACCTAATTGACAGGCTGAAATCACAGCTACTTCTCGTGGATTCTAGTCGAGAT AACAGTTATGGCTATGTACCTTTGCTTGAAGACAGTGACAGAAGGAAGAATAATTTGACCCTTGATGAAC CACATGATAAAGTGAAACTAGGAACACTGAGATATAAGCAATCAAAGGTGAGACGACAAGAAGTTTCTTC TGGAAAAGAATCCGCCAAGGGTCTCAACATCCCTCTGCATCACGAAAGGGATAATATAGAGAAGACTTTC TGGGACCTTAAAGAAGAATTTCATAGGATTTGCTTGCTAGCAAAAGCACAGAAAGACCACTTAAGCAAAC TTAATATACCAGATATTGCAACTGACACACAGTGTTCTGTGCCTATACAGTGTACTGATAAAACAGAGAA ACAAGAAGCGCTGTTTAAGCCCCAGGCTAAAGATGATATAAATAGAGGTATGTCGTGCGTCACAGCTGTC ACACCAAGAGGACTGGGCCGGGATGAGGAAGATACCTCTTTTGAATCACTTTCTAAATTCAATGTCAAGT TTCCGCCTATGGACAATGACTCTATTTTTCTACATAGCACTCCAGAGGCCCCAAGCATCCTTGCTCCTGC CACACCTGAGACAGTGTGCCAGGACCGATTTAATATGGAAGTCAGAGACAACCCAGGAAACTTTGTTAAA ACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAACTTCAGCTATACAAAACCTTAAAACAA CTGACAAAACAAACCCCTCAAATCTTAGAGCGCCGTGTTTGCCAGCTGGAGACCACAATGTGTTCTATGT AAATACGTTCCCACTTCAAGACCCGCCTGACGCACCTTTTCCCTCACTGGATTCCCCAGGAAAGGCTGTC CGAGGACCACAGCAGCCCTTTTGGAAGCCTTTTCTTAACCAAGACACTGACTTAGTGGTACCAAGTGATT CAGACTCAGAGCTCCTTAAACCTCTAGTGTGTGAATTCTGTCAAGAGCTTTTCCCACCATCCATTACATC CAGAGGGGATTTCCTCCGGCATCTTAATACACACTTTAATGGGGAGACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206499 representing BC026148 Red=Cloning site Green=Tags(s) MDKNIGEQLNRAYEAFRQACMDRDSAVRELQQKTENYEQRIREQQEQLSFQQNLIDRLKSQLLLVDSSRD NSYGYVPLLEDSDRRKNNLTLDEPHDKVKLGTLRYKQSKVRRQEVSSGKESAKGLNIPLHHERDNIEKTF WDLKEEFHRICLLAKAQKDHLSKLNIPDIATDTQCSVPIQCTDKTEKQEALFKPQAKDDINRGMSCVTAV TPRGLGRDEEDTSFESLSKFNVKFPPMDNDSIFLHSTPEAPSILAPATPETVCQDRFNMEVRDNPGNFVK TEETLFEIQGIDPITSAIQNLKTTDKTNPSNLRAPCLPAGDHNVFYVNTFPLQDPPDAPFPSLDSPGKAV RGPQQPFWKPFLNQDTDLVVPSDSDSELLKPLVCEFCQELFPPSITSRGDFLRHLNTHFNGET TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026148
ORF Size	1239 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026148, AAH26148
RefSeq Size	2084 bp
RefSeq ORF	1241 bp
Locus ID	21353
Gene Summary	Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.