Tead1 (BC060138) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TEF-1, TEAD-1, mTEF-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206462 representing BC060138 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGGATGAGCGACTCGGCAGATAAGCCGATTGACAACGACGCGGAGGGCGTCTGGAGTCCTGATA TTGAGCAGAGTTTCCAGGAGGCCCTGGCTATCTATCCGCCGTGTGGGAGGAGAAAAATCATCTTATCAGA CGAAGGCAAAATGTATGGTAGAAATGAATTGATAGCCAGATACATCAAACTCAGGACGGGAAAGACAAGG ACCAGGAAGCAGGTGTCTAGTCACATTCAGGTTCTTGCCAGAAGGAAATCTCGTGATTTTCATTCCAAGC TGAAGGATCAGACTGCCAAGGACAAGGCCCTGCAGCACATGGCTGCCATGTCATCAGCCCAGATCGTCTC GGCTACTGCCATCCACAACAAGCTGGGGCTGCCTGGGATTCCACGCCCCACCTTCCCGGGGGGTCCGGGG TTCTGGCCTGGGATGATACAGACAGGACAGCCAGGATCCTCACAAGACGTCAAGCCCTTTGTGCAGCAGG CCTACCCCATCCAGCCAGCAGTCACAGCCCCCATTCCAGGGTTTGAGCCTACGTCAGCCCCAGCCCCCTC AGTTCCTGCCTGGCAGGGCCGATCCATTGGCACAACCAAGCTTCGCCTGGTGGAATTCTCCGCTTTCCTT GAACAGCAGAGAGACCCAGACTCGTACAACAAACACCTCTTCGTGCACATCGGGCATGCCAACCATTCTT ACAGTGACCCGTTGCTCGAATCTGTGGACATTCGTCAGATATATGACAAATTTCCTGAAAAGAAAGGTGG CTTGAAGGAGCTGTTTGGAAAGGGCCCTCAAAACGCCTTCTTCCTCGTCAAATTCTGGGCGGACTTAAAC TGCAATATCCAAGACGACGCCGGGGCCTTTTATGGTGTGAGCAGTCAGTATGAGAGTTCTGAGAACATGA CAGTTACCTGTTCCACCAAAGTGTGCTCCTTTGGGAAACAAGTAGTAGAAAAAGTAGAGACGGAGTATGC GAGGTTCGAGAATGGTCGATTCGTGTACCGAATAAACCGCTCGCCAATGTGTGAATATATGATCAACTTC ATCCACAAGCTCAAACACCTACCAGAGAAATATATGATGAACAGTGTTTTGGAAAACTTCACCATATTAT TGGTGGTAACAAACAGGGATACACAAGAAACTCTGCTCTGCATGGCCTGTGTATTTGAAGTCTCGAATAG CGAACACGGAGCACAGCACCATATCTACAGGCTTGTGAAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206462 representing BC060138 Red=Cloning site Green=Tags(s) MERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTR TRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGGPG FWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPTSAPAPSVPAWQGRSIGTTKLRLVEFSAFL EQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLN CNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINF IHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060138
ORF Size	1235 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060138, AAH60138
RefSeq Size	2091 bp
RefSeq ORF	1235 bp
Locus ID	21676
Gene Summary	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif (By similarity).[UniProtKB/Swiss-Prot Function]

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