Tnfsf12 (BC096441) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Tnfsf12-Tnfsf13; Tnfsf12Tnfsf13; Tnfsf12tnfsf13; Twe-pril
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206410 representing BC096441 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCCGTCGGAGCCAGAGGCGGAGGGGGCGCCGGGGGGAGCCGGGCACCGCCCTGCTGGCCCCGC TGGTGCTGAGCCTGGGCCTGGCGCTGGCCTGCCTTGGCCTCCTGCTGGTCGTGGTCAGCCTGGGGAGCTG GGCAACGCTGTCTGCCCAGGAGCCTTCTCAGGAGGAGCTGACAGCAGAGGACCGCCGGGAGCCCCCTGAA CTGAATCCCCAGACAGAGGAAAGCCAGGATGTGGTACCTTTCTTGGAACAACTAGTCCGGCCTCGAAGAA GTGCTCCTAAAGGCCGGAAGGCGCGGCCTCGCCGAGCTATTGCAGCCCATTATGAGGTTCATCCTCGGCC AGGACAGGATGGAGCACAAGCAGGTGTGGATGGGACAGTGAGTGGCTGGGAAGAGACCAAAATCAACAGC TCCAGCCCTCTGCGCTACGACCGCCAGATTGGGGAATTTACAGTCATCAGGGCTGGGCTCTACTACCTGT ACTGTCAGGTGCACTTTGATGAGGGAAAGGCTGTCTACCTGAAGCTGGACTTGCTGGTGAACGGTGTGCT GGCCCTGCGCTGCCTGGAAGAATTCTCAGCCACAGCAGCAAGCTCTCCTGGGCCCCAGCTCCGTTTGTGC CAGACAGAGCTGCAAAGCCTAAGGCGGGAGGTGAGCCGGCTGCAGCGGAGTGGAGGGCCTTCCCAGAAGC AGGGAGAGCGCCCATGGCAGAGCCTCTGGGAGCAGAGTCCTGATGTCCTGGAAGCCTGGAAGGATGGGGC GAAATCTCGGAGAAGGAGAGCAGTACTCACCCAGAAGCACAAGAAGAAGCACTCAGTCCTGCATCTTGTT CCAGTTAACATTACCTCCAAGGACTCTGACGTGACAGAGGTGATGTGGCAACCAGTACTTAGGCGTGGGA GAGGCCTGGAGGCCCAGGGAGACATTGTACGAGTCTGGGACACTGGAATTTATCTGCTCTATAGTCAGGT CCTGTTTCATGATGTGACTTTCACAATGGGTCAGGTGGTATCTCGGGAAGGACAAGGGAGAAGAGAAACT CTATTCCGATGTATCAGAAGTATGCCTTCTGATCCTGACCGTGCCTACAATAGCTGCTACAGTGCAGGTG TCTTTCATTTACATCAAGGGGATATTATCACTGTCAAAATTCCACGGGCAAACGCAAAACTTAGCCTTTC TCCGCATGGAACATTCCTGGGGTTTGTGAAACTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206410 representing BC096441 Red=Cloning site Green=Tags(s) MAARRSQRRRGRRGEPGTALLAPLVLSLGLALACLGLLLVVVSLGSWATLSAQEPSQEELTAEDRREPPE LNPQTEESQDVVPFLEQLVRPRRSAPKGRKARPRRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEETKINS SSPLRYDRQIGEFTVIRAGLYYLYCQVHFDEGKAVYLKLDLLVNGVLALRCLEEFSATAASSPGPQLRLC QTELQSLRREVSRLQRSGGPSQKQGERPWQSLWEQSPDVLEAWKDGAKSRRRRAVLTQKHKKKHSVLHLV PVNITSKDSDVTEVMWQPVLRRGRGLEAQGDIVRVWDTGIYLLYSQVLFHDVTFTMGQVVSREGQGRRET LFRCIRSMPSDPDRAYNSCYSAGVFHLHQGDIITVKIPRANAKLSLSPHGTFLGFVKL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC096441
ORF Size	1226 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC096441, AAH96441
RefSeq Size	1511 bp
RefSeq ORF	1226 bp
Locus ID	619441
Gene Summary	This gene represents read-through transcription between neighboring genes encoding tumor necrosis factor family members. This results a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The homologous fusion protein in human is known to be membrane-anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines. Alternative splicing of this mouse gene results in multiple transcript variants. [provided by RefSeq, Oct 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.