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Pdk2 (NM_133667) Mouse Tagged ORF Clone
CAT＃: MG206400
- TrueORF®
Pdk2 (tGFP-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 2 (Pdk2)
ORF Plasmid: DDK
"NM_133667" in other vectors (4)
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CNY 3,140.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206400 representing NM_133667
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTGGGTCCGGGCGCTGTTGAAGAATGCGTCCCTGGCAGGGGCACCCAAGTACATCGAGCACTTCA GCAAGTTCTCCCCGTCCCCGTTGTCCATGAAGCAGTTTCTAGACTTCGGATCCAGCAATGCCTGCGAGAA GACGTCATTCACTTTCCTCCGGCAGGAGCTGCCCGTTCGCCTGGCCAACATCATGAAAGAGATCAACCTG CTTCCTGACCGAGTGCTGGGCACCCCATCGGTGCAGCTGGTGCAGAGCTGGTATGTCCAGAGTCTGCTGG ACATCATGGAATTCCTGGACAAAGACCCCGAGGACCACCGGACTCTAAGCCAGTTCACAGATGCCCTGGT CACCATCCGGAACCGGCACAATGACGTGGTGCCCACCATGGCACAGGGAGTGCTGGAGTACAAGGACACC TATGGAGATGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTCAGCCGCATCT CCATCCGAATGCTAATCAATCAACACACCCTCATCTTTGATGGCAGCACCAACCCAGCCCACCCCAAACA CATTGGCAGCATCGATCCCAACTGCAGCGTGTCTGATGTGGTGAAAGACGCCTATGACATGGCTAAGCTC CTGTGTGACAAGTATTACATGGCTTCCCCTGACCTGGAGATCCAGGAAGTCAATGCCACCAACGCCAACC AGCCCATTCACATGGTCTATGTCCCTTCCCACCTGTACCACATGCTCTTTGAACTCTTTAAGAATGCCAT GCGGGCCACTGTGGAAAGCCACGAGTCCAGCCTCACACTCCCCCCCATCAAGATTATGGTGGCCCTCGGT GAAGAAGATCTGTCCATCAAAATGAGTGACCGAGGCGGGGGTGTCCCCTTGAGGAAGATCGAGAGGCTCT TCAGCTACATGTACTCCACAGCTCCCACACCCCAGCCTGGCACTGGGGGTACCCCGCTGGCTGGCTTTGG GTACGGACTCCCCATTTCCCGCCTCTACGCCAAGTACTTCCAGGGGGACTTGCAGCTTTTCTCTATGGAG GGCTTTGGGACAGATGCTGTCATCTATCTGAAGGCCCTGTCCACGGACTCAGTGGAGCGCCTGCCTGTCT ACAACAAGTCTGCCTGGCGCCACTACCAGACCATCCAGGAGGCCGGCGACTGGTGTGTGCCCAGCACAGA GCCCAAGAACACATCGACGTATCGGGTCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206400 representing NM_133667
Red=Cloning site Green=Tags(s) MRWVRALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINL LPDRVLGTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDT YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCSVSDVVKDAYDMAKL LCDKYYMASPDLEIQEVNATNANQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLTLPPIKIMVALG EEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSME GFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133667 |
| ORF Size | 1221 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133667.2 |
| RefSeq Size | 2243 bp |
| RefSeq ORF | 1224 bp |
| Locus ID | 18604 |
| UniProt ID | Q9JK42 |
| Gene Summary | Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
,Li, J;Diao, B;Guo, S;Huang, X;Yang, C;Feng, Z;Yan, W;Ning, Q;Zheng, L;Chen, Y;Wu, Y;,
Nat Commun
,PubMed ID 29109438
[Pdk2]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203148 | Pdk2 (untagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 2 (Pdk2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR206400 | Pdk2 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 2 (Pdk2), nuclear gene encoding mitochondrial protein |
CNY 2,850.00 |
|
| MR206400L3 | Lenti ORF clone of Pdk2 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 2 (Pdk2), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR206400L4 | Lenti ORF clone of Pdk2 (mGFP-tagged) - Mouse pyruvate dehydrogenase kinase, isoenzyme 2 (Pdk2), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
