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Map2k1 (NM_008927) Mouse Tagged ORF Clone
CAT＃: MG206149
- TrueORF®
Map2k1 (tGFP-tagged) - Mouse mitogen activated protein kinase kinase 1 (Map2k1)
ORF Plasmid: DDK
"NM_008927" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MAPKK1; Mek1; MEKK1; Prkmk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206149 representing NM_008927
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGATGGCTCGGCGGTTAACGGGACCA GCTCGGCCGAGACCAACCTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTGGAGCTTGACGAGCAGCAGCG GAAGCGGCTCGAGGCCTTTCTGACGCAGAAGCAGAAGGTGGGGGAACTGAAGGATGATGACTTTGAGAAG ATCAGCGAACTGGGAGCTGGCAACGGTGGAGTGGTCTTCAAGGTCTCCCACAAGCCATCTGGCCTGGTTA TGGCTAGAAAGCTGATCCACCTGGAGATCAAACCCGCAATCCGGAACCAGATCATCCGGGAGCTGCAGGT ACTGCACGAGTGCAACTCCCCGTACATCGTGGGCTTCTACGGGGCCTTCTACAGCGACGGCGAGATCAGC ATCTGCATGGAGCACATGGATGGTGGGTCCTTGGATCAAGTTCTGAAGAAAGCTGGAAGAATTCCTGAGC AAATTTTAGGAAAAGTTAGCATTGCTGTGATAAAAGGCCTGACCTATCTTCGGGAGAAGCACAAGATTAT GCACAGAGATGTCAAGCCATCCAACATTCTAGTGAACTCACGTGGGGAGATCAAACTCTGTGATTTTGGG GTCAGCGGGCAGCTAATTGACTCTATGGCCAACTCCTTCGTGGGCACGAGATCCTACATGTCGCCTGAGA GACTCCAGGGGACTCACTACTCTGTGCAGTCGGACATCTGGAGCATGGGGCTCTCTCTGGTGGAGATGGC AGTTGGGAGATACCCCATTCCTCCTCCTGATGCCAAGGAGCTGGAGCTACTGTTTGGATGCCATGTGGAA GGAGACGCAGCCGAAACACCACCCAGGCCAAGGACCCCTGGGAGGCCTCTCAGCTCATATGGAATGGACA GCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATTGTCAATGAGCCTCCTCCAAAACTGCCCAG TGGAGTATTCAGTCTGGAGTTTCAGGATTTTGTGAATAAATGCTTAATAAAGAACCCTGCAGAGAGAGCA GATCTGAAGCAGCTCATGGTACATGCTTTCATCAAAAGATCTGACGCCGAGGAGGTAGACTTCGCAGGCT GGCTCTGCTCCACCATTGGGCTTAACCAGCCCAGCACACCAACCCACGCTGCCAGCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206149 representing NM_008927
Red=Cloning site Green=Tags(s) MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEK ISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEIS ICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFG VSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCHVE GDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERA DLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAASI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008927 |
| ORF Size | 1179 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008927.4 |
| RefSeq Size | 2388 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 26395 |
| UniProt ID | P31938 |
| Gene Summary | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202574 | Map2k1 (untagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1), (10ug) |
CNY 2,000.00 |
|
| MR206149 | Map2k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) |
CNY 3,330.00 |
|
| MR206149L3 | Lenti ORF clone of Map2k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) |
CNY 5,230.00 |
|
| MR206149L4 | Lenti ORF clone of Map2k1 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) |
CNY 5,230.00 |
