United States
Germany
Japan
United Kingdom
China
Map2k1 (NM_008927) Mouse Untagged Clone
CAT＃: MC202574
Map2k1 (untagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | MAPKK1; Mek1; MEKK1; Prkmk1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC054754 sequence for NM_008927
CGAGCCGCCCCGACTCTGGGCAGAGCCGAGGGAGGAAGCGAGAAGCGGCCGCGCGCTCCCTGCTGAGTTG CAGGCTCTTTCCCGGCTGCAAGATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGAT GGCTCGGCGGTTAACGGGACCAGCTCGGCCGAGACCAACCTGGAGGCCTTGCAGAAGAAGCTGGAGGAGC TGGAGCTTGACGAGCAGCAGCGGAAGCGGCTCGAGGCCTTTCTGACGCAGAAGCAGAAGGTGGGGGAACT GAAGGATGATGACTTTGAGAAGATCAGCGAACTGGGAGCTGGCAACGGTGGAGTGGTCTTCAAGGTCTCC CACAAGCCATCTGGCCTGGTTATGGCTAGAAAGCTGATCCACCTGGAGATCAAACCCGCAATCCGGAACC AGATCATCCGGGAGCTGCAGGTACTGCACGAGTGCAACTCCCCGTACATCGTGGGCTTCTACGGGGCCTT CTACAGCGACGGCGAGATCAGCATCTGCATGGAGCACATGGATGGTGGGTCCTTGGATCAAGTTCTGAAG AAAGCTGGAAGAATTCCTGAGCAAATTTTAGGAAAAGTTAGCATTGCTGTGATAAAAGGCCTGACCTATC TTCGGGAGAAGCACAAGATTATGCACAGAGATGTCAAGCCATCCAACATTCTAGTGAACTCACGTGGGGA GATCAAACTCTGTGATTTTGGGGTCAGCGGGCAGCTAATTGACTCTATGGCCAACTCCTTCGTGGGCACG AGATCCTACATGTCGCCTGAGAGACTCCAGGGGACTCACTACTCTGTGCAGTCGGACATCTGGAGCATGG GGCTCTCTCTGGTGGAGATGGCAGTTGGGAGATACCCCATTCCTCCTCCTGATGCCAAGGAGCTGGAGCT ACTGTTTGGATGCCATGTGGAAGGAGACGCAGCCGAAACACCACCCAGGCCAAGGACCCCTGGGAGGCCT CTCAGCTCATATGGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATTGTCAATG AGCCTCCTCCAAAACTGCCCAGTGGAGTATTCAGTCTGGAGTTTCAGGATTTTGTGAATAAATGCTTAAT AAAGAACCCTGCAGAGAGAGCAGATCTGAAGCAGCTCATGGTACATGCTTTCATCAAAAGATCTGACGCC GAGGAGGTAGACTTCGCAGGCTGGCTCTGCTCCACCATTGGGCTTAACCAGCCCAGCACACCAACCCACG CTGCCAGCATCTGAGCCTTTAGGAAGCAGCAAAGAGGAATTCTCTGCCCAGTGGCATGCCATGTTGCTTT CAGGCCTCTCCCATGCTTGTCTATGTTCAGACGTGCATCTCATCTGTGACAAAGGATGAAGAACACAGCA TGTGCCAAATTGTACTTGTGTCATTTTTAATATCATTGTCTTTATCACTATGGTTACTCCCCTAAGTGGA TTGGCTTTGTGCTTGGGGCTATTTGTCTGTTCATCAAACACATGCCAGGCTGAACTACAGTGAAACCCTA GTGACCTGGGTGGTCGTTCTTACTGATGTTTGCACTGCTGTTCATCGTGACTCACTAGCTGGCTGCCTGT ATTGTCAGGATTCTCGGACCTTGGTACTTCACTCTTGCTGGTGACCTCTCAGTCTGAGAGGGAGCCTTGT GAGACCCTTCACAGGCAGTGCATGCATGGAAAGCATGCTTTGCTGCTACTGAAATGAGCATCAGAACGTG TACGTCATGGTATTTTTATTTTTTGCTTTTGGTATAGAACTCAGCAATTCCCATCAAAAAAACCTAAGCA GAGCCCATCACTGCCATGATAGCTGGGCTTCAGTCTGTCTACTGTGGTGATTTTTAGACTTCTGGTTGTA TTTCTATATTTATTTTTAAATATACAGTGTGGGATACTTAGTGGTGTGTGTCTCTAAGTTTGGATTAGTG TTTCTAAATTGGTGGTTATTTTGAATGTCACAAATGGATTAAAGCATCAATGTATCAAGAGTTCTATCTT TCTTCCAGTCTAAGTACCAATGCTATTGTAAACAACGTGTATAGTGCCTACAAATTGTATGAAACCCCTT TTAACCACTTTAATCAAGATGTTTATCAAATCTAATCTCTTATTCTAATAAAAATACTATCAAGTTAAAA TAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_008927 |
| Insert Size | 1182 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054754, AAH54754 |
| RefSeq Size | 2189 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 26395 |
| UniProt ID | P31938 |
| Gene Summary | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206149 | Map2k1 (tGFP-tagged) - Mouse mitogen activated protein kinase kinase 1 (Map2k1) |
CNY 3,710.00 |
|
| MR206149 | Map2k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) |
CNY 3,656.00 |
|
| MR206149L3 | Lenti ORF clone of Map2k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) |
CNY 6,056.00 |
|
| MR206149L4 | Lenti ORF clone of Map2k1 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) |
CNY 6,056.00 |
