Csnk2a1 (BC060742) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Csnk2a1-rs4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206103 representing BC060742 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAACACACACAGACCCCGAGAGTACT GGGATTATGAATCACATGTGGTGGAATGGGGGAATCAAGATGACTATCAGCTTGTTCGAAAATTAGGTAG GGGCAAATACAGTGAAGTGTTTGAAGCCATCAACATCACAAATAATGAAAAAGTTGTTGTTAAAATTCTC AAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAGAGGTGGGCCCAACA TCATCACACTTGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCTGCCTTGGTTTTTGAACATGTAAA CAACACAGACTTCAAGCAATTGTACCAGACGTTAACAGACTATGACATTCGATTTTACATGTATGAAATT CTGAAAGCCCTGGATTATTGTCACAGCATGGGGATTATGCACAGAGATGTGAAACCCCATAATGTCATGA TTGATCATGAGCACAGAAAGCTTCGGCTAATAGACTGGGGTTTAGCTGAGTTTTACCATCCAGGCCAAGA GTATAATGTCCGAGTTGCTTCTCGATATTTCAAAGGTCCAGAGCTACTTGTAGATTATCAGATGTACGAT TATAGTTTGGATATGTGGAGCTTGGGTTGTATGCTGGCCAGTATGATCTTCCGGAAGGAGCCATTTTTCC ATGGACATGACAATTATGATCAGTTGGTGAGGATAGCCAAGGTTCTGGGAACAGAAGATTTATATGACTA TATTGACAAGTACAACATTGAATTAGATCCACGTTTCAACGATATCTTGGGCAGACACTCCCGTAAGCGA TGGGAACGCTTTGTCCACAGTGAAAACCAGCATCTTGTCAGCCCTGAGGCCTTGGATTTTCTGGACAAGC TGCTTCGATATGACCACCAGTCACGGCTCACTGCAAGAGAGGCCATGGAGCACCCTTACTTCTACACTGT TGTGAAGGACCAGGCTCGAATGAGTTCCACTAGCATGGCAGGGGGCAGCACACCTGTCAGCAGCGCCAAT ATGATGTCAGGGATTTCTTCAGTGCCAACTCCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTG CCGCTGCCAACTCACTTGGGATACCTGTTCCAGCTGCTGCTGGCGCTCAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206103 representing BC060742 Red=Cloning site Green=Tags(s) MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKIL KPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEI LKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYD YSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKR WERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMSSTSMAGGSTPVSSAN MMSGISSVPTPSPLGPLAGSPVIAAANSLGIPVPAAAGAQQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060742
ORF Size	1175 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060742, AAH60742
RefSeq Size	4213 bp
RefSeq ORF	1175 bp
Locus ID	12995
Gene Summary	Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Feb 2014]

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