Dpf1 (NM_013874) Mouse Tagged ORF Clone
CAT#: MG206060
- TrueORF®
Dpf1 (tGFP-tagged) - Mouse neuronal d4 domain family member (Neud4)
ORF Plasmid: DDK
"NM_013874" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Neud4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206060 representing NM_013874
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCGCCATTCAGAACCCGCTCAAGTCCCTTGGCGAGGACTTCTACCGGGAGGCCATCGAGCACT GTCGCAGCTACAACGCGCGCCTGTGTGCCGAGCGCAGCCTGCGCCTGCCTTTCCTCGACTCGCAGACCGG AGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGACCCACCGCGGGCCTGGTTTGGCCCCGGGACAG ATCTACACTTACCCCGCCCGCTGTTGGAGGAAGAAACGGAGACTCAACATCCTGGAGGACCCCAGGCTCC GGCCCTGCGAGTACAAGATCGATTGTGAGGCACCTCTGAAGAAGGAGGGTGGCCTCCCGGAAGGGCCAGT CCTCGAGGCTCTGCTGTGTGCTGAGACTGGAGAGAAGAAAGTGGAGCTGAAGGAGGAGGAGACCATCATG GACTGTCAGAAACAGCAGTTGCTGGAGTTTCCGCATGATCTCGAGGTAGAAGACTTGGAGGAAGACATTC CCAGGAGGAAGAACAGGGCAAGAGGAAAGGCATATGGCATTGGAGGTCTCCGCAAACGCCAGGACACCGC ATCCCTGGAGGACCGAGACAAGCCGTACGTCTGTGATATCTGTGGGAAGAGATATAAGAACCGGCCAGGA CTCAGCTACCATTACACCCACACCCACCTGGCTGAGGAGGAGGGGGAGGAGCACACTGAACGCCACGCCC TGCCTTTCCACCGGAAAAACAACCATAAACAGTTTTACAAAGAATTGGCCTGGGTCCCCGAGGCACAGAG GAAACACACAGCCAAGAAAGCACCAGATGGCACTGTCATCCCCAATGGCTACTGTGACTTTTGCCTGGGG GGCTCCAAGAAGACTGGGTGTCCCGAGGACCTCATCTCCTGTGCGGACTGTGGGCGATCAGGACATCCCT CGTGTTTACAGTTCACGGTGAACATGACCGCGGCTGTGCGGACCTACCGCTGGCAGTGCATTGAATGCAA GTCCTGCAGCCTGTGTGGCACCTCGGAGAATGACGACCAGCTGCTGTTCTGTGATGACTGCGATCGAGGT TACCACATGTACTGCCTGAGCCCTCCCATGGCGGAGCCCCCGGAAGGGAGCTGGAGCTGCCACCTCTGTC TCCGGCACTTGAAGGAAAAGGCCTCTGCTTACATCACCCTGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206060 representing NM_013874
Red=Cloning site Green=Tags(s) MATAIQNPLKSLGEDFYREAIEHCRSYNARLCAERSLRLPFLDSQTGVAQNNCYIWMEKTHRGPGLAPGQ IYTYPARCWRKKRRLNILEDPRLRPCEYKIDCEAPLKKEGGLPEGPVLEALLCAETGEKKVELKEEETIM DCQKQQLLEFPHDLEVEDLEEDIPRRKNRARGKAYGIGGLRKRQDTASLEDRDKPYVCDICGKRYKNRPG LSYHYTHTHLAEEEGEEHTERHALPFHRKNNHKQFYKELAWVPEAQRKHTAKKAPDGTVIPNGYCDFCLG GSKKTGCPEDLISCADCGRSGHPSCLQFTVNMTAAVRTYRWQCIECKSCSLCGTSENDDQLLFCDDCDRG YHMYCLSPPMAEPPEGSWSCHLCLRHLKEKASAYITLT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013874 |
ORF Size | 1164 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013874.2, NP_038902.1 |
RefSeq Size | 2278 bp |
RefSeq ORF | 1167 bp |
Locus ID | 29861 |
UniProt ID | Q9QX66 |
Gene Summary | May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205044 | Dpf1 (untagged) - Mouse D4, zinc and double PHD fingers family 1 (Dpf1), (10ug) |
CNY 3,656.00 |
|
MR206060 | Dpf1 (Myc-DDK-tagged) - Mouse D4, zinc and double PHD fingers family 1 (Dpf1) |
CNY 3,656.00 |
|
MR206060L3 | Lenti ORF clone of Dpf1 (Myc-DDK-tagged) - Mouse D4, zinc and double PHD fingers family 1 (Dpf1) |
CNY 5,230.00 |
|
MR206060L4 | Lenti ORF clone of Dpf1 (mGFP-tagged) - Mouse D4, zinc and double PHD fingers family 1 (Dpf1) |
CNY 5,230.00 |