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Maea (BC039054) Mouse Tagged ORF Clone
CAT＃: MG206018
- TrueORF®
Maea (tGFP-tagged) - Mouse macrophage erythroblast attacher (cDNA clone MGC:6504 IMAGE:2648667)
ORF Plasmid: DDK
"BC039054" in other vectors (4)
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CNY 4,180.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110030D19Rik; EMP; Gid9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206018 representing BC039054
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGAAGGTGCAGGAGTACCCGACCCTCAAGGTGCCCTATGAGACACTGAACAAACGCTTCCGAG CTGCTCAGAAAAACATCGATCGAGAGACTAGCCACGTCACCATGGTGGTAGCTGAGCTTGAGAAGACCTT GAGTAGTTGCCCAGCTGTGGACTCTGTGGTCAGCCTATTGGATGGTGTGGTGGAGAAGCTGAGTGTCCTC AAGAGGAAGGCAGTAGAGTCCATCCAGGCCGAGGATGAGAGCGCCAAGCTCTGCAAACGTAGGATCGAGC ACCTCAAGGAGCACAGCAGTGACCAGCCAGCAGCAGCCAGCATGTGGAAGCGGAAGCGCATGGACCGGAT GATGGTGGAGCATCTGCTACGCTGTGGCTACTACAACACAGCTGTGAAGCTGGCTCGCCAGAGTGGCATC GAGGACCTTGTGAATATCGAGATGTTCCTGACAGCCAAAGAAGTGGAGGAGTCCTTGGAGAGGCGTGAGA CAGCCACCTGCCTTGCCTGGTGCCATGATAACAAGTCCCGACTCCGGAAGATGAAGAGCTGCCTAGAGTT CAGCCTCAGGATTCAGGAGTTCATTGAACTTGTCCGGCAGAACAAGCGCCTGGATGCTGTGAGACATGCA AGAAAGCACTTCAGTCAGGCTGAAGGGAGCCAGCTGGATGAGGTCCGCCAGGTCATGGGCATGTTGGCCT TCCCACCAGACACACATATCTCCCCATACAAGGACCTCCTGGACCCAGCCCGGTGGCGAATGCTGATCCA GCAGTTTCGATATGATAACTACCGGCTGCACCAGCTGGGAAACAGCTCAGTCTTCACCCTCACCCTGCAG GCTGGGCTCTCAGCAATAAAGACACCACAGTGCTACAAGGAGGATGGCAGCTCTAAGAGCCCTGACTGCC CTGTGTGCAGCCGCTCTCTGAACAAACTGGCACAGCCCCTGCCCATGGCTCACTGTGCCAACTCCCGCCT GGTCTGCAAGATCTCTGGTGACGTGATGAATGAGAATAACCCACCCATGATGCTGCCTAATGGCTATGTC TATGGCTACAATTCTCTGCTTTCTATTCGTCAAGATGATAAAGTTGTTTGCCCAAGAACCAAAGAAGTCT TCCACTTCTCCCAAGCTGAGAAAGTATACATCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206018 representing BC039054
Red=Cloning site Green=Tags(s) MTLKVQEYPTLKVPYETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSSCPAVDSVVSLLDGVVEKLSVL KRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAASMWKRKRMDRMMVEHLLRCGYYNTAVKLARQSGI EDLVNIEMFLTAKEVEESLERRETATCLAWCHDNKSRLRKMKSCLEFSLRIQEFIELVRQNKRLDAVRHA RKHFSQAEGSQLDEVRQVMGMLAFPPDTHISPYKDLLDPARWRMLIQQFRYDNYRLHQLGNSSVFTLTLQ AGLSAIKTPQCYKEDGSSKSPDCPVCSRSLNKLAQPLPMAHCANSRLVCKISGDVMNENNPPMMLPNGYV YGYNSLLSIRQDDKVVCPRTKEVFHFSQAEKVYIM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC039054 |
| ORF Size | 1158 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC039054.1 |
| RefSeq Size | 2128 bp |
| RefSeq ORF | 1157 bp |
| Locus ID | 59003 |
| Gene Summary | Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (By similarity). Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (PubMed:16707498). Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (By similarity). Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis (PubMed:16707498, PubMed:17071116). Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (PubMed:16707498). May contribute to nuclear architecture and cells division events (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218348 | Maea (untagged) - Mouse macrophage erythroblast attacher (cDNA clone MGC:6504 IMAGE:2648667), (10ug) |
CNY 6,940.00 |
|
| MR206018 | Maea (Myc-DDK-tagged) - Mouse macrophage erythroblast attacher (cDNA clone MGC:6504 IMAGE:2648667) |
CNY 3,800.00 |
|
| MR206018L3 | Lenti ORF clone of Maea (Myc-DDK-tagged) - Mouse macrophage erythroblast attacher (cDNA clone MGC:6504 IMAGE:2648667) |
CNY 5,700.00 |
|
| MR206018L4 | Lenti ORF clone of Maea (mGFP-tagged) - Mouse macrophage erythroblast attacher (cDNA clone MGC:6504 IMAGE:2648667) |
CNY 5,700.00 |
