Psat1 (NM_177420) Mouse Tagged ORF Clone
CAT#: MG205716
- TrueORF®
Psat1 (tGFP-tagged) - Mouse phosphoserine aminotransferase 1 (Psat1)
ORF Plasmid: DDK
"NM_177420" in other vectors (4)
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CNY 3,710.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D8Ertd814e; EPIP; PSA; Psat |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205716 representing NM_177420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCCACCAAGCAAGTGGTTAACTTTGGGCCCGGGCCTGCCAAGCTGCCACACTCGGTATTGTTGG AGATCCAGAAGCAGCTACTAGACTACAGAGGACTCGGCATCAGTGTGCTCGAAATGAGTCACAGGTCATC AGATTTTGCCAAGATTATTGGCAATACAGAGAATCTTGTGAGGGAATTGCTAGCTGTTCCCAACAACTAC AAGGTGATCTTTGTACAAGGAGGTGGGTCTGGCCAGTTCAGTGCTGTCCCCTTAAATCTGATTGGCCTGA AAGCTGGAAGGAGTGCTGACTACGTGGTGACCGGAGCTTGGTCAGCTAAGGCTGCGGAAGAAGCCAAGAA GTTTGGAACGGTGAACATTGTCCACCCTAAACTTGGAAGTTACACAAAAATTCCAGACCCAAGCACCTGG AACCTCAACCCGGACGCCTCCTATGTATACTTCTGTGCAAACGAGACTGTGCACGGGGTGGAGTTTGACT TCGTACCTGACGTCAAGGGAGCGGTGCTGGTCTGTGACATGTCCTCAAACTTCTTATCCAGGCCGGTGGA TGTTTCCAAGTTTGGTGTGATTTTCGCTGGTGCTCAGAAGAATGTTGGCTCTGCCGGGGTGACGGTGGTG ATTGTCCGGGATGACCTGCTGGGGTTCTCGCTCAGAGAGTGCCCATCAGTCCTTGACTACAAAGTGCAGG CTGGGAACAACTCTTTGTATAACACACCTCCGTGCTTCAGCATCTACGTCATGGGCATGGTCCTGGAATG GATCAAGAACAACGGCGGGGCCGCAGCCATGGAGAAGCTCAGCTCCATCAAATCCCAAATGATTTATGAG ATCATTGATAATTCTCAAGGATTTTATGTATGCCCAGTGGAGCGCCAGAATAGAAGCAGGATGAACATCC CATTTCGCATTGGCAACGCCAAAGGAGACGAAGCTTTGGAAAAGCGGTTTCTTGACAAGGCGGTAGAACT CAACATGATCTCCTTGAAGGGGCACAGGTCAGTGGGAGGCATTCGTGCCTCTCTGTATAACGCTGTCACA ACCGAAGACGTTGAGAAGCTGGCGGCCTTCATGAAGAATTTCTTGGAGATGCATCAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205716 representing NM_177420
Red=Cloning site Green=Tags(s) MEATKQVVNFGPGPAKLPHSVLLEIQKQLLDYRGLGISVLEMSHRSSDFAKIIGNTENLVRELLAVPNNY KVIFVQGGGSGQFSAVPLNLIGLKAGRSADYVVTGAWSAKAAEEAKKFGTVNIVHPKLGSYTKIPDPSTW NLNPDASYVYFCANETVHGVEFDFVPDVKGAVLVCDMSSNFLSRPVDVSKFGVIFAGAQKNVGSAGVTVV IVRDDLLGFSLRECPSVLDYKVQAGNNSLYNTPPCFSIYVMGMVLEWIKNNGGAAAMEKLSSIKSQMIYE IIDNSQGFYVCPVERQNRSRMNIPFRIGNAKGDEALEKRFLDKAVELNMISLKGHRSVGGIRASLYNAVT TEDVEKLAAFMKNFLEMHQL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177420 |
ORF Size | 1110 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177420.2, NP_803155.1 |
RefSeq Size | 2172 bp |
RefSeq ORF | 1113 bp |
Locus ID | 107272 |
UniProt ID | Q99K85 |
Gene Summary | Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Ferroptosis is governed by differential regulation of transcription in liver cancer
,null,
Redox Biology
,PubMed ID 31108460
[Psat1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200441 | Psat1 (untagged) - Mouse phosphoserine aminotransferase 1 (Psat1), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
MR205716 | Psat1 (Myc-DDK-tagged) - Mouse phosphoserine aminotransferase 1 (Psat1), transcript variant 1 |
CNY 3,656.00 |
|
MR205716L3 | Lenti ORF clone of Psat1 (Myc-DDK-tagged) - Mouse phosphoserine aminotransferase 1 (Psat1), transcript variant 1 |
CNY 5,230.00 |
|
MR205716L4 | Lenti ORF clone of Psat1 (mGFP-tagged) - Mouse phosphoserine aminotransferase 1 (Psat1), transcript variant 1 |
CNY 5,230.00 |