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Adprhl2 (NM_133883) Mouse Tagged ORF Clone
CAT＃: MG205710
- TrueORF®
Adprhl2 (tGFP-tagged) - Mouse ADP-ribosylhydrolase like 2 (Adprhl2)
ORF Plasmid: DDK
"NM_133883" in other vectors (4)
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CNY 5,256.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI836109; Arh3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205710 representing NM_133883
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGGCTGCGGCGGCAGCAGCTACAGCGATGTCGGCGGCGGGGGGCGGCGGGGCAAGTGCGGCCC GCTCCATCTCGCGCTTCCGAGGTTGCCTGGCGGGCGCGCTGCTGGGAGATTGCGTGGGCGCTGTCTACGA GGCACACGATACCGTCAGCCTGGCATCAGTCCTGAGTCACGTCGAGAGCCTGGAGCCGGACCCGGGCACG CCGGGCAGCGCGCGGACACAGACACTGTACTACACAGATGACACTGCCATGACCAGGGCCCTGGTGCAGT CCCTGCTGGCCAAGGAGGCCTTCGACGAGGTGGACATGGCTCACAGGTTTGCCCAGGAATACAAGAAGGA CCCTGACAGAGGGTATGGGGCCGGAGTCATCACTGTCTTCAAGAAACTCCTGAATCCCAAGTGCCGTGAT GTCTATGAGCCTGCCCGGGCCCAGTTCAACGGGAAGGGTTCCTATGGCAATGGGGGTGCCATGCGGGTAG CAGGCATCTCGCTGGCCTATAGCAGTGTCCAAGATGTACAGAAGTTTGCCCGGCTCTCGGCCCAGCTGAC CCACGCCTCTTCCCTGGGCTATAACGGTGCCATCTTGCAGGCCCTGGCTGTGCACCTTGCTCTGCAGGGT GTATCATCCAGTGAGCACTTCCTTGAGCAGCTTCTGGGCCACATGGAGGAGCTGGAAGGTGATGCCCAAT CGGTCTTGGACGCCAAGGAGTTGGGTATGGAGGAGCGTCCGTACTCCAGCAGGCTGAAGAAGGTCGGAGA GCTGCTGGACCAGGACGTGGTGAGCCGAGAGGAAGTGGTGTCCGAGCTAGGGAATGGCATTGCCGCCTTT GAATCTGTGCCCACCGCCATCTACTGCTTCCTGCGCTGCATGGAGCCTCACCCTGAGATCCCCTCCACCT TCAACAGTCTCCAGAGGACTCTCATCTACTCCATCTCACTTGGTGGGGACACAGACACCATAGCCACCAT GGCTGGGGCCATTGCTGGAGCTTACTATGGGATGGAACAGGTGCCGGAGAGCTGGCAGCAAAGTTGTGAA GGCTTTGAGGAGACAGACGTCCTGGCCCAGAGCCTGCACCGAGTCTTCCAGGAGAGCTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205710 representing NM_133883
Red=Cloning site Green=Tags(s) MAVAAAAAATAMSAAGGGGASAARSISRFRGCLAGALLGDCVGAVYEAHDTVSLASVLSHVESLEPDPGT PGSARTQTLYYTDDTAMTRALVQSLLAKEAFDEVDMAHRFAQEYKKDPDRGYGAGVITVFKKLLNPKCRD VYEPARAQFNGKGSYGNGGAMRVAGISLAYSSVQDVQKFARLSAQLTHASSLGYNGAILQALAVHLALQG VSSSEHFLEQLLGHMEELEGDAQSVLDAKELGMEERPYSSRLKKVGELLDQDVVSREEVVSELGNGIAAF ESVPTAIYCFLRCMEPHPEIPSTFNSLQRTLIYSISLGGDTDTIATMAGAIAGAYYGMEQVPESWQQSCE GFEETDVLAQSLHRVFQESS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133883 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133883.2, NP_598644.1 |
| RefSeq Size | 1368 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 100206 |
| UniProt ID | Q8CG72 |
| Gene Summary | ADP-ribose glycohydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose (By similarity). Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response (By similarity). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (By similarity). Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds (By similarity). Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos (PubMed:24191052). Also hydrolyzes free poly(ADP-ribose) in mitochondria (By similarity). Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins (By similarity). Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212053 | Adprhl2 (untagged) - Mouse ADP-ribosylhydrolase like 2 (Adprhl2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
| MR205710 | Adprhl2 (Myc-DDK-tagged) - Mouse ADP-ribosylhydrolase like 2 (Adprhl2), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| MR205710L3 | Lenti ORF clone of Adprhl2 (Myc-DDK-tagged) - Mouse ADP-ribosylhydrolase like 2 (Adprhl2), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR205710L4 | Lenti ORF clone of Adprhl2 (mGFP-tagged) - Mouse ADP-ribosylhydrolase like 2 (Adprhl2), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
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